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2113. Antibodies against b2-glycoprotein I (beta2-GP I) and Their Relationship to Fetoplacental Antigens

Creator:
Lenka Fialová, Malbohan, I., Ludmila Mikulíková, Olga Benešová, and Antonín Zwinger
Format:
print, bez média, and svazek
Type:
article, studie, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Anti-beta2-glycoprotein I, Antiphospholipid antibodies, alpha1-fetoprotein, Human chorionic gonadotrophin, Trophoblast-specific - beta1-glycoprotein, Pregnancy, 14, and 612
Language:
English
Description:
Binding of beta2-GP I to anionic phospholipids is thought to be the major antigen required in the reaction of anticardiolipin antibodies to phospholipids. The aim of this study was to investigate the changes of anti-beta2-GP I IgG during the first and second trimester of pregnancy and the relationship between the levels of anti-beta2-GP I and fetoplacental antigens and the correlation between anti-beta2-GP I IgG and antibodies against oxidized low-density lipoprotein IgG (oLAb) in serum of pregnant women. We determined anticardiolipin antibodies (ACA) IgG and maternal serum levels of alpha1-fetoprotein (AFP), human chorionic gonadotrophin (HCG) and trophoblast-specific beta1-glycoprotein (SP1) in 204 pregnant women in the first and second trimester. From this group we selected 52 serum samples positive for ACA IgG and 16 samples negative for ACA IgG. In the samples of selected patients, the levels of anti-b2-GP I IgG and oLAb IgG were determined. Anti-b2-GP I IgG levels significantly decreased in the second trimester (6.2±9.3 U/ml, mean ± S.D.) in comparison with the first trimester (8.3±10.4 U/ml) (p=0.05). Multiple of median (MoM) AFP correlated negatively but not significantly in the first trimester with anti-b2-GP I (r = -0.261, p = 0.12). In the second trimester this correlation was significantly negative (r = -0.278, p = 0.04). The Spearman correlation coefficients for MoM HCG and anti-beta2-GP I were 0.158 for the first trimester and 0.174 for the second trimester. MoM SP1 also did not correlate significantly with anti-beta2-GP I in both trimesters. The correlation between anti-beta2-GP I IgG and oLAb IgG was not significant (r = -0.06). In the first trimester 40 % serum samples were positive for anti-b2-GP I IgG and negative for oLAb IgG or vice versa, while 60 % samples in the second trimester were positive only for one determined autoantibody. We can conclude that the levels of a, L. Fialová, I. Malbohan, L. Mikulíková, O. Benešová, A. Zwinger., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2114. Antibodies against oxidized LDL - theory and clinical use

Creator:
Anna Steinerová, Racek, J., František Stožický, Tomáš Zima, Lenka Fialová, and Lapin, A.
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, ateroskleróza, oxidační stres, atherosclerosis, oxidative stress, igoxLDL, low density lipoprotein, autoantibodies, 14, and 612
Language:
English
Description:
a1_Modification of low density lipoprotein (LDL) particles due to oxidation, glycation and binding of advanced glycation end-products (AGEs) or malondialdehyde (MDA, a final product of lipid peroxidation) is considered most important in the process of atherogenesis. Oxidatively modified LDL are distinguished by another receptor type, which was discovered on the surface of macrophages and was called the scavenger receptor. Uncontrolled intake of LDL converts macrophages to foam cells; their accumulation under the vascular endothelium is considered as the first stage of atherosclerosis. Oxidation of LDL is a complex process taking place in both the extra- and intracellular space. At the end of this oxidative process, modified LDL particles show chemotactic, cytotoxic and immunogenic properties. Oxidized LDL express a large number of epitopes and cause production of polyclonal autoantibodies against these products, especially against apoB100 modified by MDA and 4-hydroxynonenal. IgoxLDL (antibodies against oxidized LDL) can be demonstrated either directly in intimal lesions or as a component of circulating immune complexes. IgoxLDL do not form a homogeneous group but a varied mixture of antibodies-isoantibodies caused by HDL and LDL polymorphism, antibodies against the lipid phase of LDL and antibodies against modified apoB100 of the immunoglobulin class IgA or IgG. Antibodies against oxLDL were found in many diseases other than atherosclerosis such as diabetes mellitus, renovascular syndrome, uremia, rheumatic fever, morbus Bechtjerev or lupus erythematodes. Newborns have practically the same levels of IgoxLDL as their mothers; however, these values did not differ from those in the healthy population of non-pregnant women of the same age., a2_The decrease in IgoxLDL titer was very slow and lasted many months; that is why this parameter cannot be considered suitable for describing the rapid changes during oxidative stress of the organism. Positive correlation of IgoxLDL with antiphospholipids and other antibodies was repeatedly demonstrated; their determination can thus be used as a marker for the description of total production of autoantibodies in various diseases. The changes and correlations of IgoxLDL, anti-b-2-glycoprotein I IgG and antiphospholipid antibodies support the immunological link between thrombotic and atherosclerotic processes in the human body., A. Steinerová, J. Racek, F. Stožický, T. Zima, L. Fialová, A. Lapin., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2115. Antibodies Against Oxidized Low Density Lipoproteins in Pregnant Women

