As a common waterfront and wet environment tree species, Salix babylonica shows a great potential for restoration of contaminated water or soil environments, such as phenol-polluted water. However, studies on such remediation effects have not been carried out yet. The objective of this study was to investigate the effects of phenols on photosynthesis of S. babylonica. Photosynthetic and chlorophyll fluorescence parameters of S. babylonica cuttings were determined in hydroponic experiment, where six phenol concentrations was used (0, 50, 100, 200, 400, and 800 mg L-1). Phenol presence inhibited photosynthesis of S. babylonica significantly, as the net photosynthetic rate (PN),
light-saturated net photosynthetic rate, apparent quantum yield, maximal quantum yield of PSII photochemistry, and effective quantum yield of PSII photochemistry declined significantly. The higher the concentration of phenol solution, the greater inhibition of photosynthesis occurred. Our data indicated that nonstomatal limitation was responsible for the reduction of PN. S. babylonica should be used to remediate phenol-contaminated water, when the concentration of phenol solution is lower than 200 mg L-1. Otherwise, the efficiency of photosynthesis of S. babylonica would decrease markedly. However, further study is needed to determine the maximum concentration of phenol that S. babylonica can tolerate to maintain normal photosynthetic activity., H. Li, G. C. Zhang, H. C. Xie, K. Li, S. Y. Zhang., and Obsahuje seznam literatury
The long-term electrocardiographic recording with retrospective evaluation (Holter system) has been widely used not only in cardiology, but also in other disciplines of internal medicine and in pharmaceutical research. The Holter system can be used in mini-pig, sheep, dog, cat, rabbit, ferret, and rat. In this paper hardware, software, and anesthesia requirements are summarized with respect to the experimental work with various species. As the Holter systems work in bipolar mode, the use of bipolar leads in sagittal and transversal planes has been proved to be the most appropriate because of large amplitude of QRS complex and uncomplicated consequent automatic analysis of the record. In conclusion, Holter electrocardiography represents a simple and applicable method for monitoring the electrical activity of the heart in small animals’ experimental studies., P. Scheer ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Combustion of coal with limestone (a) and co-combustion of coal with limestone with wood, sewage sludge, soap and PTP (b) were performed in CFB. Inorganic matter composition and properties of all input materials were characterized. For this, chemical and coal petrography analyses of solid samples as well as their leaching tests, unburned carbon, and other properties were determined. There are not striking differences in composition of bottom and fly ashes from combustion/co-combustion data. In fly ash samples higher concentrations of Hg (about 2 ppm), As (about 800 ppm) and chlorine (about 400 ppm) are present than they are in bottom ash (0.002 ppm Hg, 250 ppm As, and 220 ppm Cl). Also relating concentrations of above elements determined in emissions are very similar for combustion/co-combustion regimes (about 0.010 mg.m-3 Hg, 0.027 mg.m-3 As and 24 mg.m-3 Cl). Content of unburned carbon in BA is below 2 wt.% and in FA below 0.6 wt.% in all combustion/co-combustion data. In FA samples the concentration of Hg, As, Se, etc. increases with increasing unburned carbon content, i.e. with decreasing grain size of FA. No significant differences in composition of emissions, bottom and fly ashes as well as in composition of water leachates were found from the combustion regimes I, II, III and IV., Martin Stach, Zdeněk Klika, Martina Nováčková and Václav Roubíček., and Obsahuje bibliografii
The functional aversive stimulus properties of several IP doses of (±)-amphetamine (1.25-10 mg.kg-1), 2-phenylethylamine (PEA, 2.5-10 mg.kg-1, following inhibition of monoamine oxidase with pargyline 50 mg.kg-1) and phenylethanolamine (6.25-50 mg.kg 1) were measured with the conditioned taste aversion (CTA) paradigm. A two bottle choice procedure was used, water vs. 0.1 % saccharin with one conditioning trial and three retention trials. (±)-Amphetamine and phenylethanolamine induced a significant conditioned taste aversion but PEA did not. (±)-Amphetamine and PEA increased spontaneous locomotor activity but phenylethanolamine had no effects on this measure. Measurement of whole brain levels of these drugs revealed that the peak brain elevation of PEA occurred at approximately 10 min whereas the peak elevations of (±)-amphetamine and phenylethanolamine occurred at approximately 20 min. The present failure of PEA to elicit conditioned taste aversion learning is consistent with previous reports for this compound. The differential functional aversive stimulus effects of these three compounds are surprising since they exhibit similar discriminative stimulus properties and both (±)-amphetamine and PEA are self-administered by laboratory animals. The present data suggest that time to maximal brain concentrations following peripheral injection may be a determinant of the aversive stimulus properties of PEA derivatives., A.J. Greenshaw, S. Turkish, B.A. Davis., and Obsahuje bibliografii
A technique based on the analysis of banding patterns obtained by SDS-PAGE Western-blotting of an oocyst wall antigen obtained from faeces has been evaluated to subtype Cryptosporidium parvum Tyzzer, 1912. This technique appears to have sufficient stability to recognise multiple types of this parasite. A similar Western-blotting technique has also been used to assess antibody responses to cryptosporidial antigens in human sera. Two systems were developed: one against three antigens of apparent molecular weights 6, 14 and 17 kDa; the second against oocyst wall antigens of apparent molecular weights 57, 69, 75, 89, 128, 151 and 173 kDa. Antibodies to three antigens of apparent molecular weights 6, 14 and 17 kDa were most successful as diagnostic markers in that they were found in >88% of convalescent phase sera from confirmed cryptosporidiosis patients and were uncommon (>7%) in control subjects. Faecal samples from human and animal sporadic cases yielded a wide range of cryptosporidial antigen banding patterns. Samples from patients in a water-borne outbreak in South Devon (England) in 1995 also yielded a wide range of banding patterns including members within individual household family groups. These results are in contrast with those from samples collected from other defined geographical areas, including some from a second water-borne outbreak where much more homogeneous banding patterns were obtained. Sera collected for other purposes from apparently uninfected individuals 9 months after the South Devon 1995 outbreak were examined. Antibodies to the three antigens of molecular weights 6, 14 and 17 kDa were detected in 32-49% of individuals resident in the outbreak water supply area, and in 15-21%) of those resident in an adjacent water supply area. The significance of these findings is discussed in relation to data obtained from epidemiological field studies.
The reactivity of a char depends very on the parent coal. Much information about correlation between properties of coal and reactivity of chars is lost by using only standard methods for a large dataset evaluation. In this research a set of 8 coals has been investigated by thermal analysis and reactivity of obtained chars was analyzed as a function of properties of parent coal properties using Canonical correlation analysis. The reactivity of chars was determined by thermogravimetric analysis of nonisothermal combustion in oxygen. It can be stated that methods of multivariate data analysis are useful tools for the interpretation of coal chars reactivity data., Mariusz Minkina, Elwira Zajusz-Zubek and Andrzej Mianowski., and Obsahuje bibliografii