Most species of ants inhabiting the temperate zone overwinter underground, whereas those of the genus Temnothorax remain in nests aboveground. I studied the cost of aboveground overwintering. Workers of Temnothorax crassispinus survived in higher numbers (median = 88%) in artificial nests experimentally buried at a depth of 5 cm than those in nests on the surface (48%) of the soil. The results support the hypothesis that overwintering aboveground could be a consequence of a limited supply of nests and/or the advantage of being able to respond quickly to warm temperatures in spring. Address, Slawomir Mitrus., and Obsahuje seznam literatury
Two new body-colour mutations, albinotic (alb) and cream (cr) are isolated and described for the flightless wing-polymorphic bug, Pyrrhocoris apterus (L.). The body colour of alb mutants is white and that of cr mutants either a light cream or creamy yellow. Both the alb and cr mutations most probably inhibit the biosynthesis of the red pigment in epidermal cells that is responsible for the red-coloured body of wild-type bugs. Although sometimes the body colour of younger larval instars of cr mutants is a slightly darker creamy yellow and that of last larval instars of the alb mutants a slightly whiter greyish, there were no other important developmental changes in the body colour of alb and cr homozygous bugs associated with metamorphosis and post-metamorphic aging of adults. Standard genetic analyses showed that both these mutations are inherited as autosomal recessives and exhibit complete penetrance and uniform expression in both sexes. The fertility of the alb and cr homozygous females and fertilization success of the alb and cr homozygous males were substantially lower than those of wild-type bugs. and Radomír SOCHA.
A total of 345 faecal samples were collected from domestic, captive and wild birds in rural areas, urban areas and a Zoo in Algeria. Samples were screened for the presence of parasites belonging to the genus Cryptosporidium Tyzzer, 1910 by microscopy and PCR analysis of the small-subunit rRNA (SSU), actin and 60-kDa glycoprotein (gp60) genes. Cryptosporidium spp. were detected in 31 samples. Sequence analysis of SSU and actin genes revealed the presence of C. baileyi Current, Upton et Haynes, 1986 in domestic chicken broilers (n = 12), captive ostriches (n = 4) and a wild mallard (n = 1), and C. meleagridis Slavin, 1955 in a graylag goose (n = 1), chickens (n = 11) and turkeys (n = 2). Twenty-three chicken and two turkey broilers from five farms were positive for cryptosporidia, with an overall prevalence of 2% and 6%, respectively. Both C. meleagridis and C. baileyi were detected in farmed chicken broilers, with a prevalence ranging from 9% to 69%. Farmed turkeys broilers were positive only for C. meleagridis, with a 13% prevalence at the animal level. Subtyping of C. meleagridis isolates at the gp60 locus showed the presence of subtype IIIgA22G3R1 in graylag goose and chicken broilers and IIIgA23G2R1 in chicken and turkey broilers. Infection with cryptosporidia was not associated with any clinical diseases. The results of the present study, which provides the first data on the prevalence of Cryptosporidium spp. in wild birds in Africa, demonstrate the presence of human pathogenic C. meleagridis in both domestic and wild birds in Algeria., Abd Elkarim Laatamna, Nikola Holubová, Bohumil Sak, Martin Kváč., and Obsahuje bibliografii
a1_Understanding of the diversity of species of Cryptosporidium Tyzzer, 1910 in tortoises remains incomplete due to the limited number of studies on these hosts. The aim of the present study was to characterise the genetic diversity and biology of cryptosporidia in tortoises of the family Testudinidae Batsch. Faecal samples were individually collected immediately after defecation and were screened for presence of cryptosporidia by microscopy using aniline-carbol-methyl violet staining, and by PCR amplification and sequence analysis targeting the small subunit rRNA (SSU), Cryptosporidium oocyst wall protein (COWP) and actin genes. Out of 387 faecal samples from 16 tortoise species belonging to 11 genera, 10 and 46 were positive for cryptosporidia