Creator:
Lenka Fialová, Ludmila Mikulíková, Ivan Matouš Malbohan, Olga Benešová, Stanislav Štípek, Tomáš Zima, and Antonín Zwinger
Format:
print, bez média, and svazek
Type:
article, studie, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Antibodies against oxidized low density lipoproteinsn, Pregnancy, Alpha-1-fetoprotein, Human chorionic gonadotrophin, Trophoblast-specific-beta-1-glycoprotein, 14, and 612
Language:
English
Description:
a1_Oxidized low density lipoproteins (oxLDL) formed in vivo induce a humoral immune response. Oxidative modification of LDL renders it immunogenic and a heterogeneous population of specific anti-oxLDL antibodies is produced. These antibodies could represent a biological marker of oxidative stress and serve as markers of atherosclerosis. Autoantibodies against oxLDL (oLAb) have been detected in human subjects practically of every age. oLAb also appear in the blood of pregnant women. Some studies have shown that the levels of antibodies to oxLDL were elevated in women with established preeclampsia. The present study was aimed to estimate the oLAb IgG levels in the first and second trimester of pregnancy. Furthermore, we estimated the correlation between maternal serum (MS) levels of oLAb and alpha-1-fetoprotein (MS AFP), human chorionic gonadotrophin (MS HCG) and trophoblast-specific-beta-1-glycoprotein (MS SP1), because these proteins are determined as a part of prenatal biochemical screening for fetal congenital abnormalities. Our study deals with the oLAb changes in women with pregnancy-induced hypertension. We also investigated the correlation between oLAb IgG and anticardiolipin antibodies IgG (ACA) in the serum of pregnant women. We examined 40 pregnant women attending Institute for Mother and Child Care for their antenatal care as outpatients. Routine blood samplings between the 9-13th week of pregnancy and 16-18th week of pregnancy were performed as a part of biochemical prenatal screening for fetal congenital abnormalities (Group 1). Their mean age was 27±4.1 years. Furthermore, we examined 26 women in the second or third trimester with pregnancy-induced hypertension (Group 2). Group 2 was compared with 49 pregnant women in the second or third trimester who were normotensive (Group 3)., a2_We used commercial standardized ELISA kits for determination of oLAb IgG, ACA IgG, MS AFP and MS HCG, MS SP1 was analyzed by single radial immunodiffusion. We did not find any differences in the levels of oLAb IgG in the first and second trimester in the women of Group 1. The correlation between oLAb and ACA IgG was not statistically significant (Spearman coefficient r=0.22, p=0.1). The correlation between oLAb IgG with MS AFP, MS HCG and MS SP1 was not statistically significant. Weak negative correlation for AFP and HCG was suggested both in the first and in the second trimester. The levels of oLAb IgG in the group of women with pregnancy-induced hypertension were significantly lower than in the group of normotensive women (348±388 U/ml v.s. 579±400 mU/ml, p<0.01). We can conclude that the levels of oLAb do not differ in the first and second trimester of gravidity. However, we cannot exclude the possible influence of an inverse relationship between oLAb IgG titers and the synthesis of fetoplacental antigens. This finding is important especially in the context of the results of prenatal biochemical screening. Pregnancy-induced hypertension is associated with lower levels of oLAb. Weak cross-reactivity between oLAb and anticardiolipin antibodies may exist but there is a possibility that there are two different populations of antibodies reacting with various antigens., L. Fialová, L. Mikulíková, I. Malbohan, O. Benešová, S. Štípek, T. Zima, A. Zwinger., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2116. Antibodies targeting human endothelin-1 receptors reveal different conformational states in cancer cells

Creator:
Herbet, A., Costa, N., Boquet, D., Hugnot, J.-P., Borrull, A., Couraud, J.-Y., Mabondzo, A., and Leventoux, N.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
fyziologie člověka, human physiology, Monoclonal antibodies, Endothelin-1, Endothelin B receptor, Endothelin A receptor, 14, and 612
Language:
English
Description:
The endothelin axis (endothelins and their receptors) is strongly involved in physiological and pathological processes. ET-1 plays a crucial role in particular in tumor diseases. Endothelin-1 receptors (ETA and ETB) are deregulated and overexpressed in several tumors such as melanoma and glioma. We studied the binding of 24 monoclonal antibodies directed against human ETB receptors (hETB) to different melanoma cell lines. Few of these mAbs bound to all the melanoma cell lines. One of them, rendomab B49, bound to ETB receptors expressed at the surface of human glioma stem cells. More recently, we produced new antibodies directed against human ETA receptor (hETA). Several antibodies have been isolated and have been screened on different tumoral cells lines. As for the mAbs directed against the hETB receptor only some of new antibodies directed against ETA receptor are capable to bind the human tumoral cell lines. Rendomab A63 directed against hETA is one of them. We report the specificity and binding properties of these mAbs and consider their potential use in diagnosis by an in vivo imaging approach., A. Herbet, N. Costa, N. Leventoux, A. Mabondzo, J.-Y. Couraud, A. Borrull, J.-P. Hugnot, D. Boquet., and Seznam literatury
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2117. Anticonvulsant action of GABA-B receptor agonist SKF97541 differs from that of baclofen

