Východiska. Trenéři národního týmu vzpírání deklarují, že jejich svěřenci vykazují nedostatky v mentálních dovednostech, což vede k nevyrovnaným výkonům na vrcholných soutěžích. Na základě poznatků o efektivitě mentálního tréninku (MT) v jiných sportech je možné usoudit, že má MT potenciál ve vrcholovém vzpírání. Cíl. Cílem autorů bylo vytvořit a ověřit program založený na technikách mentálního tréninku implementovaných do sportovního tréninku vzpěračů juniorské kategorie České republiky a také reflektovat vliv temperamentových rysů závodníka na implementaci MT program v jeho sportovním tréninku. Metody. Byly provedeny polostrukturované rozhovory se vzpěračem a trenérem před a po realizaci programu mentálního tréninku. Byly použity psychodiagnostické testy – Test pozornosti d2 a TEZADO měřící pozornost resp. dimenze temperamentu, a to za účelem doplnění subjektivních výpovědí. Další metodou doplňující kvantitativní data byly sebehodnotící škály. Výsledky. Z výsledků vyplývá, že některé techniky mentálního tréninku měly pozitivní vliv na úroveň deficitních mentálních dovedností. Tyto mentální dovednosti jsou klíčové při přípravě reprezentanta vzpírání a mohou mít významný vliv na sportovní výkon. Nejvýznamnější rozdíl byl dosažen v dovednosti opětovného zaměření pozornosti a nejmenší v imaginaci. Podle probanda byl vliv temperamentových rysů na program mentálního tréninku významný v souvislosti s opětovným zaměřením pozornosti a zvládáním vzteku a agrese. Závěry. Techniky mentálního tréninku mohou pomoci špičkovým vzpěračům překonat nedostatky v mentálních dovednostech používaných ve sportovní přípravě, což může mít pozitivní vliv na výkonnost v tréninku i soutěžích. Aby byl program úspěšný, je důležité sebepoznání temperamentových rysů. and Background. According to the coaches, the weightlifters of the national team show deficiencies in mental skills, which leads to unbalanced performance at top competitions. Based on the knowledge of the efficacy of mental training (MT) from other sports, it is possible to conclude the potential of the MT in top weightlifting. Objective. The main aim of the authors was to create and verify a program based on mental training techniques implemented in sports training of weightlifter of the junior category of the Czech Republic, and also to reflect the influence of the competitor's temperamental dimensions on the implementation of the MT program in her sports training. Methods. Semi-structured interviews with the athlete and the coach before and after the implementation of the mental training program were conducted. A psychodiagnostic tests – Test of attention d2 and TEZADO measuring attention resp. temperamental dimensions were used in addition to subjective statements. Self-assessing scales were also used to obtain more quantitative data. Results. Results showed that some of the mental training techniques had a positive effect on deficient mental skills. These mental skills are crucial in the preparation of the weightlifting representative and can have a significant influence on a sports athlete's performance. The most significant difference was achieved in the skill of refocusing and the smallest difference in imagination. According to the subject, the influence of the temperamental dimensions on the mental training program had a significant impact in connection with the refocusing and anger coping. Conclusion. Mental training techniques can help top weightlifters to overcome the mental skills deficiencies used in their sports training, which may have a positive influence on performance in both training and competitions. Self-knowledge of the temperamental dimensions is significant to the success of the program.
Granulosa cells (GCs) are somatic cells essential for establishing and maintaining bi-directional communication with the oocytes. This connection has a profound importance for the delivery of energy substrates, structural components and ions to the maturing oocyte through gap junctions. Cumulus cells, group of closely associated GCs, surround the oocyte and can diminished the effect of harmful environmental insults. Both GCs and oocytes prefer different energy substrates in their cellular metabolism: GCs are more glycolytic, whereas oocytes rely more on oxidative phosphorylation pathway. The interconnection of these cells is emphasized by the fact that GCs supply oocytes with intermediates produced in glycolysis. The number of GCs surrounding the oocyte and their age affect the energy status of oocytes. This review summarises available studies collaboration of cellular types in the ovarian follicle from the point of view of energy metabolism, signaling and protection of toxic insults. A deeper knowledge of the underlying mechanisms is crucial for better methods to prevent and treat infertility and to improve the technology of in vitro fertilization.
Methamphetamine (MA), as a psychostimulant drug that crosses
the placental barrier, may disrupt the development of social play.
The present study aims to examine the effect of prenatal MA
(5 mg/kg) exposure during the first (gestational day (GD) 1-11)
or second (GD 12–22) halves of prenatal development of rats on
social play behavior. To investigate an acute effect of MA on
social play in adulthood, juvenile rats were exposed to a dose of
1 mg/kg MA or saline on the test day and tested for social play
for 15 min. Prenatal exposure to MA during GD 1–11 increased
social play behavior during 5-10 min interval of the test in males
but not females. Prenatal MA during GD 12–22 did not influence
social play in males nor females. However, social play occurred to
a greater extent in GD 12–22 groups compared with GD 1–11.
