Ninety four aquarium fishes were screened for the presence of amoebae in their internal organs. Five specimens of Ca-rassius auratus (L.) and one specimen of Xiphophorus hetleri Heckel were positive. Of the three strains which were isolated from C. auratus, successfully cloned and cultivated, one was identified as Vannella platypodia (Gläser, 1912) Page, 1976 and two strains as Rosculus ithacus Hawes, 1963. Both species are reported for the first time from organs of fish. None of them could be identified with the amoeba-like agent of goldfish granulomas described here.
From November 1997 to June 1998, 3,118 specimens of Echinogammarus stammeri (Karaman, 1931) (Amphipoda) were collected from the River Brenta (Northern Italy) and examined for larval helminths. Larvae of Polymorphus minuius (Goeze, 1782) singly infected the hemocoel of 23 (0.74%) crustaceans; all these larvae were cystacanth stages. This is the first record of Polymorphus minuius in E. stammeri. Some cystacanths had their forebody and hindbody fully inverted. Parasites were bright orange in colour and each was surrounded by a thin acellular envelope. This envelope likely protects the developing parasite larva from cellular responses of the amphipod. Hemocytes were seen adherent to the outer surface of the envelope. The sex ratio among the parasitised E. stammeri was almost 1:1. All Polymorphus minutus larvae were central in the amphipod body, made intimate contact with host internal organs, and frequently induced a marked displacement of them. None of the infected females of E. stammeri. carried eggs or juveniles in their brood pouch. In five hosts, Polymorphus minuius co-occurrcd with the cystacanth of another acanlhocephalan, Pomphorhynchus laevis (Millier, 1776), a parasite offish.
The object of the research was to investigate the spectral properties of Rayleigh-type surface waves, generated by shot-hole explosions during seismic refraction experiments which were carried out in the area of the Bohemian Massif and West Carpathians. The records of displacement amplitudes were spectrally analyzed and prevailing frequency fp, relative Δfr and absolute widths of the spectra Δfa were chosen as essential parameters. Whilst the prevailing frequencies were recorded within the interval f ÷ 0.80 - 3.70 Hz at the site of the observations, situated on the territory of the Bohemian Massif, the respecti ve frequency range f ÷ 0.80 - 2.6 Hz was found in the West Carpathians. Some functional dependences of the spectral amplitude parameters on epicentral distance were observed and regularities of their decrease were defined. Moreover, the influence of local seismogeological conditions at the shot point as well as at the site of observation occurred., Karel Holub., and Obsahuje bibliografické odkazy
Classical biological control is an important means of managing the increasing threat of invasive plants. It constitutes the introduction of natural enemies from the native range of the target plant into the invaded area. This method may be the only cost-effective solution to control the rapidly expanding common ragweed, Ambrosia artemisiifolia, in non-crop habitats in Europe. Therefore, candidate biocontrol agents urgently need to be assessed for their suitability for ragweed control in Europe. A previous literature review prioritized the host-specific leaf beetle Ophraella slobodkini as a candidate agent for ragweed control in Europe, whereas it rejected its oligophagous congener O. communa. Meanwhile, O. communa was accidentally introduced and became established south of the European Alps, and we show here that it is expanding its European range. We then present a short version of the traditional pre-release risk-benefit assessment for these two candidate agents to facilitate fast decision-making about further research efforts. We selected two complementary tests that can be conducted relatively rapidly and inform about essential risks and benefits. We conducted a comparative no-choice juvenile performance assay using leaves of ragweed and sunflower, the most important non-target plant, in Petri dishes in climatic conditions similar to that in the current European range of O. communa. This informs on the fundamental host range and potential for increasing abundance on these host plants. The results confirm that O. slobodkini does not survive on, and is hence unlikely to cause severe damage to sunflower, while O. communa can survive but develops more slowly on sunflower than on ragweed. In parallel, our species distribution models predict no suitable area for the establishment of O. slobodkini in Europe, while O. communa is likely to expand its current range to include a maximum of 18% of the European ragweed distribution. Based on this early assessment, the prioritization and further assessment of O. slobodkini seem unwarranted whereas the results urgently advocate further risk-benefit analysis of O. communa. Having revealed that most of the European area colonized by ragweed is unlikely to be suitable for these species of Ophraella we suggest the use of such relatively short and cheap preliminary assessment to prioritise other candidate agents or strains for these areas., Suzanne T. E. Lommen, Emilien F. Jolidon, Yan Sun, José I. Bustamante Eduardo, Heinz Müller-Schärer., and Obsahuje bibliografii
