Number of results to display per page
Search Results
30782. Přesnost a pravdivost výsledků měření - hledání souvislostí
- Creator:
- Friedecký, Bedřich
- Type:
- model:article, article, Text, úvodníky, and TEXT
- Subject:
- referenční hodnoty, referenční standardy, prediktivní hodnota testů, and biologické markery--analýza
- Language:
- Czech and English
- Description:
- Bedřich Friedecký and Lit.: 7
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
30783. Pressing Down Lemma for $\lambda $-trees and its applications
- Creator:
- Li, Hui and Peng, Liang-Xue
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- tree, $D$-space, $\lambda $-tree, property $\gamma $, and collectionwise Hausdorff
- Language:
- English
- Description:
- For any ordinal $\lambda $ of uncountable cofinality, a $\lambda $-tree is a tree $T$ of height $\lambda $ such that $|T_{\alpha }|<{\rm cf}(\lambda )$ for each $\alpha <\lambda $, where $T_{\alpha }=\{x\in T\colon {\rm ht}(x)=\alpha \}$. In this note we get a Pressing Down Lemma for $\lambda $-trees and discuss some of its applications. We show that if $\eta $ is an uncountable ordinal and $T$ is a Hausdorff tree of height $\eta $ such that $|T_{\alpha }|\leq \omega $ for each $\alpha <\eta $, then the tree $T$ is collectionwise Hausdorff if and only if for each antichain $C\subset T$ and for each limit ordinal $\alpha \leq \eta $ with ${\rm cf}(\alpha )>\omega $, $\{{\rm ht}(c)\colon c\in C\} \cap \alpha $ is not stationary in $\alpha $. In the last part of this note, we investigate some properties of $\kappa $-trees, $\kappa $-Suslin trees and almost $\kappa $-Suslin trees, where $\kappa $ is an uncountable regular cardinal.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
30784. Pressure overload selectively increases n-3 PUFA in myocardial phospholipids during early postnatal period
- Creator:
- Novák, F., František Kolář, Voců, Š., Marek Vecka, and Olga Nováková
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, phospholipids, n-3 PUFA, myocardium, postnatal development, pressure overload, rat, 14, and 612
- Language:
- English
- Description:
- Increasing hemodynamic load during early postnatal development leads to rapid growth of the left ventricular (LV) myocardium, which is associated with membrane phospholipid (PL) remodeling characterized by n-3 polyunsaturated fatty acids (PUFA) accumulation. The aim of this study was to examine the influence of additional workload imposed early after birth when ventricular myocytes are still able to proliferate. Male Wistar rats were subjected to abdominal aortic constriction (AC) at postnatal day 2. Concentrations of PL and their fatty acid (FA) profiles in the LV were analyzed in AC, sham-operated (SO) and intact animals on postnatal days 2 (intact only), 5 and 10. AC resulted in LV enlargement by 22 % and 67 % at days 5 and 10, respectively, compared with age-matched SO littermates. Concentrations of phosphatidylcholine, cardiolipin, phosphatidylinositol, phosphatidylethanolamine, phosphatidylserine and sphingomyelin decreased in AC myocardium, albeit with different time course and extent. The main effect of AC on FA remodeling consisted in the accumulation of n-3 PUFA in PL. The most striking effect of AC on FA composition was observed in phosphatidylinositol and cardiolipin. We conclude that excess workload imposed by AC inhibited the normal postnatal increase of PL concentration while further potentiating the accumulation of n-3 PUFA as an adaptive response of the developing myocardium to accelerated growth., F. Novák, ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
30785. Pressure wave cardiography; cardiac diagnosis by means of noninvasive continuous blood pressure record
- Creator:
- Fišer, B., Honzíková, N., and Semrád, B.