Creator:
Pavel Mareš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Experimentální medicína, fyziologie, spazmy, ontogeneze, potkan, physiology, spasms, ontogeny, Rattus norvegicus, GABA-B receptor, agonist, convulsions, 14, and 616-092
Language:
English
Description:
GABA-B receptor agonist SKF97541 exhibits age-dependent anticonvulsant and proconvulsant actions in developing rats. It suppressed tonic phase of generalized seizures induced by pentetrazol in 7-, 12- and 18-day-old rats and increased their latency in 7- and 12-day-old animals. Other results in 18-day-old animals are not so clear. SKF 97541 blocked the appearance of minimal clonic seizures, but tended to decrease latencies of both types of seizures. In addition, it significantly decreases latency of generalized seizures in adult rats. The mixed effects of SKF97541 are in agreement with those of baclofen but there are substantial differences between the actions of these two agonists in individual age groups., P. Mareš., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2118. Antigen presentation and proteome study of exosomes secreted by co-culture of macrophages and Talaromyces marneffei

Creator:
Xie, Zhi, Li, L. L., Wei, G. J., Wang, C., Zhang, D. H., and Wei, L.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Talaromyces marneffei, exosome, antigen presentation, and macrophage
Language:
English
Description:
It is known that intracellular pathogens interact and react with the cellular immune system through exosomes produced by macrophages. This study aimed to determine whether co-culture of macrophages and Talaromyces marneffei induces exosomes and leads to immune responses. T. marneffei was incubated to collect conidia, co-cultured with human macrophages, which then induced exosomes. In cellular experiments, after extraction and purification, the exosomes were then observed by electron microscopy and detected by flow cytometry and mass spectrometry. In animal experiments, flow cytometry and enzyme-linked immunosorbent assay were used to examine whether exosomes were antigenpresenting. The results showed that purified exosomes produced a pro-inflammatory response and stimulated production of TNF-α in non-fungal-treated macrophages. Protein mass spectrometry analysis of exosomes also indicated their potential ability to activate the internal immune response system and the pro-inflammatory response. Translation and ribosomes were the most abundant GO terms in proteins, and the most relevant KEGG pathway was the biosynthesis of secondary metabolites. Furthermore, in vivo experiments revealed that exosomes induced activation of lymphocytes and increased expression of TNF-α and IL-12 in the lung, mediastinum, and spleen area. In conclusion, exosomes can be released by co-culture of T. marneffei and macrophages, having antigen-presenting functions, promoting macrophage inflammation, and initiating adaptive immune responses. These processes are inextricably linked to the translation of secondary metabolites, ribosomes and biosynthesis.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2119. Antika a středověk o čase

Creator:
Macků, Pavel and Novotný, Jan
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
Platón, 427 př. Kr.-347 př. Kr., Aristotelés, 384 př. Kr.-322 př. Kr., svatý, Augustin, 354-430, Tomáš Akvinský, 1225-1274, dějiny fyziky, metafyzika, středověk, čas, history of physics, metaphysics, middle ages, time, 6, and 53
Language:
Czech
Description:
Uvádíme a porovnáváme názory největších myslitelů antiky a středověku - Platóna, Aristotela, Augustina a Tomáše Akvinského - na povahu a vlastnosti času. Všímáme si, jak se jimi formulované názory a problémy odrážejí v současné fyzice., Pavel Macků, Jan Novotný., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2120. Antikvou tištěný český Otčenáš z roku 1555

Creator:
Petr Mašek
Format:
print, text, regular print, bez média, and jiný
Type:
article, zprávy, reports, model:article, and TEXT
Subject:
Staré tisky, Český Krumlov (hrad a zámek)., Knihovna, 16. století, křesťanské modlitby, Modlitba Páně, staré tisky, čeština, antikva, zámecké knihovny, historické knihovní fondy, Christian prayers, Lord's prayer, old prints, Czech language, Roman type, castle libraries, historical book collections, Český Krumlov (Česko), Český Krumlov (Czechia), 12, and 094
Language:
Czech
Description:
Petr Mašek.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

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