An assessment of DNA integrity in erythrocytes using the alkaline comet assay was carried out to estimate the impacts of water pollution on Balkan loaches (Cobitis elongata Heckel et Kner, 1858) inhabiting the Sava and Kupa Rivers, rivers of varying different water quality. The amount of DNA damage in cells was estimated from three different parameters comet tail length as the extent of genetic material migration, tail intensity (% DNA in comet tail) and tail moment. The results suggest a genotoxicity of the aquatic environment in the Sava River and demonstrated significantly lower levels of DNA damage in fish captured from the Kupa River. This study confirmed that the comet assay, applied to fish erythrocytes, is a useful tool in determining potential genotoxicity of water pollutants. Although a good DNA damage pattern for Balkan loach was obtained, due to its global and regional conservation status, only restricted use of a small number of specimens per sampling site could be permitted.
Based on the examination and quantitative comparison of the approaches used to assess the energy partitioning in photosystem II, the unified method was proposed to calculate the contribution of the components of nonphotochemical quenching. and D. Kornyeyev, C. R. Guadagno, N. D’Ambrosio.
Hydrogen peroxide (H2O2) production in exhaled air was measured in ventilated preterm newborns at 5, 24 and 48 hours after delivery, using originally designed method of exhaled breath condensate (EBC) collection. H2O2 production in expired gas was 812±34 pmol/20 min during the first measurement and then declined to 389±21 at 24 hours and 259±26 pmol/20 min at 48 hours.
While including gradual long-term flooding of individual residual mining pits, the re-vitalization program of the Podkrušnohorská brown-coal basin area has to assure - beside general water quality standards - satisfactory stability level of future lake banks and their wider environment as required for safe usage of future water lakes. General assessment of factors and conditions influencing bank and/or slope stability of future lakes was realized within the frames of the Czech Republic Academy of Sciences grant project. The grant project solutions include a study of exogenous processes on localities where the mining has been terminated. Also, prognoses were developed on behaviour of future banks during flooding and subsequent operation of future lakes. The presented paper is focused on assessment of mayor factors that participate on initiation and intensity of the bank abrasion and sliding deformations., Tamara Spanilá, Zdeněk Kudrna, Vladislav John, Filip Hartvich and Vladimír Chour., and Obsahuje bibliografické odkazy
Twenty-five genotypes of early CIMMYT hexaploid wheat were screened for salt tolerance in a glasshouse experiment using photosynthetic capacity and water relation parameters as selection criteria. Under salt stress (150 mM NaCl) the genotypes Frontana, Norin-10, Mayo-54, Noreste-66, and Yaktana-54 excelled all other lines in shoot dry mass, and Na(20)TPP, Penjamo-62, Inia-66, Frontana, Siete Cerros, and Jaral-66 in grain yield per plant in both absolute and relative (percent of control) terms. Although net photosynthetic rate (PN) declined in all genotypes due to salt stress, it was not helpful in discriminating among genotypes according to salt tolerance. Similarly, no positive relationships of salt tolerance of the genotypes with stomatal conductance, leaf water potential, or turgor pressure were found. Every genotype used its own specific mechanism to tolerate salt stress. However, a large amount of variation in salt tolerance observed in 25 early CIMMYT wheat genotypes can be of considerable practical value for improving salt tolerance in the existing commercial hexaploid wheats. and M. Ashraf, M. Shahbaz.
