The author reported clinical characteristic features and risk factors for acute intestinal infections in 225 children (140 boys and 85 girls, mean age 1.5 years old). 90 (40%) patients aged up to 12 months, 101 (44.9%) were from 1 to 2 years old, and 34 (15.1%) were more than 2 years old. Most children were hospitalized in the first week of illness and had moderate severe course of disease. Severe forms of illness reliably more often were noted in children being on artificial feeding. Toxicdystrophic condition, severe forms and perinatal encephalopathy were prevailed in boys. Factorial analysis revealed 20 most significant factors with factorization fullness 70.8%. The most significant factors were F1( factor of hyperthermia duration) with 8.5% effect, F2 (factor of pain syndrome) 5.8%, F3 (factor of normal enzymatic activity of colon bacillus) 4.9%, F4 (etiological factor) 4.6%, as well as F5( factor of opportunistic flora of the intestine) 4.4%, respectively., Mukarram Shadjalilova, and Literatura
Práce se zabývá interferencí dobutaminu s metodami využívajícími princip Trinderovy reakce a podmínkami získání vzorku u pacientů v intenzivní péči. Dobutamin výrazně snižuje koncentrace některých analytů (kreatinin stanovený enzymově, kyselina močová, triacylglyceroly, cholesterol, lipáza). Interference se projeví pouze při hrubém porušení zásad pro odběr vzorků., The main goal of the study is to examine analytical interference caused by dobutamine influencing methods based on Trinder peroxidase reaction and possible preanalytical pitfalls in Intensive Care Units. Dobutamine interference has significant impact on some laboratory tests, causing falsely low values upon determination of: Creatinine - enzymatic method, Uric acid, Triglycerides, Cholesterol and Lipase. The phenomenon occurs predominantly as a result of violating specimen collection rules., Sečník P. Jr., Franeková J., Komínková M., Kotrbatý J., Hunal Z., Tlučhořová D., Jabor A., and Literatura 5
Interference způsobené hemolýzou, chylozitou a ikterickým zbarvením vzorku představují dlouhodobý problém, který ovlivňuje kvalitu výsledků vydávaných v klinické biochemii. V laboratoři s preanalytickou automatizací bylo nezbytné nahradit vizuální popisy vzorků měřením sérových indexů (SI). V této práci jsou uvedeny komentáře popisující interference vzorku generované na základě naměřených SI a hodnoty sérových indexů pro jednotlivé metody, které po překročení vedou k nevydání výsledku tak, jak se používají na Oddělení biochemie a hematologie ve Fakultní nemocnici Brno. Vliv interferencí je také demonstrován na vzorcích pacientů., Interference caused by hemolysis, turbidity of lipemia and bilirubinemia of the sample represents a long-term problem that affects the quality of results given in clinical biochemistry. It was necessary to replace visual qualification of specimens with serum index (SI) measurement in the laboratory with preanalytical automation. This work contains comments describing sample interference generated based on measured SI and the values of serum indexes for individual methods that, when exceeded, lead to results that are no longer released, as used in the Department of Biochemistry and Hematology at the Brno University hospital (FN Brno). The influence of interference is also demonstrated on patient samples., Beňovská M., Dastych M., Čermáková Z., Tůmová J., and Lit.: 4
Cíl studie: Zavést metodu měření reverzního transportu cholesterolu (RTC) a stanovit jeho hodnoty v populaci. Metoda: Měření effl uxu radioaktivního cholesterolu z předem označených makrofágů 14C cholesterolem ve tkáňové kultuře. Porovnat tyto hodnoty s lipoproteinovými parametry. Výsledky: Průměrná hodnota RTC byla 12,51 ± 2,74 % radioaktivity přenesené na sérum testovaných osob. V celém souboru hodnoty RTC nekorelovaly s koncentrací celkového cholesterolu, ale významně pozitivně korelovaly s koncentrací HDL cholesterolu a zejména s koncentrací apoproteinu A1 (p < 0,01). Hodnota RTC navíc klesá s nadváhou a obezitou. Závěr: Přímé stanovení RTC zpřesňuje odhad individuálního rizika klinických