by microscopy and PCR, respectively. All samples positive by microscopy were also PCR positive. Sequence analysis of amplified genes revealed the presence of the Cryptosporidium tortoise genotype I (n = 22), C. ducismarci Traversa, 2010 (n = 23) and tortoise genotype III (n = 1). Phylogenetic analyses of SSU, COWP and actin gene sequences revealed that Cryptosporidium tortoise genotype I and C. ducismarci are genetically distinct from previously described species of Cryptosporidium. Oocysts of Cryptosporidium tortoise genotype I, measuring 5.8-6.9 µm × 5.3-6.5 µm, are morphologically distinguishable from C. ducismarci, measuring 4.4-5.4 µm × 4.3-5.3 µm. Oocysts of Cryptosporidium tortoise genotype I and C. ducismarci obtained from naturally infected Russian tortoises (Testudo horsfieldii Gray) were infectious for the same tortoise but not for Reeve's turtles (Mauremys reevesii [Gray]), common garter snake (Thamnophis sirtalis [Linnaeus]), zebra finches (Taeniopygia guttata [Vieillot]) and SCID mice (Mus musculus Linnaeus)., a2_The prepatent period was 11 and 6 days post infection (DPI) for Cryptosporidium tortoise genotype I and C. ducismarci, respectively; the patent period was longer than 200 days for both cryptosporidia. Naturally or experimentally infected tortoises showed no clinical signs of disease. Our morphological, genetic, and biological data support the establishment of Cryptosporidium tortoise genotype I as a new species, Cryptosporidium testudinis sp. n., and confirm the validity of C. ducismarci as a separate species of the genus Cryptosporidium., Jana Ježková, Michaela Horčičková, Lenka Hlásková, Bohumil Sak, Dana Květoňová, Jan Novák, Lada Hofmannová, John McEvoy, Martin Kváč., and Obsahuje bibliografii
The mite family Ascouracaridae Gaud et Atyeo, 1976 contains large-sized mites (mostly > 1 mm) which live inside the quills of birds of several orders. To date, no representative of this family has been found associated with the order Strigiformes (owls). In this paper, a new species of this family, Cystoidosoma hermaphroditus sp. n., is described from the tropical screech owl, Megascops choliba (Vieillot) (Aves: Strigiformes) from Brazil. This species is unique in having an external spermaduct, a primary duct and a rudimentary bursa copulatrix present in males. This is the first astigmatan feather mite described from the order Strigiformes in this country. A key to adults of the genus Cystoidosoma Gaud et Atyeo, 1976 of the world is presented., Fabio Akashi Hernandes, Barry M. OConnor., and Obsahuje bibliografii
Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies., Leonela Carabajal Paladino ... [et al.]., and Obsahuje seznam literatury
1_Chromosomes of six European species (one with two subspecies) of Orthoptera belonging to the tribes Ephippigerini and Bradyporini were analyzed using C-banding, Ag-NOR, DAPI (AT-rich)/CMA3 (GC-rich) staining and fluorescence in situ hybridization (FISH) using the 18S rDNA and (TTAGG)n telomeric probes with the aim to better understand chromosomal organization and evolutionary relationships between genera and subgenera within and across both tribes. The evolution of karyotypes was studied in terms of changes in chromosome number (2n) and morphology (FN, the fundamental number – i.e. the number of chromosome arms including the X chromosome). The ancestral 2n = 31 was reduced to 2n = 29 (FN = 31) and 27 (FN = 31) by one or two Robertsonian fusions in the Ephippigerini. Whereas in the Bradyporini 2n = 27 (FN = 32) as a result of two Robertsonian translocations and a pericentric inversion in the X chromosome. The quantity of heterochromatin in GC-rich regions distinguished the karyotypes of Ephippigerini (only a single CG-rich band on one autosome pair) from those of Bradyporini (CG-rich bands on all chromosomes). FISH using the 18S rDNA probe localized 1–3 rDNA clusters to autosomes and/or to the X chromosome in all species examined. The rDNA loci coincided with active NORs as determined by Ag-NOR staining. A comparison of the location of the single NOR/rDNA in two species of the genus Steropleurus (Ephippigerini) suggests that the reduced chromosome number in S. pseudolus