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32. Insect cold tolerance: How many kinds of frozen?
- Creator:
- Sinclair, Brent J.
- Format:
- print, text, and regular print
- Type:
- article, bibliography, conference publication, model:article, and TEXT
- Subject:
- Third European Workshop of Invertebrate Ecophysiology (1998 : Birmingham, Česko), zoologie, fyziologie živočichů, ekofyziologie, hmyz, tolerance k chladu, tolerance k mrazu, netolerance k mrazu, teplotní strategie, 591.5, 061.3, and 595.2/.7
- Language:
- English
- Description:
- Insect cold tolerance mechanisms are often divided into freezing tolerance and freeze intolerance. This division has been criticised in recent years; Bale (1996) established five categories of cold tolerance. In Bale's view, freezing tolerance is at the extreme end of the spectrum of cold tolerance, and represents insects which are most able to survive low temperatures. Data in the literature from 53 species of freezing tolerant insects suggest that the freezing tolerance strategies of these species are divisible into four groups according to supercooling point (SCP) and lower lethal temperature (LLT): (1) Partially Freezing Tolerant-species that survive a small proportion of their body water converted into ice, (2) Moderately Freezing Tolerant-species die less than ten degrees below their SCP, (3) Strongly Freezing Tolerant-insects with LLTs 20 degrees or more below their SCP, and (4) Freezing Tolerant Species with Low Supercooling Points which freeze at very low temperatures, and can survive a few degrees below their SCP. The last 3 groups can survive the conversion of body water into ice to an equilibrium at sub-lethal environmental temperatures. Statistical analyses of these groups are presented in this paper. However, the data set is small and biased, and there are many other aspects of freezing tolerance, for example proportion of body water frozen, and site of ice nucleation, so these categories may have to be revised in the future. It is concluded that freezing tolerance is not part of Bale's (1996) continuum, but rather a parallel, alternative strategy of cold tolerance., Brent J. Sinclair, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
33. Insemination and fertilization in the seed bug Lygaeus simulans (Heteroptera: Lygaeidae)
- Creator:
- Micholitsch, Thomas, Krügel, Peter, and Pass, Günther
- Format:
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- Type:
- article, model:article, and TEXT
- Subject:
- Lygaeidae, kopulace, entomologie, hmyz, ploštice, Lygaeus simulans, kopulační orgány, morfologie, inseminace, fertilizace, Heteroptera, genitalia, copulation, cryptic female choice, sperm utilization, spermatheca, aedeagus, processus gonophori, insemination, fertilization, 595.2/.7, and 591.4
- Language:
- English
- Description:
- In the laboratory, not all females of the seed bug Lygaeus simulans Deckert, 1985, produced fertilized eggs after copulation: 26.7% of the females were not inseminated and 5% were inseminated but did not lay fertilized eggs; only in 40% of the couples did copulation result in fertile eggs. The remaining 28.3% of couples refrained from mating. Duration of copulation was associated with insemination and fertilization: (i) fertile eggs were produced by only one couple that copulated for less than 60 min and all those that copulated for more than 360 min, (ii) probability of fertilization increased steadily with duration of copulation between 60 and 360 min, and (iii) duration of copulation was significantly different for couples that showed different insemination status. A possible morphological explanation for this rime dependency was revealed by examining the genitalia of 69 couples freeze-fixed in copula after different periods in copulation. Because of the intricate structure of the genitalia in L. simulans, a male takes a long time to manoeuver its intromittent organ into the narrow insemination duct of the female. Only if completely inserted is the tip of the intromittent organ close enough for successful ejaculation of sperm into the spermatheca. The freeze-fixing experiment revealed that it usually took the male more than 30 min to locate the entrance to the insemination duct and another 30 min for full penetration. This explains why copulations that lasted less than 60 min failed, since insemination began only after intromission was complete. The experiments, therefore, indicated that there is a relationship between the complex morphology of the genitalia and the low rates of insemination and fertilization in L. simulans., Thomas Micholitsch, Peter Krügel, Günther Pass, 7 obrázků, 1 tab., and Lit.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
