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8782. Interview: "To je skutečně zcela nová fáze... čekáme nějakou novou fyziku"
- Creator:
- Kobayashi, Makoto, Smith, Adam, and Gregora, Ivan
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- Kobayashi, Makoto, 1944-, 20.-21. století, fyzici, Nobelova cena (ocenění), fyzika elementárních částic, physicists, Nobel Prizes, elementary particle physics, Japonsko, Japan, 6, and 53
- Language:
- Czech
- Description:
- Makoto Kobayashi, Adam Smith ; přeložil Ivan Gregora.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
8783. Intestinal microsporidiosis in African skink Mabuya perrotetii
- Creator:
- Koudela, Břetislav, Didier, Elisabeth S., Rodgers, Linda B., and Modrý, David
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- microsporidia, Reptilia, Encephalitozoon, electron microscopy, PCR-RFLP, and heteroduplex mobility
- Language:
- English
- Description:
- Intestinal microsporidiosis was documented by detecting abundant slightly curved spores (2.9 x 1.2 pm) in the faeces of five of twelve skinks Mabuya perrotetii Duméril et Bibron, 1839 that originated from Ghana. Clinically, the microsporidiosis was characterized by decreased appetite, diarrhea, and weight loss. Histopathological changes consisted of villous atrophy, blunting of mucosa and flattening of individual epithelial cells in the large intestine. The ultrastructure of microsporidian spores was consistent with an Encephalitozoon species. The PCR-RFLP assay and the heleroduplex mobility shift analyses were used to verify that the skink microsporidian is a species of the genus Encephalitozoon Levaditi, Nicolau et Schoen, 1923 and indicate that this microsporidian is not E. hellem, E. intestinalis or a strain of E. cuniculi. The microsporidia in African skink represent an Encephalitozoon species morphologically identical to Encephalitozoon lacerine Canning, 1981.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
8784. INTIMATIO PAEDAGOGII ACADEMICI, TRILINGVIS, TRIVII ...
- Creator:
- Mikuláš Albert z Kaménka and Daniel Adam z Veleslavína, dědicové (firma)
- Publisher:
- Adam z Veleslavína, Daniel - heredes
- Format:
- print and [6] ff ; 4°
- Type:
- model:monograph and TEXT
- Subject:
- století 17, pedagogika, intimace, and 094
- Language:
- Latin
- Description:
- PRAGÆ, Typis Hæredis M. Danielis Adami à Veleslavina 1612. and BCBT38341
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8785. Intoxikace a suicidia na interním oddělení – retrospektivní studie
- Creator:
- Krollová,, P., Novák, A., Ullrych, Martin, Kallmunzerová, D., Turčanová, B., Nguyen, H. T., Mikulica, M., Kadlečková, P., Pashagin, A., Sanaalah, A., and Holinková, E.