- Type:
- article, model:article, and TEXT
- Subject:
- blood pressure monitoring, interval-strength relationship, congestive heart failure, and atrial fibrillations
- Language:
- English
- Description:
- The pulse pressure (PP) is proportional to the preceding interval (T) because of the restitution of contractility and the Starling mechanism, and is inversely proportional to the pre-preceding interval (T-l) because of potentiation of contractility. The aim of the present paper was to find if this relationship can be used for diagnostic purposes. Blood pressure was noninvasively and continuously recorded for 3 minutes (Pen^z method), in 26 healthy subjects, in 13 patients with congestive heart failure (NYHA I,II) and sinusoidal rhythms and in 21 patients with atrial fibrillations. By means of multidimensional regression analysis the coefficient D[T] and D[T-1] were calculated in each subject. D[T] expresses the relative role of the preceding, D[T-1] of the pre-preceding interval. The correlation between PP and T was small in subjects with sinusoidal rhythms. Subjects with particular correlation coefficients between PP and T-l higher than 0.5 were used for further analysis (18 controls, 7 patients). The difference between D[T-1] in controls (0.30 ± 0.20) and in patients (0.48 ± 0.19) was significant (Wilcoxon P<0.05). In subjects with atrial fibrillations both D[T] and D[T-1] were higher in decompensated patients (Wilcoxon P<0.05). The ratio D[T]/D[T-1] was higher in patients with mitral stenosis than in patients with ischaemic heart disease (t-test, P<0.05). The test can be usefully employed as a screening test in medical practice.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
30786. Preston C. D., Pearman D. A. & Dines T. D. (eds.). New atlas of the British and Irish Flora. An atlas of the vascular plants of Britain, Ireland, the Isle of Man and the Channel Islands
- Creator:
- Petřík, Petr
- Type:
- article and TEXT
- Language:
- English
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/
30787. Presumptivní kombinované HPLC stanovení fetálního hemoglobinu využitím modu pro glykovaný hemoglobin
- Creator:
- Kukačka, Jiří, Klapková, E., and Průša, Richard
- Type:
- model:article, article, Text, and TEXT
- Subject:
- chromatografie vysokotlaká kapalinová--přístrojové vybavení, fetální hemoglobin--analýza, and hemoglobin A glykosylovaný--analýza
- Language:
- Czech and English
- Description:
- Cíl: Stanovení fetálního hemoglobinu (HbF) je důležité pro diagnostiku hemoglobinopatií a jiných onemocnění. Cílem naší práce bylo zhodnotit možnost presumptivního stanovení HbF v modu pro stanovení glykovaného hemoglobinu (HbA1c) na automatickém HPLC analyzátoru Tosoh HLC-723 G7. Materiál a metody: K analýze HbF v modu pro stanovení HbA1c bylo použito 63 vzorků nesrážlivé krve. Vzorky byly rozděleny do 3 skupin: 1. skupina (n = 24), HbF v intervalu 1–2 % z celkového hemoglobinu (Hb); 2. skupina (n = 21), HbF v intervalu 2–5 % z celkového Hb; 3. skupina (n = 18), HbF > 5 % z celkového Hb. U těchto vzorků byl zároveň kvantifikován HbF v thalasemickém modu stejného přístroje. Výsledky: Hodnoty HbF mezi 1–2 % spolu nekorelovaly (r = 0,241). Naopak byla nalezena korelace hodnot mezi 2–5 % (r = 0,825; p < 0,001), stejně tak jako hodnot HbF > 5 % (r = 0,889; p < 0,001). Klinický význam mají především hodnoty patologické, tedy hodnoty HbF větší než 2 % z celkového Hb. Takové hodnoty stanovené v modu pro analýzu glykovaného hemoglobinu je možno pokládat za správné. Naopak hodnoty HbF pod 2 % z celkového Hb stanovené v modu pro glykovaný hemoglobin nejsou analyticky správné. Závěr: Závěrem je možné konstatovat, že analytický mod pro stanovení glykovaného hemoglobinu na analyzátoru Tosoh HLC-723 G7 je možné použít také pro presumptivní kombinované HPLC stanovení fetálního hemoglobinu., Objective: Measurement of foetal hemoglobin (HbF) has an important clinical value in the diagnosis as well as in characterization of some Hb structural variants and other hemoglobinopathies. We evaluated presumptive HbF quantification in analytical mode for glycated hemogobin (HbA1c) on Tosoh HLC-723 G7 analyser. Material and Methods: We analysed 63 blood samples and quantified HbF by mode for glycated hemoglobin. The samples were sorted to 3 groups according HbF values: group 1 (n = 24) included HbF values 1–2 % of total hemoglobin (Hb), group 2 (n = 21) included HbF values 2–5 % of total Hb, and group 3 (n = 18) included HbF > 5 % of total Hb. We simultaneously measured HbF in all samples in mode for β-thalassemia determination on the same analyser. Results: The HbF values between 1–2 % obtained by glycated mode did not correlated with results by β-thalassemia mode (r = 0.241). The both HbF values between 2–5 % and HbF values > 5 % of total Hb correlated acceptable with results by β-thalassemia mode (r = 0.825 and 0.889 respectively; p < 0.001). Only pathological HbF values higher than 2 % of total Hb have the clinical significance. These values measured by mode for HbA1c determination is possible to take as correct. On the contrary, all HbF values lower then 2 % of total Hb, measured by mode for HbA1c determination, are not reliable. Conclusion: We conclude that analytical mode for HbA1c determination is also suitable to use for presumptive combined HbF quantification on Tosoh HLC-723 G7 analyser., Jiří Kukačka, E. Klapková, Richard Průša, and Lit. 7