34. Intracellular calcium in PTTH-stimulated prothoracic glands of Manduca sexta (Lepidoptera: Sphingidae)
- Creator:
- Birkenbeil, Heiner
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- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, fyziologie živočichů, hmyz, motýli, Sphingidae, Manduca sexta, prothorakální žlázy, ekdysteroidy, syntéza, stimulace, vápník, 595.2/.7, and 591.1
- Language:
- English
- Description:
- Larval Manduca prothoracic gland cells in vitro responded to prothoracicotropic hormone (PTTH) from neurosecretory cells of the brain with an increase of intracellular free calcium. This effect is reversible and dose-dependent. Preincubation of the glands with TMB-8 and dantrolene, which inhibit the release of calcium from intracellular stores, did not decrease the PTTH-stimulated increase in calcium, indicating that intracellular calcium stores are not involved in the control of ecdysteroidogenesis. Pharmacological studies of the PTTH effect with calcium channel blockers revealed that the increase in calcium was totally blocked by cadmium, partially inhibited by nickel and lanthanum and by amiloride, an antagonist of T-type calcium channels. All other inhibitors tested were ineffective, suggesting that the increase in cytosolic calcium is induced by opening of calcium channels, presumably of the T-type, in response to PTTH. The action of PTTH on these channels may be mediated by a G-protein as shown by the effect of mastoparan, a G-protein activator, which increased the concentration of cytosolic calcium comparable to that evoked by PTTH., Heiner Birkenbeil, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
35. Juvenile hormone synthesis by corpora allata of tomato moth Lacanobia oleracea (Lepidoptera: Noctuidae), end the effects of allatostatins and allatotropin in vitro
- Creator:
- Audsley, Neil, Weaver, Robert J., and Edwards, John P.
- Format:
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- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, fyziologie živočichů, hmyz, motýli, Noctuidae, Lacanobia oleracea, corpora allata, juvenilní hormony, biosyntéza, kontrola biosyntézy, neuropeptidy, allatostatiny, allatotropiny, účinky, pokusy in vitro, 595.2/.7, and 591.1
- Language:
- English
- Description:
- The nature and rate of juvenile hormone (JH) biosynthesis and effects of allatostatins and allatotropin have been investigated in isolated corpora allata (CA) of adults and larvae of the noctuid tomato moth, Lacanobia oleracea. In adult female CA, mean rates of synthesis were relatively constant (10-16 pmol/pr/h) at all times. However, the range of JH synthesis by individual CA of similarly aged insects was quite large (2-30 pmol/pr/h). High performance liquid chromatography (HPLC) separation shows that adult female moth CA synthesise predominantly JH I and JH II. Rates of JH synthesis in vitro are dependent on methionine concentration. Synthetic Manduca sexta allatostatin (Mas-AS) caused a dose-dependent inhibition of JH synthesis by adult female CA but only to a max. of 54%, whilst 10 µM synthetic M. sexta allatotropin caused a 37% stimulation of CA activity. At 1 mM the cockroach allatostatin, Dip-allatostatin-2, had no significant effect on JH synthesis. In larval L. oleracea, rates of JH biosynthesis were very low., Neil Audsley, Robert J. Weaver, John P. Edwards, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
36. Life cycles in polar arthropods - flexible or programmed?
- Creator:
- Danks, Hugh V.