- Format:
- print, text, and regular print
- Type:
- model:article, article, Text, abstrakt z konference, and TEXT
- Language:
- Czech
- Description:
- P. Krollová, A. Novák, M. Ullrych, D. Kallmunzerová, B. Turčanová, H. T. Nguyen, M. Mikulica, P. Kadlečková, A. Pashagin, A. Sanaalah, E. Holinková
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8786. Intra-scleral parathalamus implant: efficacy and safety in rabbit eyes
- Creator:
- Detorakis, Efstathios T., Loggaki, Konstantina, Karyotakis, Nikolaos, and Ginis, Harilaos
- Format:
- print, text, and regular print
- Type:
- model:article, article, Text, časopisecké články, and TEXT
- Subject:
- zvířata, modely nemocí na zvířatech, ženské pohlaví, glaukom--chirurgie, nitrooční tlak, protézy a implantáty, králíci, and sklerostomie--metody
- Language:
- English
- Description:
- PURPOSE: A variety of anti-glaucomatous shunt designs have been proposed so far. This study evaluates the feasibility of a novel shunt design, the intra-scleral parathalamus implant (IPI), in a rabbit eye model. METHODS: Ten healthy albino rabbits were included. Measurements of the IOP, using TonoPenXL, as well as ocular rigidity (OR) and aqueous outflow facility (AO), using a previously described methodology, were performed in both eyes of each animal. Subsequently, the IPI was implanted at the left eye of all animals whereas the right eye served as control. Measurements of IOP were repeated at weekly intervals for 2 months postoperatively whereas measurements of OR and AO were repeated at the 1st and 2nd postoperative week. RESULTS: The IOP decreased significantly whereas the AO increased significantly postoperatively at the operated eyes. A significant postoperative decrease in OR was also recorded at the operated eyes. Respective differences at the fellow eyes were statistically not significant. Two animals developed postoperative complications and were excluded from follow-up. CONCLUSIONS: Results imply that the implantation of the IPI is feasible in rabbit eyes with significant favorable effects on the IOP, AO and OR. and E. T. Detorakis, K. Loggaki, N. Karyotakis, H. Ginis
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8787. Intracellular calcium in PTTH-stimulated prothoracic glands of Manduca sexta (Lepidoptera: Sphingidae)
- Creator:
- Birkenbeil, Heiner
- Format:
- print, text, and regular print
- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, fyziologie živočichů, hmyz, motýli, Sphingidae, Manduca sexta, prothorakální žlázy, ekdysteroidy, syntéza, stimulace, vápník, 595.2/.7, and 591.1
- Language:
- English
- Description:
- Larval Manduca prothoracic gland cells in vitro responded to prothoracicotropic hormone (PTTH) from neurosecretory cells of the brain with an increase of intracellular free calcium. This effect is reversible and dose-dependent. Preincubation of the glands with TMB-8 and dantrolene, which inhibit the release of calcium from intracellular stores, did not decrease the PTTH-stimulated increase in calcium, indicating that intracellular calcium stores are not involved in the control of ecdysteroidogenesis. Pharmacological studies of the PTTH effect with calcium channel blockers revealed that the increase in calcium was totally blocked by cadmium, partially inhibited by nickel and lanthanum and by amiloride, an antagonist of T-type calcium channels. All other inhibitors tested were ineffective, suggesting that the increase in cytosolic calcium is induced by opening of calcium channels, presumably of the T-type, in response to PTTH. The action of PTTH on these channels may be mediated by a G-protein as shown by the effect of mastoparan, a G-protein activator, which increased the concentration of cytosolic calcium comparable to that evoked by PTTH., Heiner Birkenbeil, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8788. Intracellular fate of polymer therapeutics investigated by fluorescence lifetime imaging and fluorescence pattern analysis
- Creator:
- Panek, J., Koziolova, E., Petr Štěpánek, Tomáš Etrych, and Olga Janoušková
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, polymery, polymers, therapeutics, anthracycline, confocal laser scanning microscopy, pattern matching, 14, and 612
- Language:
- English
- Description:
- Nanocarriers bearing anticancer drugs are promising candidates to improve the efficacy of cancer therapy and minimize side effects. The most potent cytostatics used in the treatment of various cancers are anthracyclines, e.g. doxorubicin or pirarubicin. Recently, polymer therapeutics carrying anthracyclines have been intensively studied. The precise characterization of in vitro nanocarrier biological behavior brings a better understanding of the nanocarrier characteristics and enables prediction of the behavior of the nanocarrier during in vivo application. Advanced fluorescence detection methods, e.g. fluorescence lifetime imaging microscopy (FLIM), were successfully exploited to describe the properties of various polymeric nano-systems and contributed to a complex view of anthracyclines’ intracellular transport and DNA intercalation. Here, we report the application of a specific technique for processing FLIM images, called fluorescence pattern decomposition, to evaluate early events after doxorubicin or pirarubicin treatment of cells. Moreover, we characterized changes in the intracellular localization and release of the anthracyclines during the incubation of cells with polymer nanotherapeutics based on poly[N-(2-hydroxypropyl)- methacrylamide] (pHPMA)., J. Panek, E. Koziolova, P. Stepanek, T. Etrych, O. Janouskova., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8789. Intracelullar mediators in regulation of leptin secretion from adipocytes
- Creator:
- Szkudelski, Tomasz
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, fyziologie, leptin, mastné kyseliny, adenosin, vápník, physiology, fatty acids, adenosine, calcium, adipocity, mTOR, adenosin trifostát (ATP), cyklický adenosinmonofosfát (cAMP), malonyl-CoA, adipocytes, mTOR (mammalian target of rapamycin), adenosine triphosphate (ATP), cyclic adenosine monophosphate (cAMP), 2, and 577
- Language:
- English
- Description:
- Leptin is a hormone primarily secreted by adipocytes and participating in the regulation of food intake and energy expenditure. Its blood levels usually correlate with adiposity. The secretion of this hormone is affected, among others, by food consumption, insulin, fasting and cold exposure. Regulation of leptin secretion depends on many intracellular events. It is known that the activation of mTOR (the mamma lian target of rapamycin) as well as increase in ATP and malonyl-CoA content in adipocytes enhance secretion of leptin. The rise in intracellular cAMP and fatty acids is thought to evoke the opposite effect. Moreover, the undisturbed action of endogenous adenosine in adipocytes and the proper intracellular Ca2+ concentration in these cells were also found to have an important function in leptin release. The role of mTOR, ATP, cAMP, fatty acids, malonyl-CoA, adenosine and Ca2+ in the regulation of leptin secretion from adipocytes is discussed., T. Szkudelski., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
8790. Intracerebroventricular injection of oxidant and antioxidant molecules affects long-term potentiation in urethane anaesthetized rats
- Creator:
- Viggiano, A., Viggiano, E., Monda, M., Ascione, S., Amaro, S., and de Luca, B.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Experimentální medicína, fyziologie, experimentální medicína, anestezie, physiology, experimental medicine, anesthesia, oxidant molecules, antioxidant molecules, intracerebroventricular injection, long-term potentiation, urethane anesthesia, 14, and 616-092
- Language:
- English
- Description:
- Production of superoxide anions in the incubation medium of hippocampal slices can induce long-term potentiation (LTP). Other reactive oxygen species (ROS) such as hydrogen peroxide are able to modulate LTP and are likely to be involved in aging mechanisms. The present study explored whether intracerebro-ventricular (ICV) injection of oxidant or antioxidant molecules could affect LTP in vivo. With this aim in mind, field excitatory post-synaptic potentials (fEPSPs) elicited by stimulation of the perforant pathway were recorded in the dentate gyrus of the hippocampal formation in urethane-anesthetized rats. N-acetyl-L-cysteine, hydrogen peroxide (H2O2) or hypoxanthine/xanthine-oxidase solution (a superoxide producing system) were administrated by ICV injection. The control was represented by a group injected with saline ICV. Ten minutes after the injection, LTP was induced in the granule cells of the dentate gyrus by high frequency stimulation of the perforant pathway. Neither the H2O2 injection or the N-acetyl-L-cysteine injection caused any variation in the fEPSP at the 10-min post-injection time point, whereas the superoxide generating system caused a significant increase in the fEPSP. Moreover, at 60 min after tetanic stimulation, all treatments attenuated LTP compared with the control group. These results show that ICV administration of oxidant or antioxidant molecules can modulate LTP in vivo in the dentate gyrus. Particularly, a superoxide producing system can induce potentiation of the synaptic response. Interestingly, ICV injection of oxidants or antioxidants prevented a full expression of LTP compared to the saline injection., A. Viggiano, E. Viggiano, M. Monda, A. Viggiano, S. Ascione, S. Amaro, B. de Luca., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public