results from a Robertsonian fusion between two pairs of autosomes, one of them carrying the NOR/rDNA as in S. stalii (and also in E. ephippiger)., 2_Whereas the karyotypes of three species of the genus Bradyporus, though showing the same chromosome number and morphology, differed in the number and distribution of NORs/rDNA sites [one autosomal in B. (B.) dasypus versus three in B. macrogaster and B. (C.) oniscus, two of them X-linked]. Trends in karyotype diversification of the taxa based on the present data and previous research are discussed. In some individuals belonging to the species Bradyporus (B.) dasypus and B. (C.) m. macrogaster B chromosomes (Bs) were detected: acrocentric (the smallest elements in the complement) and submetacentric (similar to medium-sized autosomes), respectively., Elzbieta Warchalowska-Sliwa ... [et al.]., and Obsahuje seznam literatury
Studies of insect population under field conditions to establish survival rates, longevity and dispersal rates are rare in the literature. These types of studies are important and can be used to inform studies of the effects of landscape composition and configuration on levels of biodiversity. Here the Cantharidae beetle, Rhagonycha fulva is studied under field conditions to derive estimates of daily survival rates for both males and females as well as local dispersal rates. Survival was studied at two sites, one in Wales and another in England, whilst dispersal was examined only at the Welsh site. Beetles were marked using different coloured enamel paints at the mid-point of a 200 m linear transect. The beetles were almost exclusively found (and marked) on common hogweed, Heracleum sphondylium. No difference in survival was found between males and females at both of the sites. The survival rates found at the two sites also did not differ. The overall daily survival rate was 0.771 equating with a median longevity of 4.37 days. Casual observations yielded a small number of marked individuals in excess of 400 m away from the point of marking. Movement of males along the transect differed from females. Marked females were never found far from the point of marking whilst males moved further away with time. This difference in behaviour is discussed in terms of hypothesized insect dispersal behaviour following emergence as mobile adults., Laura E. Rodwell, Jennifer J. Day, Christopher W. Foster, Graham J. Holloway., and Obsahuje bibliografii
Third-stage larvae of the nematode Serpinema trispinosum (Leidy, 1852) were collected from the midgut of four of five species of adult damselflies (Zygoptera) from a non-irrigated restored semipermanent wetland located in Stillwater, Oklahoma, USA. Of the four infected damselfly species, prevalence and mean abundance was highest for the southern spreadwing, Lestes disjunctus australis Walker (10%, 0.2 ± 0.8) and lowest for the familiar bluet, Enallagma civile (Hagen) (2.5%, 0.04 ± 0.3); whereas mean intensities were lowest for the citrine forktail, Ischnura hastata (Say) (1.5 ± 0.5) and the eastern forktail, Ischnura verticalis (Say) (1.0 ± 0). This is the first record of larvae of S. trispinosum from damselflies. Serpinema trispinosum adults have been reported from 18 species of North and Central American freshwater turtles, whereas microcrustaceans such as copepods serve as intermediate hosts and snails, fish and amphibians serve as paratenic hosts in this nematode's life cycle. However, dietary studies of the 18 species of freshwater turtles reported as definitive hosts for S. trispinosum indicate that aquatic insects including damselflies are more commonly reported in turtle diets than are fish or amphibians. Additionally, unlike snails and amphibians, larval damselflies predominantly feed on microcrustaceans, and our observation of S. trispinosum infecting damselflies may reflect the importance of these insects as paratenic hosts. In the present study, we provide new host information and measurements for third-stage larvae of S. trispinosum from damselfly hosts along with measurements for adult male and female S. trispinosum from turtle hosts from Oklahoma, USA., Crystal M. Wiles, Matthew G. Bolek., and Obsahuje bibliografii