Acute exposure to MA eliminated playful behavior in all groups
and decreased social exploration in GD 1–11. Our results suggest
that manipulation of prenatal development during the first half of
the gestational period has a greater impact on social play
behavior than during the second half.
Midazolam is a short acting sedative with small number of adverse effects. Administered orally, it is currently the most common form of conscious sedation in children. The objective of this paper is to describe effect of midazolam administered to children during dental treatment on their vital signs, and to monitor changes in children’s behavior. We described values of vital signs and behavior in 418 sedations conducted in 272 children between 1-12 years of age. To achieve the following results, we used data from 272 all first-time sedations. After administration of midazolam arterial blood pressure and blood oxygen saturation decreased by values which were not clinically significant. The heart rate increased, with values staying within the limits of physiological range. The speed of onset of midazolam’s clinical effects depends on age and dose. The lower age and dose correlated with the higher behavior score. The effectiveness of midazolam treatment is 97.8 %. Unwillingness of child to receive midazolam is predictor for disruptive behavior during sedation. 1.8 % of all sedation cases showed paradoxical reactions. The administration of midazolam in dose of 0.5 mg per 1 kg of child’s body weight is safe and could be recommended for dental treatment in pediatric dentistry., Jana Vasakova, Jana Duskova, Jitka Lunackova, Klara Drapalova, Lucie Zuzankova, Luboslav Starka, Michaela Duskova, Zdenek Broukal., and Obsahuje bibliografii
The main goal of our prospective randomized study was
comparing compare the effectiveness of ventilation control
method „Automatic proportional minute ventilation (APMV)
“versus manually set pressure control ventilation modes in
relationship to lung mechanics and gas exchange. 80 patients
undergoing coronary artery bypass grafting (CABG) were
randomized into 2 groups. 40 patients in the first group No. 1
(APMV group) were ventilated with pressure control (PCV) or
pressure support ventilation (PSV) mode with APMV control. The
other 40 patients (control group No.2) were ventilated with
synchronized intermittent mandatory ventilation (SIMV-p) or
pressure control modes (PCV) without APMV. Ventilation control
with APMV was able to maintain minute ventilation more
precisely in comparison with manual control (p<0.01), similarly
deviations of ETCO2 were significantly lower (p<0.01). The
number of manual corrections of ventilation settings was
significantly lower when APMV was used (p<0.01). The
differences in lung mechanics and hemodynamics were not
statistically significant. Ventilation using APMV is more precise in
maintaining minute ventilation and gas exchange compared with
manual settings. It required less staff intervention, while
respiratory system mechanics and hemodynamics are
comparable. APMV showed as effective and safe method
applicable on top of all pressure control ventilation modes.
Circulating miRNAs have been proposed as the effective diagnostic biomarkers for muscular fibrosis-associated diseases. However, circulating biomarkers for early diagnosis of contracture muscles are limited in gluteal muscle contracture (GMC) patients. Here we sought to explore the abnormally expressed miRNAs in plasma and contraction bands of GMC patients. The results showed miR-29a-3p expression in plasma and contraction bands tissue was significantly reduced in GMC patients compared with normal control. Cell viability and levels of proliferation-associated protein cyclin D1 and cyclindependent-kinase 2 (CDK2) were powerfully inhibited by miR-29a mimics and enhanced by miR-29a inhibitor compared with negative control. Furthermore, miR-29a mimics effectively impeded, while miR-29a inhibitor enhanced the expression of collagen I and collagen III, followed by the secretion of transforming growth factor β1 (TGF-β1), TGF-β3 and connective tissue growth factor (CTGF) in primary human contraction bands (CB) fibroblasts. The miR-29a-3p negatively regulated the expression of TGF-β1 through binding to the 3′ UTR region of SERPINH1 (encoding heat shock protein HSP47), but had no effect on Smad2 activity. The miR-29a-3p was inversely correlated with HSP47 in contraction bands tissue from GMC patients. Collectively, miR-29a was notably depressed and regulated cell viability and fibrosis by directly targeting HSP47 in GMC, which suggest that circulating miR-29a might be a potential biomarker for early diagnosis and provides a novel therapeutic target for GMC.
Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects.
We studied the effects of GABA receptor agonists microinjections
in medullary raphé on the mechanically induced tracheobronchial
cough response in anesthetized, unparalyzed, spontaneously
breathing cats. The results suggest that GABA-ergic inhibition
significantly contributes to the regulation of cough reflex by
action of both GABAA and GABAB receptors. The data are
consistent with inhomogeneous occurrence of GABA-ergic
neurons in medullary raphé and their different involvement in the
cough reflex control. Cells within rostral nucleus raphé obscurus
with dominant role of GABAA receptors and neurons of rostral
nucleus raphé pallidus and caudal nucleus raphé magnus with
dominant role of GABAB receptors participate in regulation of
cough expiratory efforts. These cough control elements are
distinct from cough gating mechanism. GABA-ergic inhibition in
the raphé caudal to obex had insignificant effect on cough.
Contradictory findings for GABA, muscimol and baclofen
administration in medullary raphé suggest involvement of
coordinated activity of GABA on multiple receptors affecting
raphé neurons and/or the local neuronal circuits in the raphé
modulating cough motor drive.