Performance of 24h static Precise Point Positioning (PPP) solutions based on multi-GNSS precise satellite orbit and clock products from four analysis centers and seven various constellation combinations was studied to evaluate their quality and characteristics. Data from ten European and four Chinese GNSS stations and 152 days long period from year 2020 were processed. Obtained coordinates were firstly compared with those provided by IGS final weekly combined solution. In Europe, the best agreement with this reference product was reached by solutions including Galileo signals, namely by a combination of GPS+GLONASS+Galileo systems with a mean RMS of 11 mm. This situation was different in China where inclusion of Galileo always led to worse results and the best agreement was achieved by a combination of GPS+GLONASS systems. Although product provided by German Research Center for Geosciences (GFZ) could be selected as the best performing over Europe and product by Center for Orbit Determination in Europe (CODE) over China, differences between individual precise products were mostly at a minimal level. Secondly, coordinates repeatability over the processed period was computed in order to assess the positioning stability. In this regard, the lowest values in both horizontal and vertical direction were reached by GPS+GLONASS solutions. From the perspective of precise products, the repeatability results were dependent on the selected constellation where mainly a specific behavior of product from Wuhan University (WUM) for Galileo system was observed., Weiguo Li and Michal Kačmařík., and Obsahuje bibliografii
A method for estimation of elastic wave velocity anisotropy based on ultrasonic sounding data during rock-sample loading was developed. The subject matter of the method is approximation of ultrasonic sounding data by triaxial velocity ellipsoid. The applicability of proposed method was verified on investigation of anisot ropic rock samples. Laboratory loading of migmatite samples was realized under various mutual orientations between acting force direction and rock foliation - perpendicular, parallel and under 45°. P-ve velocity of ultrasound waves was monitored by 8 sensors network. The velocity ellipsoid was computed and changes of sizes and waorientation its main axes during loading were analyzed for separate experiments with regard to loading level. It was found, that independently to mutual orientation between rock foliation and loading direction, the minimum velocity vector turns to perpendicular direction to final rupture plane and maximum velocity vector turns to the plane of final rupture., Matěj Petružálek, Jan Vilhelm, Tomáš Lokajíček and Vladimír Rudajev., and Obsahuje bibliografické odkazy
Photosynthetic parameters including net photosynthetic rate (P N), transpiration rate (E), water-use efficiency (WUE), and stomatal conductance (g s) were studied in indoor C3 plants Philodendron domesticum (Pd), Dracaena fragans (Df), Peperomia obtussifolia (Po), Chlorophytum comosum (Cc), and in a CAM plant, Sansevieria trifasciata (St), exposed to various low temperatures (0, 5, 10, 15, 20, and 25°C). All studied plants survived up to 0°C, but only St and Cc endured, while other plants wilted, when the temperature increased back to room temperature (25°C). The P N declined rapidly with the decrease of temperature in all studied plants. St showed the maximum P N of 11.9 μmol m-2 s-1 at 25°C followed by Cc, Po, Pd, and Df. E also followed a trend almost similar to that of P N. St showed minimum E (0.1 mmol m-2 s-1) as compared to other studied C3 plants at 25°C. The E decreased up to ≈4-fold at 5 and 0°C. Furthermore, a considerable decline in WUE was observed under cold stress in all C3 plants, while St showed maximum WUE. Similarly, the g s also declined gradually with the decrease in the temperature in all plants. Among C3 plants, Pd and Po showed the maximum g s of 0.07 mol m-2 s-1 at 25°C followed by Df and Cc. However, St showed the minimum gs that further decreased up to -4-fold at 0°C. In addition, the content of photosynthetic pigments [chlorophyll a, b, (a+b), and carotenoids] was varying in all studied plants at 0°C. Our findings clearly indicated the best photosynthetic potential of St compared to other studied plants. This species might be recommended for improving air quality in high-altitude closed environments., S. M. Gupta, A. Agarwal, B. Dev, K. Kumar, O. Prakash, M. C. Arya, M. Nasim., and Obsahuje seznam literatury
An expedition was undertaken to French Guiana in search of amphibian parasites. Of the 23 anuran species collected and screened for polystomes, the toad Rhinella margaritifera (Laurenti) was the sole species found to be infected with a polystome, namely Wetapolystoma almae Gray, 1983. Of the two caecilian species collected, a new species of Nanopolystoma du Preez, Huyse et Wilkinson, 2008 was discovered from the urinary bladder of the aquatic caecilian Typhlonectes compressicauda (Duméril et Bibron). The small size of the mature worm, two non-diverticulated caeca of equal length that are non-confluent posteriorly, vitelline follicles in two dense lateral fields, a single follicular testis in the middle of the body, small ovary and a single operculated egg in utero, vaginae present and the caecilian host allowed the identification of the specimen as Nanopolystoma. Larger body size, hamulus length, egg diameter and occurrence in the caecilian family Typhlonectidae distinguishes the new species from the two other known polystomes in Nanopolystoma; thus, the description of Nanopolystoma tinsleyi sp. n. is provided within this paper.