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
30788. Presyncopal cardiac contractility and autonomic activity in young healthy males
- Creator:
- Grasser, E. K., Goswami, N., and Helmut Hinghofer-Szalkay
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, hemodynamika, krevní tlak, physiology, hemodynamics, blood pressure, celkový periferní odpor, impedanční kardiografie, total peripheral resistance, impedance cardiography, sympathetic withdrawal, LBNP (lower body negative pressure), HUT (head-up tilt), 14, and 612
- Language:
- English
- Description:
- We investigated non-invasively cardiac contractility and autonomic nervous activity during presyncopal orthostatic stress induced in healthy humans. A graded orthostatic stress (GOS) paradigm, consisting of head-up tilt (HUT) combined with lower body negative pressure (LBNP) of increasing magnitude, was used to reach a presyncopal end-point in 15 healthy adults. Continuous beat-to-beat hemodynamic and autonomic parameters were recorded. From supine control (C1) to presyncope (PS), total peripheral resistance index (TPRI) decreased from 2300±500 to 1910±320 dyne*s*m²/cm^5 (p=0.004), index of contractility (IC) from 59±14 to 27±6 1000/s (p<0.0001), left ventricular working index (LVWI) from 5.2±1.3 vs. 3.6±0.6 mmHg*L/(min*m²) (p=0.0001) and acceleration index (ACI) from 65±18 vs. 54±15 100/s² (p=0.04). Low frequency variation of diastolic blood pressure (LFnudBP) increased from 51±14 to 67±11 % (p=0.0006) and of systolic blood pressure (LFnusBP) from 50±6 vs. 67±8 % (p<0.0001). High frequency variation of RR-interval (HFms²RRI) decreased from 385±320 to 38±43 ms² (p=0.001). From late GOS (G3) to PS, TPRI decreased from 2540±640 to 1910±320 dyne*s*m²/cm^5 (p=0.003), IC from 35±6 to 27±6 1000/s (p=0.003), LVWI from 4.6±0.9 to 3.6±0.6 mmHg*L/(min/m²) (p=0.003), LFnusBP from 71±8 to 67±8 % (p=0.03), LFmmHg²dBP from 6.6±4.0 to 4.8±2.9 mmHg² (p=0.0001), LFmmHg²sBP from 9.7±7.8 to 7.4±4.8 mmHg² (p=0.01). HFnuRRI increased from 19±8 to 28±13 % (p=0.008). Myocardial contractility indices and parameters of sympathetic activity were reduced in the presyncopal state, while parasympathic activity was increased. This suggests a decrease in cardiac contractility during orthostatically induced presyncope in healthy subjects., E. K. Grasser, N. Goswami, H. Hinghofer-Szalkay., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
30789. Preterm premature rupture of the membranes and genital mycoplasmas
- Creator:
- Kacerovský, Marian
- Type:
- model:article and TEXT
- Language:
- English
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
30790. Pretreatment with propofol restores intestinal epithelial cells integrity disrupted by mast cell degranulation in vitro
- Creator:
- Li, Jinfei, Huang, Jingxia, Zhang, Rui, Lin, Yiquan, Chen, Qianru, and Gan, Xiaoliang
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- intestinal permeability, mast cell, propofol, and PAR-2
- Language:
- English
- Description:
- Propofol has been shown to against intestinal reperfusion injury when treated either before or after ischemia, during which mast cell could be activated. The aim of this study was to evaluate the role of propofol in restoring the intestinal epithelial cells integrity disrupted by mast cell activation or the released tryptase after activation in vitro. We investigated the effect of: (1) tryptase on Caco-2 monolayers in the presence of PAR-2 inhibitor or propofol, (2) mast cell degranulation in a Caco-2/LAD-2 co-culture model in the presence of propofol, and (3) propofol on mast cell degranulation. Epithelial integrity was detected using transepithelial resistance (TER) and permeability to fluorescein isothiocyanate (FITC)-dextran (the apparent permeability coefficient, Papp). The expression of junctional proteins zonula occludens-1 (ZO-1/TJP1) and occludin were determined using western blot analysis and immunofluorescence microscopy. The intracellular levels of reactive oxidative species (ROS) and Ca2+ were measured using flow cytometry. Tryptase directly enhanced intestinal barrier permeability as demonstrated by significant reductions in TER, ZO-1, and occludin protein expression and concomitant increases in Papp. The intestinal barrier integrity was restored by PAR-2 inhibitor but not by propofol. Meanwhile, mast cell degranulation resulted in epithelial integrity disruption in the Caco-2/LAD-2 co-culture model, which was dramatically attenuated by propofol. Mast cell degranulation caused significant increases in intracellular ROS and Ca2+ levels, which were blocked by propofol and NAC. Propofol pretreatment can inhibit mast cell activation via ROS/Ca2+ and restore the intestinal barrier integrity induced by mast cell activation, instead of by tryptase.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public