komplikací aterosklerózy v porovnání s koncentrací HDL cholesterolu a má význam zejména pro výzkum., Objective: To introduce a direct method of reverse cholesterol transport (RCT) measurement and to determine RCT data in population. Material and methods: Measurement of radio-labeled cholesterol effl ux from pre-labeled macrophages in tissue culture. Comparison of RCT data to lipoprotein parameters is outlined. Results: The mean of RCT was 12.51 ± 2.74 % of radioactivity transferred from macrophages to serum of tested individuals. In the whole set of tested individuals RCT values were not related to total cholesterol concentration but signifi cant positive correlation was documented to HDL cholesterol and namely to apoprotein A1 concentration (p < 0.01). RCT decreases with overweight and obesity. Conclusion: Direct RCT measurement improves an estimation of risk of atherosclerosis clinical complications in comparison to HDL cholesterol concentration and it is an adequate tool for research., Poledne R., Králová Lesná I., and Lit.: 10
Kalibrátory a kontrolní materiály jsou členy společné rodiny referenčních materiálů a liší se pouze růzností zamýšlených účelů použití. Při jejich hodnocení proto musí být používáno stejných měřítek včetně hodnocení jejich komutability. Obvyklý přístup, hledající veškeré zdroje chyb a problémů v nekomutabilitě kontrolních materiálů, je alibistický, nepřijatelně zjednodušený a metrologicky nekompetentní. Výrobky IVD jsou nenahraditelným východiskem činnosti klinické laboratoře, avšak musí být přístupné kritickému hodnocení. Je to jeden z možných zdrojů jejich dalšího zlepšování., Both calibrators and control materials are members of reference materials family. They are only intended for different purposes. All characteristics of both kinds of materials including commutability should be assessed according to the same criteria. Usually seeking the problems of IVD dominantly in non-commutability of control materials is too simplified and shows insufficient knowledge of metrology in chemistry., Bedřich Friedecký, and Lit. 22
OBJECTIVES: The purpose of this study was to evaluate the in vitro sealing ability of three repair materials. Mineral trioxide aggregate (MTA; Group A), calcium phosphate cement (CPC; Group B), and light cured glass ionomer cement (GIC; Group C) when used to repair the perforation created in the pulpal floor of fifty extracted human permanent molars. MATERIALS AND METHODS: Preparation of access openings and furcation perforations were done, and the teeth divided into five experimental groups (A, B, C) including two controls (D, E) with ten samples in each group randomly. Following the repair procedure, the pulp chambers and access openings were filled with composite resin and immersed in 2% methylene blue solution for 48 hours. The teeth were sectioned longitudinally and the linear dye penetration measured under a stereomicroscope. RESULTS: The comparison of the linear length of micro-leakage (mm) among the experimental groups revealed no significant difference (p = 0.332). On calculating the percentage of depth of leakage to the total length of the perforation, it was observed that the mean leakage was 35.5% in Group A, 53.6% in Group B and the highest, 87.5% in Group C. The mean of leakage percentage was statistically significant by Kruskal-Wallis test (p = 0.003). The results indicated that the dye penetration used as furcation perforation repair material was least with mineral trioxide aggregate. Comparing the depth of penetration of dye, 50% of the Group A samples showed less than 25% of depth penetration. While 40% of Group B cases had more than 50% dye penetration. In our study, all Group C teeth had > or = 50% dye penetration. CONCLUSIONS: The present study indicated that GIC had the greatest dye penetration followed by CPC and MTA. Mineral trioxide aggregate and calcium phosphate cement had comparatively better sealing ability than glass ionomer cement. and P. Singh, J. Paul, AA. Al-Khuraif, S. Vellappally, HS. Halawany, M. Hashim, NB. Abraham, V. Jacob, R. Thavarajah