- Format:
- print, text, and regular print
- Type:
- article, bibliography, conference publication, model:article, and TEXT
- Subject:
- Arctic, Third European Workshop of Invertebrate Ecophysiology (1998 : Birmingham, Česko), climate, temperature, seasonal, life-cycle, arthropods, insects, polar, Antarctic, zoologie, fyziologie živočichů, ekofyziologie, členovci, hmyz, životní cykly, sezónní vlivy, klimatické vlivy, teplota, arktická oblast, antarktická oblast, fenologická studie, přehledy, 591.5, 061.3, and 595.2/.7
- Language:
- English
- Description:
- Climate features that influence life cycles, notably severity, seasonality, unpredictability and variability, are summarized for different polar zones. The zones differ widely in these factors and how they are combined. For example, seasonality is markedly reduced by oceanic influences in the Subantarctic. Information about the life cycles of Arctic and Antarctic arthropods is reviewed to assess the relative contributions of flexibility and programming to life cycles in polar regions. A wide range of life cycles occurs in polar arthropods and, when whole life cycles are considered, fixed or programmed elements are well represented, in contrast to some recent opinions that emphasize the prevalence of flexible or opportunistic responses. Programmed responses ale especially common for controlling the appearance of stages that are sensitive to adverse conditions, such as the reproductive adult. The relative contribution of flexibility and programming to different life cycles is correlated with taxonomic affinity (which establishes the general lifecycle framework for a species), and with climatic zone, the habitats of immature and adult stages, and food., Hugh V. Danks, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
37. Metabolic neurohormones: release, signal transduction and physiological responses of adipokinetic hormones in insects
- Creator:
- Van Der Horst, Dick J., Van Marrewijk, Wil J. A., Vullings, Henk G. B., and Diederen, Jacques H. B.
- Format:
- print, text, and regular print
- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, entomologie, hmyz, Locusta migratoria, metabolismus, metabolické neurohormony, adipokinetické hormony, biosyntéza, vylučování, přenos signálu, mechanismus přenosu, 595.2/.7, and 591.1
- Language:
- English
- Description:
- In view of the extremely high metabolic rates involved, insect flight offers a fascinating model system for studying metabolism during exercise, including its regulation by metabolic neurohormones. In our laboratory the African migratory locust, Locusta migratoria, well-known for its long-distance flights, is used as an internationally recognized model insect. The insect is mass-reared under controlled conditions; its size permits convenient handling in vivo and in vitro, while flight activity can be easily evoked. In addition, research on this pest insect may be of economical importance. A survey of the energy metabolism during locust flight is presented in Fig. 1. Flight activity stimulates the neurosecretory adipokinetic cells in the glandular lobes of the corpus cardiacum, a neuroendocrine gland connected with the insect brain, to release peptide neurohormones, the adipokinetic hormones (AKHs). The target for these hormones is the fat body. Via signal transduction processes, the action of the hormones ultimately results in the mobilization of both carbohydrate and lipid reserves as fuels for flight. Carbohydrate (trehalose) is mobilized from glycogen reserves, implying hormonal activation of the key enzyme, fat body glycogen phosphorylase, by phosphorylation. Similarly, on the lipid side, sn-1,2-diacylglycerol (DAG) is mobilized from stored triacylglycerol (TAG), by hormonal activation of the fat body TAG lipase. The carbohydrate and lipid substrates are transported in the hemolymph to the contracting flight muscles. Carbohydrate provides most of the energy for the initial period of flight, whereas at a later stage, lipid substrate in the blood is increased and gradually takes over. The transport of DAG requires specific lipoprotein carriers (lipophorins) which differ in several respects from the lipoproteins in mammals, and act as a lipid shuttle. This review is focused on three interrelated topics, covering recent data on the biosynthesis and release of the AKHs, their signal transduction mechanisms in the fat body cells, and the changes in the lipophorin system induced by the AKHs during flight., Dick J. Van Der Horst, Wil J.A. Van Marrewijk, Henk G.B. Vullings, Jacques H.B. Diederen, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
38. Monophyly of the subgenus Leptempis, and description of seven new species of the Empis (Leptempis) rustica-group (Diptera: Empididae)
- Creator:
- Daugeron, , Christophe