5-hydroxytryptamine (5-HT) is involved in the stress-induced alteration of colonic functions, specifically motility and secretion, but its precise mechanisms of regulation remain unclear. In the present study, we have investigated the effects of 5-HT on rat colonic mucosal secretion after acute water immersion restraint stress, as well as the underlying mechanism of this phenomenon, using short circuit current recording (ISC), real-time polymerase chain reaction, Western blot analysis, and enzyme-linked immunosorbance assays. After 2 h of water immersion restraint stress, the baseline ISC and 5-HT-induced ISC responses of the colonic mucosa were significantly increased. Pretreatment with selective 5-HT4 receptor antagonist, SB204070, inhibited the 5-HT-induced colonic ISC response by 96 % in normal rats and 91.2 % in acute-stress rats. However, pretreatment with the selective antagonist of 5-HT3 receptor, MDL72222 or Y-25130, had no obvious effect on 5-HT-induced ISC responses under either set of conditions. Total protein expression of both the mucosal 5-HT3 receptors and the 5-HT4 receptors underwent no significant changes following acute stress. Both colonic basal cAMP levels and foskolin-induced ISC responses were significantly enhanced in acute stress rats. 5-HT significantly enhanced the intracellular cAMP level via 5-HT4 receptors in the colonic mucosa from both control and stressed animals, and 5-HT-induced cAMP increase in stressed rats was not more than that in control rats. Taken together, the present results indicate that acute water immersion restraint stress enhances colonic secretory responses to 5-HT in rats, a process in which increased cellular cAMP accumulation is involved., Y. Li, L. S. Li, X. L. Zhang, Y. Zhang, J. D. Xu, J. X. Zhu., and Obsahuje bibliografii
Vitamin D is synthesised in the skin during exposure to sunlight and its fundamental roles are the regulation of calcium and phosphate metabolism and bone mineralisation. The aim of our study was to evaluate serum levels of 25-hydroxyvitamin D3 , PTH and bone turnover markers (P1NP, OC, β -CTx, OC/ β -CTx) and the intake of calcium and vitamin D in Polish Professional Football League (Ekstraklasa) players and in young men with a low level of physical activity. Fifty healthy men aged 19 to 34 years were included in the study. We showed that 25(OH)D3 and P1NP levels and OC/ β -CTx were higher in the group of professional football players than in the group of phys ically inactive men. The daily vitamin D and calcium intake in th e group of professional football players was also higher. We showed a significant relationship between 25(OH)D3 levels and body mass, body cell mass, total body water, fat-free mass, muscle mass, vitamin D and calcium intake. Optimum 25(OH)D3 levels were observed in a mere 16.7 % of the football players and vitamin D deficiency was observed in the physically inactive men. The level of physical activity, body composition, calcium and vitamin D intake and the duration of exposure to sunlight may significantly affect serum levels of 25(OH)D3 ., K. Solarz ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR) play crucial role in the regulation of drug metabolizing enzymes and in many essential physiological processes. Cellular signaling by these receptors shares several functional and regulatory features. Here we investigated regulatory cross-talk between these two receptors. Human hepatoma cells (HepG2) were the model of choice. We analyzed the effects of dexamethasone (DEX) and dioxin (TCDD) on i) expression of AhR and GRα mRNAs; ii) levels of AhR and GR proteins; iii) transcriptional activities of AhR and GR in reporter assays; iv) 7-ethoxyresorufin-O-deethylase activity (EROD). We found that both DEX and TCDD affected AhR and GR mRNAs expression, proteins levels and transcriptional activities in HepG2 cells. These effects on cellular signaling by AhR and GR comprised up-/down-regulation of gene expression and ligand-dependent protein degradation. We conclude that interactive regulatory cross-talk between GR and AhR receptors in HepG2 cells defines possible implications in physiology and drug metabolism. Future research should be focused on the investigation of AhR-GR cross-talk in various normal human cells and tissues both in vitro and in vivo., Z. Dvořák, R. Vrzal, P. Pávek, J. Ulrichová., and Obsahuje bibliografii a bibliigrafické odkazy
Peritoneal dialysis (PD) is a well established method of depuration in uremic patients. Standard dialysis solutions currently in use are not biocompatible with the peritoneal membrane. Studying effects of dialysate on peritoneal membrane in humans is still a challenge. There is no consensus on the ideal experimental model so far. We, therefore, wanted to develop a new experimental non-uremic rabbit model of peritoneal dialysis, which would be practical, easy to conduct, not too costly, and convenient to investigate the long-term effect of dialysis fluids. The study was done on 17 healthy Chinchilla male and female rabbits, anesthetized with Thiopental in a dose of 0.5 mg/kg body mass. A catheter, specially made from Tro-soluset (Troge Medical GMBH, Hamburg, Germany) infusion system, was then surgically inserted and tunneled from animals' abdomen to their neck. The planned experimental procedure was 4 weeks of peritoneal dialysate instillation. The presented non-uremic rabbit model of peritoneal dialysis is relatively inexpensive, does not require sophisticated technology and was well tolerated by the animals. Complications such as peritonitis, dialysis fluid leakage, constipation and catheter obstruction were negligible. This model is reproducible and can be used to analyze the effects of different dialysis solutions on the rabbit peritoneal membrane., S. Zunic-Bozinovski, Z. Lausevic, S. Krstic, N. Jovanovic, J. Trbojevic-Stankovic, B. Stojimirovic., and Obsahuje bibliografii a bibliografické odkazy