zusammengestellt für den landwirtschaftlichen Bezirks-Verein in Leitmeritz von dem Ausschußmitgliede Herrn Anton Höchsmann, Název z obálky, and Tištěno švabachem
na základě nejlepších pramenů a vlastní zkušenosti k užitku českých hospodářů napsal Jan Datel, Obsahuje rejstřík, Přívazek k: Škůdcové řepy cukrovky z říše živočišné a jejich ničení / Antonín Kolárský CZ-PrNK, and Converted from MODS 3.5 to DC version 1.8 (EE patch 2018/01/18)
AIMS: Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties. METHODS: DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. RESULTS: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 ± 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 ± 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 ± 10.4 hours), 2% PRP (DT 40.1 ± 5.7 hours) and 2% HP (DT 49.0 ± 15.2 hours) showed almost the same proliferative activity. DPSC's viability in the 9th passage was over 90% except for the DPSC cultivated in the 10% HP media. CONCLUSIONS: We proved that human blood components are suitable substitution for FCS in cultivation media for long-term DPSC cultivation. and T. Suchánková Kleplová, T. Soukup, V. Řeháček, J. Suchánek
Dr. techn. PhDr. Jaroslav Just a Ing. Vojtěch Straka, Nad názvem: Zemědělská rada pro království České. Český odbor, Číslo tisku CCLII. Číslo jednací 21512/1917, and Converted from MODS to DC version 1.8 (EE patch 2018/05/24)