Two species of the genus Gryllus occur in Europe: G. campestris and G. bimaculatus. The first is widely distributed in the north-western Palaearctic, while the second, G. bimaculatus, occurs predominantly in the Mediterranean area. There is a visible pattern in the distribution of G. campestris, the insect being rare and threatened in the western part of its range, whereas it is still abundant in the east. Despite the fact that this species is commonly used in laboratory experiments, its natural populations are poorly characterised. In the present study, we analysed cricket populations from the lower Oder and Vistula River valleys in Poland. Based on the phylogeny of the mtDNA cytochrome b fragment, we found that 17% of the individuals studied had a G. bimaculatus-like mtDNA haplotype. Analyses of 11 autosomal microsatellite loci failed to reveal any clear genetic differentiation between individuals assigned to these two clades. This suggests, along with the spatial distribution of G. bimaculatus-like haplotypes, successful interbreeding of G. bimaculatus with native populations of G. campestris. However, both the nuclear data and additional analyses of two X-chromosome-linked microsatellite loci revealed incomplete introgression. Human-mediated introgression seems to be the most plausible explanation of the observed genotypic pattern such that caution needs to be taken in conservation efforts carried out in the western part of the species' range., Hanna Panagiotopoulou, Mateusz Baca, Katarzyna Baca, Paweł Sienkiewicz, Piotr Ślipiński, Michał Żmihorski., and Obsahuje bibliografii
Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle., Oddmund Kleven, Anders Endrestøl., and Obsahuje bibliografii
Twelve polymorphic microsatellite loci were developed and characterized from the bamboo locust, Rammeacris kiangsu, based on enriched genomic libraries. Analysis of 30 individuals showed that the number of alleles ranged from seven to 25 with the observed heterozygosity ranging from 0.333 to 0.767 and expected heterozygosity from 0.784 to 0.963. Test of cross-species amplification showed that some of these microsatellite markers could be used for studying other species such as Ceracris nigricornis, C. fasciata, and Chorthippus brunneus.