Béthoux recently identified the species †Adiphlebia lacoana Scudder from the Carboniferous of Mazon Creek, Ill., USA as the oldest beetle. The fossils bear coriaceous tegmina with pseudo-veins allegedly aligned with "rows of cells" as they occur in Permian beetles and extant Archostemata. The examination of four new specimens of †Adiphlebia lacoana from the same locality revealed that the "cells" are in fact clumps of clay inside a delicate meshwork, and no derived features shared with Coleoptera or Coleopterida (= Coleoptera + Strepsiptera) were found. Instead, †Adiphlebia lacoana bears veinal fusions and braces similar to extant Neuroptera. These features support a placement in †Strephocladidae, and are also similar to conditions found in †Tococladidae. These unplaced basal holometabolan families were erroneously re-analyzed as ancestral Mantodea and Orthoptera. Homologization of the wing pairs in neopteran lineages is updated and identification errors are corrected. A new Permian beetle family †Moravocoleidae [†Protocoleoptera (= Permian Coleoptera with pointed unpaired ovipositor; e.g., †Tshekardocoleidae)] is described., Jarmila Kukalová-Peck, Rolf G. Beutel., and Obsahuje seznam literatury
This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. Eight of the nine loci described were found to be polymorphic. The number of alleles ranged from three to 11. Observed and expected heterozygosity on the population level ranged from 0.135 to 0.891 and 0.177 to 0.865, respectively. Genotypic analyses revealed no deviations from Hardy-Weinberg equilibrium or linkage disequilibrium after sequential Bonferroni correction. Cross-species amplification was also tested for nine related species and positive amplifications were found in most of the assays. These markers will be useful in studies involving relatedness analysis and investigations of the population genetics of these wasps., Juliano Da Costa Almeida ... [et al.]., and Obsahuje seznam literatury
Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle., Oddmund Kleven, Anders Endrestøl., and Obsahuje bibliografii
Sexual selection is thought to result in an elongation of genitalia in insects. Part of the genitalia of the leaf beetle Lema (Lema) coronata Baly is approximately twice the body length in both sexes. Nine microsatellite loci were isolated from L. (L.) coronata for use in future studies on the correlation between genital length and reproductive success. The nine loci were polymorphic, with three to six alleles per locus recorded in 40 individuals. Observed heterozygosity ranged from 0.08 to 0.38 and expected heterozygosity from 0.51 to 0.75. The non-exclusive probability of the second parent and the identity calculated from the set of loci were 0.011 and < 0.001, respectively. A deviation from the Hardy-Weinberg equilibrium was detected at all nine loci, i.e. significant heterozygote deficiencies, and several hypotheses accounting for this are discussed. The isolated loci carried independent information. Five polymorphic loci were amplified in a related sympatric species, L. (L.) scutellaris, which has relatively short genitalia. These microsatellite makers will be used to determine the paternity of offspring of females multiply mated in the laboratory and compare the selection pressures operating on genital length in these closely related species. and Yoko Matsumura, Izumi Yao.
Genetically modified (GM) maize may be - come important as the spread of the Western Corn Rootworm (Diabrotica virgifera) feeding on maize continues. However, its implementation in european agriculture has been hindered by fears of undesirable environmental effects, despite the worldwide cultivation of the GM crop. The presented study compares impacts of the GM maize MON 88017 and the non-transgenic maize on non-target invertebrates. Neither the composition nor the species abundance of invertebrate communities were affected in a field with the GM maize. and Zdeňka Svobodová, Oxana Habuštová, František Sehnal.
Společenstva mravenců většinou reagují na změny jejich biotopů méně výrazně než společenstva jiných organismů. V biotopech měnících se pozvolněji (např. neobhospodařované louky) se zpravidla dlouho zachovává původní druhová skladba myrmekofauny, zatímco výměna druhů z jiných skupin (rostlin i většiny bezobratlých) je mnohem rychlejší. Podobně na člověkem výrazně pozměněných lokalitách typu výsypek, odkališť nebo pískoven společenstvo mravenců velmi často sestává pouze z několika běžných druhů, zatímco mezi ostatními organismy se obyčejně najde řada druhů z Červené knihy. Mezi příčiny těchto rozdílů patří zejména značná schopnost mravenců regulovat podmínky panující v hnízdě, jejich agresivita a způsob šíření. Nově vzniklou lokalitu nejrychleji osídlí druhy mravenců běžné v okolí, kdežto obecně vzácnější specialisté mají zpoždění a již existující kolonie dříve příchozích je na lokalitu nepustí. Na druhou stranu z výskytu kolonií druhů na místech pro ně nevhodných může ukazovat, že na těchto lokalitách panovaly v uplynulých letech podmínky odlišné., Ant assemblages are usually less affected by environmental alteration than other organisms. They persist without obvious changes in slowly changing habitats (e.g. unmanaged meadows) much longer than assemblages of plants or most invertebrates. Similarly, communities of ants consist of several common species in anthropogenic habitats (spoil dumps, coal ash settling basins or sandpits) whereas assemblages of other organisms usually contain some rare and endangered species. The ability of ants to regulate environmental conditions in nests, their agressivity and mode of dispersion play an important role in this respect. A new locality is often first colonised by common ant species. The occurrence of colonies of certain species in habitats with suboptimal conditions can indicate the past changes of local environmental conditions., and Pavel Pech.
Ips typographus is an important pest of Eurasian spruce monoculture. Recent outbreaks in the Šumava National Park started extensive political and public discussions related mainly to the questions of pest management in natural protected areas. The article reviews the biology and some aspects of the physiology and ecophysiology of this species. and Petr Doležal.