The long-term electrocardiographic recording with retrospective evaluation (Holter system) has been widely used not only in cardiology, but also in other disciplines of internal medicine and in pharmaceutical research. The Holter system can be used in mini-pig, sheep, dog, cat, rabbit, ferret, and rat. In this paper hardware, software, and anesthesia requirements are summarized with respect to the experimental work with various species. As the Holter systems work in bipolar mode, the use of bipolar leads in sagittal and transversal planes has been proved to be the most appropriate because of large amplitude of QRS complex and uncomplicated consequent automatic analysis of the record. In conclusion, Holter electrocardiography represents a simple and applicable method for monitoring the electrical activity of the heart in small animals’ experimental studies., P. Scheer ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Combustion of coal with limestone (a) and co-combustion of coal with limestone with wood, sewage sludge, soap and PTP (b) were performed in CFB. Inorganic matter composition and properties of all input materials were characterized. For this, chemical and coal petrography analyses of solid samples as well as their leaching tests, unburned carbon, and other properties were determined. There are not striking differences in composition of bottom and fly ashes from combustion/co-combustion data. In fly ash samples higher concentrations of Hg (about 2 ppm), As (about 800 ppm) and chlorine (about 400 ppm) are present than they are in bottom ash (0.002 ppm Hg, 250 ppm As, and 220 ppm Cl). Also relating concentrations of above elements determined in emissions are very similar for combustion/co-combustion regimes (about 0.010 mg.m-3 Hg, 0.027 mg.m-3 As and 24 mg.m-3 Cl). Content of unburned carbon in BA is below 2 wt.% and in FA below 0.6 wt.% in all combustion/co-combustion data. In FA samples the concentration of Hg, As, Se, etc. increases with increasing unburned carbon content, i.e. with decreasing grain size of FA. No significant differences in composition of emissions, bottom and fly ashes as well as in composition of water leachates were found from the combustion regimes I, II, III and IV., Martin Stach, Zdeněk Klika, Martina Nováčková and Václav Roubíček., and Obsahuje bibliografii
The functional aversive stimulus properties of several IP doses of (±)-amphetamine (1.25-10 mg.kg-1), 2-phenylethylamine (PEA, 2.5-10 mg.kg-1, following inhibition of monoamine oxidase with pargyline 50 mg.kg-1) and phenylethanolamine (6.25-50 mg.kg 1) were measured with the conditioned taste aversion (CTA) paradigm. A two bottle choice procedure was used, water vs. 0.1 % saccharin with one conditioning trial and three retention trials. (±)-Amphetamine and phenylethanolamine induced a significant conditioned taste aversion but PEA did not. (±)-Amphetamine and PEA increased spontaneous locomotor activity but phenylethanolamine had no effects on this measure. Measurement of whole brain levels of these drugs revealed that the peak brain elevation of PEA occurred at approximately 10 min whereas the peak elevations of (±)-amphetamine and phenylethanolamine occurred at approximately 20 min. The present failure of PEA to elicit conditioned taste aversion learning is consistent with previous reports for this compound. The differential functional aversive stimulus effects of these three compounds are surprising since they exhibit similar discriminative stimulus properties and both (±)-amphetamine and PEA are self-administered by laboratory animals. The present data suggest that time to maximal brain concentrations following peripheral injection may be a determinant of the aversive stimulus properties of PEA derivatives., A.J. Greenshaw, S. Turkish, B.A. Davis., and Obsahuje bibliografii
A technique based on the analysis of banding patterns obtained by SDS-PAGE Western-blotting of an oocyst wall antigen obtained from faeces has been evaluated to subtype Cryptosporidium parvum Tyzzer, 1912. This technique appears to have sufficient stability to recognise multiple types of this parasite. A similar Western-blotting technique has also been used to assess antibody responses to cryptosporidial antigens in human sera. Two systems were developed: one against three antigens of apparent molecular weights 6, 14 and 17 kDa; the second against oocyst wall antigens of apparent molecular weights 57, 69, 75, 89, 128, 151 and 173 kDa. Antibodies to three antigens of apparent molecular weights 6, 14 and 17 kDa were most successful as diagnostic markers in that they were found in >88% of convalescent phase sera from confirmed cryptosporidiosis patients and were uncommon (>7%) in control subjects. Faecal samples from human and animal sporadic cases yielded a wide range of cryptosporidial antigen banding patterns. Samples from patients in a water-borne outbreak in South Devon (England) in 1995 also yielded a wide range of banding patterns including members within individual household family groups. These results are in contrast with those from samples collected from other defined geographical areas, including some from a second water-borne outbreak where much more homogeneous banding patterns were obtained. Sera collected for other purposes from apparently uninfected individuals 9 months after the South Devon 1995 outbreak were examined. Antibodies to the three antigens of molecular weights 6, 14 and 17 kDa were detected in 32-49% of individuals resident in the outbreak water supply area, and in 15-21%) of those resident in an adjacent water supply area. The significance of these findings is discussed in relation to data obtained from epidemiological field studies.
