The European stag beetle, Lucanus cervus, is recognised as a flagship species for biodiversity conservation. Although the species is widely distributed across Europe declines have led to it being granted protected or endangered status in a number of countries and regarded as "near threatened" by the IUCN. The integration of genetic approaches into conservation efforts is urgently needed but has been impeded to date by the lack of appropriate genetic markers. To provide such a resource the development of the first microsatellite loci for stag beetle is described. Loci were identified using two methods (i) enriched library cloning (ELC) and (ii) Restriction enzyme Associated DNA sequencing (RAD-Seq). Inefficient microsatellite detection using the ELC method suggests that RAD-Seq, or other Next Generation Sequencing based methods, may ultimately be more cost effective for obtaining informative suites of markers for this and other coleopteran species. 18 loci were characterised by genotyping 42 UK specimens collected as prey leftover/roadkill. All loci produced unambiguous genotypes and were polymorphic. Though preliminary, estimates of genetic variability suggest UK populations may be genetically depauperate. The microsatellite loci represent a suite of genetic markers that can be applied to non-invasive population monitoring and numerous other areas of Lucanus conservation and evolutionary research., Niall J. McKeown, Deborah J. Harvey, Amy J. E. Healey, Ilze Skujina, Karen Cox, Alan C. Gange, Paul W. Shaw., and Obsahuje bibliografii
In this study, we report the development of a set of 15 polymorphic microsatellite markers for the box tree moth, Cydalima perspectalis (Walker), a highly invasive insect in Europe causing significant damage to natural and ornamental Buxus trees. The markers were characterized for four distant populations in both its native (China, two populations) and invasive ranges (Czech Republic and Turkey, one population each). The number of alleles ranged from 2 to 12. No marker significantly deviated from the Hardy-Weinberg equilibrium for all the populations sampled. These microsatellite markers are promising tools for further studies on the invasive pathways and dispersal pattern of the box tree moth in Europe., Audrey Bras, Laure Sauné, Alain Roques, Jérôme Rousselet, Marie-Anne Auger-Rozenberg., and Obsahuje bibliografii
This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. Eight of the nine loci described were found to be polymorphic. The number of alleles ranged from three to 11. Observed and expected heterozygosity on the population level ranged from 0.135 to 0.891 and 0.177 to 0.865, respectively. Genotypic analyses revealed no deviations from Hardy-Weinberg equilibrium or linkage disequilibrium after sequential Bonferroni correction. Cross-species amplification was also tested for nine related species and positive amplifications were found in most of the assays. These markers will be useful in studies involving relatedness analysis and investigations of the population genetics of these wasps., Juliano Da Costa Almeida ... [et al.]., and Obsahuje seznam literatury
Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle., Oddmund Kleven, Anders Endrestøl., and Obsahuje bibliografii
Sexual selection is thought to result in an elongation of genitalia in insects. Part of the genitalia of the leaf beetle Lema (Lema) coronata Baly is approximately twice the body length in both sexes. Nine microsatellite loci were isolated from L. (L.) coronata for use in future studies on the correlation between genital length and reproductive success. The nine loci were polymorphic, with three to six alleles per locus recorded in 40 individuals. Observed heterozygosity ranged from 0.08 to 0.38 and expected heterozygosity from 0.51 to 0.75. The non-exclusive probability of the second parent and the identity calculated from the set of loci were 0.011 and < 0.001, respectively. A deviation from the Hardy-Weinberg equilibrium was detected at all nine loci, i.e. significant heterozygote deficiencies, and several hypotheses accounting for this are discussed. The isolated loci carried independent information. Five polymorphic loci were amplified in a related sympatric species, L. (L.) scutellaris, which has relatively short genitalia. These microsatellite makers will be used to determine the paternity of offspring of females multiply mated in the laboratory and compare the selection pressures operating on genital length in these closely related species. and Yoko Matsumura, Izumi Yao.
Genetically modified (GM) maize may be - come important as the spread of the Western Corn Rootworm (Diabrotica virgifera) feeding on maize continues. However, its implementation in european agriculture has been hindered by fears of undesirable environmental effects, despite the worldwide cultivation of the GM crop. The presented study compares impacts of the GM maize MON 88017 and the non-transgenic maize on non-target invertebrates. Neither the composition nor the species abundance of invertebrate communities were affected in a field with the GM maize. and Zdeňka Svobodová, Oxana Habuštová, František Sehnal.
Společenstva mravenců většinou reagují na změny jejich biotopů méně výrazně než společenstva jiných organismů. V biotopech měnících se pozvolněji (např. neobhospodařované louky) se zpravidla dlouho zachovává původní druhová skladba myrmekofauny, zatímco výměna druhů z jiných skupin (rostlin i většiny bezobratlých) je mnohem rychlejší. Podobně na člověkem výrazně pozměněných lokalitách typu výsypek, odkališť nebo pískoven společenstvo mravenců velmi často sestává pouze z několika běžných druhů, zatímco mezi ostatními organismy se obyčejně najde řada druhů z Červené knihy. Mezi příčiny těchto rozdílů patří zejména značná schopnost mravenců regulovat podmínky panující v hnízdě, jejich agresivita a způsob šíření. Nově vzniklou lokalitu nejrychleji osídlí druhy mravenců běžné v okolí, kdežto obecně vzácnější specialisté mají zpoždění a již existující kolonie dříve příchozích je na lokalitu nepustí. Na druhou stranu z výskytu kolonií druhů na místech pro ně nevhodných může ukazovat, že na těchto lokalitách panovaly v uplynulých letech podmínky odlišné., Ant assemblages are usually less affected by environmental alteration than other organisms. They persist without obvious changes in slowly changing habitats (e.g. unmanaged meadows) much longer than assemblages of plants or most invertebrates. Similarly, communities of ants consist of several common species in anthropogenic habitats (spoil dumps, coal ash settling basins or sandpits) whereas assemblages of other organisms usually contain some rare and endangered species. The ability of ants to regulate environmental conditions in nests, their agressivity and mode of dispersion play an important role in this respect. A new locality is often first colonised by common ant species. The occurrence of colonies of certain species in habitats with suboptimal conditions can indicate the past changes of local environmental conditions., and Pavel Pech.
Ips typographus is an important pest of Eurasian spruce monoculture. Recent outbreaks in the Šumava National Park started extensive political and public discussions related mainly to the questions of pest management in natural protected areas. The article reviews the biology and some aspects of the physiology and ecophysiology of this species. and Petr Doležal.