- Format:
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- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, entomologie, hmyz, dvoukřídlí, Empididae, Empis, Leptempis, Leptempis rustica, nové druhy, taxonomie, fylogeneze, monofylie, and 595.2/.7
- Language:
- English
- Description:
- The monophyly of the subgenus Leptempis Collin of the genus Empis L. is established on the basis of a male hypopygial character, and the possibility of a close relationship between the subgenera Leptempis Collin, Planempis Frey and Kritempis Collin is discussed. Seven new species belonging to Empis (Leptempis) rustica-group are described from France, Germany, Greece and Spain: E. (L.) abdominalis sp. n., E. (L.) lamellata sp. n., E. (L.) multispina sp. n., E. (L.) pandellei sp. n., E. (L.) lamellimmanis sp. n., E. (L.) sinuosa sp. n. and E. (L.) trunca sp. n. A key to the E. (L.) rustica-group is presented., Christophe Daugeron, and Lit
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- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
39. Myotropic neuropeptides from the retrocerebral complex of the stick insect, Carausius morosus (Phasmatodea: Lonchodidae)
- Creator:
- Predel, Reinhard, Kellner, Roland, and Gäde, Gerd
- Format:
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- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, hmyz, Phasmatodea, Lonchodidae, Carausius morosus, neuropeptidy, myotropin, myotropické neuropeptidy, izolace, bioaktivita, 595.2/.7, and 591.1
- Language:
- English
- Description:
- Myotropic neuropeptides were isolated from the retrocerebral complex of the stick insect, Carausius morosus, by using three HPLC steps. Bioactivity during purification was measured by heterologous bioassays monitoring the contractions of the hyperneural muscle and hindgut of the American cockroach. Additionally, fractions not active in these bioassays were tested in a homologous bioassay evoking contractions of the hindgut of C. morosus. Peptide sequence analysis and mass spectrometry yielded the following structures: Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2 (CCAP), pGlu-Thr-Phe-Gln-Tyr-Ser-His-Gly-Trp-Thr-Asn-NH2 (His7-corazonin) and Asp-Glu-Gly-Gly-Thr-Gln-Tyr-Thr-Pro-Arg-Leu-NH2 (Cam-PK-1). These neuropeptides are the first myotropins isolated from C. morosus. The most bioactive compound in the homologous bioassay, the C. morosus-hindgut assay, was CCAP., Reinhard Predel, Roland Kellner, Gerd Gäde, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
40. Periods of dormancy and cohort-splitting in the millipede Polydesmus angustus (Diplopoda: Polydesmidae)
- Creator:
- David, Jean-François, Celerier, Marie-Louise, and Geoffroy, Jean-Jacques
- Format:
- print, text, and regular print
- Type:
- article, bibliography, conference publication, model:article, and TEXT
- Subject:
- Third European Workshop of Invertebrate Ecophysiology (1998 : Birmingham, Česko), zoologie, fyziologie živočichů, ekofyziologie, hmyz, Diplopoda, Polydesmus angustus, životní cyklus, reprodukční dormance, diapauza, sezónní vlivy, 591.5, 061.3, and 595.2/.7
- Language:
- English
- Description:
- First stadium juveniles of P. angustus were reared under controlled seasonal conditions to maturity, reproduction and death. Individuals born in any one breeding season either had a 1-year or a 2-year life cycle (cohort-splitting). The life cycle was annual for individuals born in the first part of the breeding season (May-August), but became biennial for those born later (August-October). Two phenomena were involved: (1) Only individuals reaching the penultimate stadium (stadium VII) before a critical period at the end of spring could become adult in the breeding season following that of their birth. After this time, stadium VII individuals entered into aestivation and only became adult in the second autumn after their birth. (2) Females becoming adult in autumn entered reproductive dormancy and only laid eggs in the following spring. Overall, individuals born at the start of the breeding season easily reached stadium VII before the critical period and were able to breed at I year, whereas individuals born at the end of the breeding season reached stadium VII after the critical period, then had two consecutive periods of dormancy and only bred at 2 years age. Individuals from the same nest born in the middle of the breeding season (August) could have either annual or biennial life cycles, depending on whether they reached stadium VII before or during aestivation. The environmental factors capable of triggering aestivation in subadults and reproductive dormancy in autumn-maturing females are discussed., Jean-Francois David, Marie-Louise Celerier, Jean-Jacques Geoffroy, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public