The reactivity of a char depends very on the parent coal. Much information about correlation between properties of coal and reactivity of chars is lost by using only standard methods for a large dataset evaluation. In this research a set of 8 coals has been investigated by thermal analysis and reactivity of obtained chars was analyzed as a function of properties of parent coal properties using Canonical correlation analysis. The reactivity of chars was determined by thermogravimetric analysis of nonisothermal combustion in oxygen. It can be stated that methods of multivariate data analysis are useful tools for the interpretation of coal chars reactivity data., Mariusz Minkina, Elwira Zajusz-Zubek and Andrzej Mianowski., and Obsahuje bibliografii
The paper discusses a problem of salt structures recent vertical movements that are observed by geodetic surveys on the example of salt dome in Inowroclaw. Presented survey results were carried by several years with use of precise levelling and GPS survey techniques. The new surveys were involved in 2002 that are carried on established network points and benchmarks of former existed country levelling network. Comp arative analysis of obtained displacement results derived by different methods is discussed as well. The effects of the an alysis are conclusions providing the usefulness of GPS techniques in the presented research. Other conclusions derived from carried observation deal with feat ures of determined vertical displacements., Zbigniew Szczerbowski., and Obsahuje bibliografické odkazy
The primary aim was to determine frequencies of mutations related to risk of venous thrombosis in healthy Caucasians in Central Bohemia. In a cohort of 1527 healthy individuals the frequency of risk alleles for the mutations FV Leiden and FII 20210G>A was 4.5 % and 1.3 %, respectively. Frequency of 4G PAI-1 allele was 55.5 %. Genotype frequencies were: GG 91.03 %, GA 8.91 %, an d AA 0.07 % for FV Leiden; GG 97.45 %, GA 2.49 %, and AA 0.07 % for FII 20210G>A; 4G/4G 30.26 %, 4G/5G 50.56 %, and 5G/5G 19.19 % for PAI-1. Frequency of the risk allele A in polymorphism SERPINC1 (IVS +141G >A) was 11.3 %, and frequencies of genotypes were as follows: GG 78.36 %, GA 20.66 %, and AA 0.98 %. Frequency of the risk allele T for polymorphism GP6 13254T>C was 87.7 %, and frequencies of genotypes were as follows: TT 77.14 %, TC 21.15 %, and CC 1.70 %. Frequency of the risk allele A in polymorphism CYP4V2 (Lys259Gln ) was 65.2 %, and frequencies of genotypes were: CC 12.25 %, CA 45.12 %, and AA 42.63 %. All observed genotypes and alleles frequencies were without gender differences. Their occurrences confirm a relatively high prevalence of hereditary thrombophilia predisposition in the Czech Republic., T. Kvasnička ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
It is well known that the training level of a muscle belongs to the parameters that affect the H-reflex response amplitude. The aim of this study was to investigate the effects of training type on H- and T-reflex response parameters. For this purpose, 20 long-distance athletes (group I, test group), 18 short-distance athletes (group II, test group) and 20 non-trained subjects (group III, control group) were involved in this study in which the H- and T-reflex amplitude and latency values were measured. The H-reflex amplitude and latency values found in groups I, II and III were 3.64±0.28 mV and 26.88±1.45 ms, 3.17±0.26 mV and 26.19±1.89 ms, and 6.07±0.34 mV and 26.77±1.32 ms, respectively. The T-reflex amplitude and latency values of the groups I, II and III were 3.30±0.18 mV and 32.01±1.02 ms, 3.11±0.20 mV and 31.47±1.16 ms, 4.24±0.21 mV and 31.47±1.16 ms, respectively. There was no statistically significant difference between the groups with respect to latencies of H- and T-reflexes (p>0.05). In both test groups, the amplitudes of the H-reflex and T-reflex were significantly smaller than the control group (p<0.05). The results of this study suggest that training of muscles affect the H- and T-reflex response parameters., R. Ozmerdivenli, S. Bulut, T. Urat, A. Ayar., and Obsahuje bibliografii
The ultrastructural cytology and reproduction of Amphiacantha longa Caullery et Mesnil, 1914 is described. Mcrogonial reproduction was not observed. The sporogony follows two lines: free disporoblastic. and enveloped, polysporoblastic, involving sporoblast mother cells. The enveloped sporogony is endogenous in spore sacs of sporont origin, daughter cells are formed by vacuolation. Probably all stages have coupled nuclei. Both free and enveloped spores are equipped with an extrusion apparatus composed of a flat polar sac, a straight polar filament of manubrium type, and a posterior globular appendix. Manubrium and appendix are enclosed in a membraneous coat. Circular elements of coat material occur in the proximity of the extrusion apparatus. The membraneous coat and the surface layer of the manubrium penetrate the polar sac. The extrusion apparatus is located at the wide pole of the spore, the nuclei at the narrower pole. Hosts are gregarines of the genus Lecudina Mingazzini, living in the gut of the polychaete Lumbrinereis fragilis (O. F. Muller). The cytology and reproduction are discussed and compared to other genera of metchnikovellideans, to the chytridiopsid genera, and to microsporidia expressing the typical cytology for the group. Metchnikovellideans and chytridiopsids exhibit cytological and reproductive similarities. The species is redescribed, the diagnosis of the genus Amphiacantha Caullery et Mesnil, 1914 is emended, and the new family Amphiacanthidae, comprising the genera Amphiacantha and Amphiamblys Caullery et Mesnil, 1914, is established.