LED lamps with various combinations of red (R) and blue (B) wavelengths were used to supplement sunlight for the growth of a heat-resistant (HR) and heat-sensitive (HS) recombinant inbred lines (RIL) of lettuce. The RB-LED ratios were 100R:0B (0B), 92R:8B (8B), 84R:16B (16B), and 76R:24B (24B) with an equal PPFD of 100 μmol m-2 s-1. The greatest leaf expansion rates were observed at 8B for both genotypes. All HR-RILs had similar values of growth parameters and specific leaf area (SLA). However, higher values of growth parameters were observed in HS-RIL with 0B, 8B, and 16B than that under 24B and sunlight. Furthermore, HS-RIL had higher SLA under 0B compared to other conditions. Photosynthetic light-use efficiency and maximal oxygen evolution rate were the lowest under 8B for both genotypes. The quality of LED lighting, if provided, seemed to implicate genotype dependence, probably as a result of their different sensitivities to heat stress., T. W. Choong, J. He, L. Qin, S. K. Lee., and Obsahuje bibliografii
In this study, we presented a new approach for quantification of bicarbonate (HCO3-) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and 18O-labelling, excludes the possibility of "non-accounted" HCO3- by avoiding (1) the employment of formate for removal of HCO3- from PSII, and (2) the extremely low concentrations of HCO3-/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3-, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 ± 0.1 HCO3- are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3- molecule as ligand to the non-heme iron of PSII, while unbound HCO3- optimizes the water-splitting reactions by acting as a mobile proton shuttle., K. Tikhonov, D. Shevela, V. V. Klimov, J. Messinger., and Obsahuje bibliografické odkazy
A portable open gas-exchange system (Li-6400, Li-Cor, Inc., Lincoln, NE, USA) has been widely used for the measurement of net gas exchanges and calibration/parameterization of leaf models. Measurement errors due to diffusive leakage rates of water vapor (LW) and CO2 (LC) between inside and outside of the leaf chamber, and the inward dark transpiration rate (DW) and dark respiration rate (DC) released from the leaf under the gasket, can be significant. Rigorous model-based approaches were developed for estimating leakage coefficients of water vapor (KW) and CO2 (KC) and correcting for the combination of these errors. Models were based on mass balance equations and the Dusty Gas Model for a ternary gas mixture of water vapor, CO2, and dry air. Experiments were conducted using two Li-6400 systems with potato and soybean leaves. Results indicated that models were reliable for estimating KW and KC, and the values varied with instrument, chamber size, gasket condition, and leaf structure. A thermally killed leaf should be used for this determination. Measurement error effects on parameterization of the Farquhar et al. (1980) model as determined by PN/C i curves were substantial and each parameter had its own sensitivity to measurement errors. Results also indicated that all four error sources should be accounted for when correcting measurements., Q. Wang ... [et al.]., and Obsahuje bibliografii a dodatky
On the basis of values from literature it was established that photosynthetically used radiation (PUR) amounts to 6 % of absorbed radiant energy in cabbage (producer of high yields), 3.5 % in sugar beet leaves, and 2.6 % in tobacco leaves. PUR of these species did not depend on irradiance in a wide range from 22 to 287 W m-2.
Potential daily biomass production of jutě was calculated from the conversion of measured incident photosynthetic photon flux (PPF) during the jutě growing season (Apríl - August) of Indo-Bangladesh sub-continent and evaluating the average respirátory loss over the same period. Taking an average incident PPF of 39.87 mol(photon) m-2 d’*, the potential daily rate of dry matter production of 49.77 g m'2 was estimated with a photosynthetic efficiency of 1.25 g mol'^ Considering the daily production rate as proportional to the amount of radiant energy interception by the crop at different stages of growth, a maximum jutě biomass production capacity of 4.081 kg m'2 (40.81 t ha'*) was estimated. This value was little more than two times the average production obtained from the field experiment under similar conditions. Radiation use efficiency of a dosed jutě canopy (3.5 g MJ**), however, appears to háve reached that for any field crop.
Chloroplast thylakoid contains several membrane-bound protein kinases that phosphorylate thylakoid polypeptides for the regulation of photosynthesis. Thylakoid protein phosphorylation is activated when the plastoquinone pool is reduced either by light-dependent electron flow through photosystem 2 (PS2) or by adding exogenous reductants such as durohydroquinone in the dark. The major phosphorylated proteins on thylakoid are components of light-harvesting complex 2 (LHC2) and a PS2 associated 9 kDa phosphoprotein. Radiation inactivation technique was employed to determine the functional masses of various kinases for protein phosphorylation in thylakoids. Under the photosynthetically active radiation (PAR), the apparent functional masses of thylakoid protein kinase systems (TPKXs) for catalyzing phosphorylation of LHC2 27 and 25 kDa polypeptides were 540±50 and 454±35 kDa as well as it was 448±23 kDa for PS2 9 kDa protein phosphorylation. Furthermore, the functional sizes of dark-regulated TPKXs for 25 and 9 kDa proteins were 318±25 and 160±8 kDa. The 9 kDa protein phosphorylation was independent of LHC2 polypeptides phosphorylation with regard to its TPKX functional mass. Target size analysis of protein phosphorylation mentioned above indicates that thylakoid contains a group of distinct protein kinase systems. A working model is accordingly proposed to interpret the interaction between these protein kinase systems. and S. C. Lee ... [et al.].
Net photosynthetic rate, radiation use efficiency, chlorophyll (Chl) fluorescence, photochemical reflectance index (PRI), and leaf water potential were measured during a 25-d period of progressive water deficit in quinoa plants grown in a glasshouse in order to examine effects of water stress and ontogeny. All physiological parameters except Fv/Fm were sensitive to water stress. Ontogenic variations did not exist in Fv/Fm and leaf water potential, and were moderate to high in the other parameters. The complete recovery of photosynthetic parameters after re-irrigation was related with the stability in Fv/Fm. PRI showed significant correlation with predawn leaf water potential, Fm', and midday Fv/Fm. Thus PRI and Chl fluorescence may help in assessing physiological changes in quinoa plants across different developmental stages and water status. and T. Winkel, M. Méthy, F. Thénot.
Investigations conceming the deactivation of radiant energy absorbed by the pigments of photosynthetic organisms, either through emitting the fluorescence and delayed luminescence or converting into heat in slow or fast processes, are described. These paths of deactivation can be established by measurements of the absorption, fluorescence excitation, delayed emission and photoacoustic spectra in the same sample. The slow paths of radiative and non-radiative deactivation are of a speciál interest. Even with complex photosynthetic samples it is possible to evaluate slow and fast components of the thermal deactivation from photoacoustic spectra taken at various frequencies of the radiation modulation. In all samples containing reaction centres, anteima complexes or their models, at least a part of delayed deexcitation is due to ionization and delayed recombination of pigments. This is confirmed by photopotential generation for the same samples located in a photoelectrochemical cell. The methods of investigating slow processes of radiative and radiationless deexcitation of the photosynthetic pigments in organisms, their fragments and model Systems are described. Also the results of spectral measurements from some experiments are shown as examples of the described proceduře. These measurements were carried out predominantly as an attempt at explaining the interactions between chlorophylls and carotenoids.