We studied hsBAFF activity in in vitro mouse splenic B cells. hsBAFF effects on intracellular free Ca 2+ concentration ([Ca 2+ ] i ) were assayed, using a laser scanning confocal microscope with fluorescent probe, Fluo-3/AM. We showed that treatment of B cells with 0.5-5 μ g/ml hsBAFF resulted in significantly higher [Ca 2+ ] i levels in a dose-dependent fashion at 12 and 24 h, respectively (p<0.05 or p<0.01 vs. control). Furthermore, we noticed that 2.5 μ g/ml hsBAFF-treated cells were significantly resistant to decrease of cellular viability induced by thapsigargin (Tg), an endoplasmic reticulum (ER) Ca 2+ -ATPase inhibitor (p<0.05 hsBAFF plus Tg group vs. Tg group). Thus hsBAFF may promote B cell survival by direct upregulation of [Ca 2+ ] i physiological homeostasis contri buting to prevention of [Ca 2+ ] i dysfunction. Using immunocytochemistry and Western blot analysis, we found that the activation of ERK1/2 due to hsBAFF was triggered by a [Ca 2+ ] i -dependent pathway, leading to elevation of B cell proliferation. This is supported by the findings that intracellular Ca 2+ chelator BAPTA/AM attenuated phosphorylated ERK1/2 expression and cell proliferation in hsBAFF-stimulated B cells. hsBAFF-stimulated B cell proliferation was obviously reduced by mitogen extracellular kinase 1/2 (MEK1/2, upstream of ERK1/2) inhibitor U0126. Taken together, the main finding of this study is that hsBAFF elicits higher but homeostatic [Ca 2+ ] i levels, which regulates ERK1/2 activity and cell proliferation in in vitro B cells., J. Q. Liang, W. Zhang, L. Wen, W. Gao, S. Q. Zhang, L. Chen., and Obsahuje bibliografii
Studie Martina Voříška je věnována hudebnímu životu na zámku v Českém Krumlově v roce 1780. Autor jej sleduje prostřednictvím neobvyklé události. 12. srpna 1780 totiž byla vykradena knížecí pokladna, z níž se ztratilo více než 950 zlatých. V té době ji měl na starosti kancelista a dvorní hudební ředitel Antonín Jan Jüngling. Aby se zbavil z podezření, že byl pachatelem tohoto činu, vytvořil detailní soupis své denní činnosti, díky níž tak zcela mimoděk vznikl unikátní dokument reflektující činnost schwarzenberské kapely v té době. Výňatky z tohoto dokumentu, jež se týkají hudby, jsou citovány v příloze., The article is based on a lengthy report by Anton Johann Jüngling on his own activities on 12 August 1780; Jüngling was at the time the Schwarzenbergs’ music director and also a highly placed official. This unique historical source affords a very detailed look at Jüngling’s daily life as a person and a professional, and in combination with other sources, it also enables the creating of a quite detailed picture of the musical events at the Schwarzenberg court over a period of just under two weeks, when the Schwarzenbergs were staying in Český Krumlov with their guests. The musical activities included theatrical performances, a ball, a private concert with repertoire consisting of orchestral arrangements of music from singspiels currently being performed (Die schöne Schusterin and Die abgeredte Zauberey), and accompaniment for worship services. The study documents that the musicians at the princely court were able to secure the music for most of these occasions, and that they were supplemented or substituted for as needed by hired musicians from the town or the military., Martin Voříšek., Rubrika: Studie, and Anglické resumé na s. 141-142, anglický abstrakt na s. 117.
Studie Jiřího Sehnala se zabývá hudebním životem v augustiniánském klášteře u Všech svatých v Olomouci v době baroka a osvícenství., Jiří Sehnal., Rubrika: Studie, and Anglické resumé na s. 271, anglický abstrakt na s. 245.
This study is dedicated to a collection of compositions titled Hudební album (A Musical Album), volumes of which were issued in print from 1891 until 1896 by the Prague publisher Fr. A. Urbanek. The collection focused primarily on piano compositions by contemporary Bohemian composers. The first part of the study is devoted principally to the contents of the collection and to the circumstances of its publishing, then the second part examines the printing of compositions by A. Dvořák (All through the night a bird will sing from the song cycle Evening Songs, op. 31, Furiant, op. 12/2, and Dumka, op. 12/1) in Hudební album in versions that differ from the first editions published by Fr. A. Urbanek. This fact has not yet been researched by Dvořák scholars., Helena Matějčková., and Anglické resumé na s. 333.
Příspěvek Ondřeje Maňoura je zprávou z výroční konference České společnosti pro hudební vědu, která se uskutečnila v Praze ve dnech 25. až 26. listopadu 2011., Ondřej Maňour., Rubrika: Konference, and Cizojazyčné resumé není.