The aim of this study was to determine the impact of increased copper contents on selected physiological processes in oneyear-old Pinus sylvestris L. needles from a former German timber storage area in Warcino Forest District, a subject to an environmental quality survey. Samples were collected from the area with the high copper content in the soil. The control area was a nearby pine tree stand showing unimpeded growth. The significant growth inhibition was found in dwarf shoots and whole needles, increased water content, and reduced dry mass were also observed. The chlorophyll content was lowered, while 20% higher electrolyte leakage was found. Chlorophyll a fluorescence indicated only higher values of the nonphotochemical quenching in P. sylvestris from the Cu-site. Significant differences were shown in the rate of gas exchange measured by changes in carbon dioxide or oxygen concentration. The intensity of photosynthesis in needles of P. sylvestris from the Cu-site measured by CO2 uptake was considerably higher than that of oxygen production. The rate of respiration in the needles from the Cu-site measured by the amount of released CO2 was higher only by 15%, while according to O2 consumed, the rate increased by 30% in relation to the control. Our results suggest that the copper accumulation in P. sylvestris needles affected the morphology and physiology of the studied organs., K. Możdżeń, T. Wanic, G. Rut, T. Łaciak, A. Rzepka., and Obsahuje bibliografii
Seedling recruitment is a critical developmental stage in regeneration of plant populations under Mediterranean conditions that strongly depends on water availability. Seed mass and relative growth rate (RGR) may affect the early establishment of seedlings through different physiological processes. Here, we examined the effects of the seed mass and carbon balance on seedling growth under two water regimes in Rhamnus alaternus L. and Rhamnus ludovici-salvatoris, two Mediterranean shrubs, showing a different ability to recruit seedlings. Plant water consumption and biomass accumulation (ΔB) were measured during three periods of the growth in order to estimate water use efficiency (WUE), RGR, and its components. Additionally, net photosynthesis and leaf, stem, and root respiration were measured in plants grown in pots well watered and under progressive drought. Rhamnus alaternus showed the higher seed mass, ΔB, and plant WUE than that of R. ludovici-salvatoris in all periods and water regimes. The higher RGR of R. alaternus was observed during the first and the second period, but the reverse trend was registered during the third period as a consequence of the higher initial biomass of R. alaternus. Also, R. alaternus showed a higher specific leaf area and estimated carbon balance than that of R. ludovicisalvatoris. The observed differences in ΔB, estimated carbon balance, seed mass, and WUE between both species could explain their different distribution and ability to recruit seedlings under natural conditions., H. El Aou-Ouad, I. Florez-Sarasa, M. Ribas-Carbó, J. Flexas, H. Medrano, J. Gulías., and Obsahuje seznam literatury
The present study attempts to determine how some physiological and reproductive functions of olive tree (Olea europaea L., cv. Koroneiki) respond to enhanced UV-B radiation or heat. Enhanced UV-B radiation was applied to (1) three-year-old potted plants in an open nursery (corresponded to ca. 16% ozone depletion), and (2) in vitro cultured pollen samples (220 μmol m-2 s-1, PAR = 400-700 nm + UV-B at 7.5, 15.0, or 22.5 kJ m-2 d-1). Potted olive plants were also subjected to high temperature (38 +- 4°C) for 28 h to mimic heat levels regularly measured in olive growing areas. A significant effect of UV-B on photosynthetic rate was observed. However, enhanced UV-B radiation did affect neither chlorophyll nor carotenoid content, supporting previous reports on hardiness of the photosynthetic apparatus in olive. Increased superoxide dismutase activity was observed in UV-B-treated olive plants (+ 225%), whereas no effect was found in the plants under heat stress. Neither UV-B and nor heat did affect H2O2 accumulation in the plant tissues. However, the same treatments resulted in enhanced lipid peroxidation (+ 18% for UV-B and + 15% for heat), which is likely linked to other reactive oxygen species. The increased guaiacol peroxidase activity observed in both treatments (+ 32% for UV-B and + 49% for heat) is related to the defense against oxidative membrane damage. The observed reduction in pollen germination (20-39%) and tube length (11-44%) could have serious implications on olive yields, especially for low fruit-setting cultivars or in years and environments with additional unfavorable conditions. UV-B and heat effects described here support the hypothesis that plant response to a given stressor is affected by the overall context and that a holistic approach is necessary to determine plant strategies for climate change adaptation., G. C. Koubouris, N. Kavroulakis, I. T. Metzidakis, M. D. Vasilakakis, A. Sofo., and Obsahuje bibliografii
At present, chlorophyll meters are widely used for a quick and nondestructive estimate of chlorophyll (Chl) contents in plant leaves. Chl meters allow to estimate the Chl content in relative units - the Chl index (CI). However, using such meters, one can face a problem of converting CI into absolute values of the pigment content and comparing data acquired with different devices and for different plant species. Many Chl meters (SPAD-502, CL-01, CCM-200) demonstrated a high degree of correlation between the CI and the absolute pigment content. A number of formulas have been deduced for different plant species to convert the CI into the absolute value of the photosynthetic pigment content. However, such data have not been yet acquired for the atLEAF+ Chl meter. The purpose of the present study was to assess the applicability of the atLEAF+ Chl meter for estimating the Chl content. A significant species-specific exponential relationships between the atLEAF value (corresponding to CI) and extractable Chl a, Chl b, Chl (a+b) for Calamus dioicus and Cleistanthus sp. were shown. The correlations between the atLEAF values and the content of Chl a, Chl b, and Chl (a+b) per unit of leaf area was stronger than that per unit of dry leaf mass. The atLEAF value- Chl b correlation was weaker than that of atLEAF value-Chl a and atLEAF value-Chl (a+b) correlations. The influence of light conditions (Chl a/b ratio) on the atLEAF value has been also shown. The obtained results indicated that the atLEAF+ Chl meter is a cheap and convenient tool for a quick nondestructive estimate of the Chl content, if properly calibrated, and can be used for this purpose along with other Chl meters., E. V. Novichonok, A. O. Novichonok, J. A. Kurbatova, E. F. Markovskaya., and Obsahuje seznam literatury
Heat stress is a major production constraint of sunflower worldwide. Therefore, various populations (parental, F1, F2, F3, and plant progenies) of sunflower were screened for leaf gas-exchange traits with the objectives to formulate selection criteria of heat resistance and development of heat-resistant lines. Initial screening and F2 seeds exposed to heat stress (45°C) resulted in the development of an adapted F2 population that showed leaf gas-exchange and morphological traits better than the unadapted population. Correlation coefficients of traits were partitioned into direct and indirect effects via a path analysis technique to determine the cause of their relationship with a basic parameter such as a reproductive head mass (HM). Path analysis showed a positive direct effect of leaf temperature (Tleaf) (0.32) on HM and also an indirect effect (0.77) of the transpiration rate (E) on HM. Moreover, Tleaf showed high heritability estimates. Tleaf was used to select superior plants within the F2 population. This selection brought about an improvement in the net photosynthetic rate (PN) and E as it was indicated from progeny performance and realized heritability. Progenies selected on the basis of Tleaf also showed an increase in achene yield and heat resistance over unselected F3 progenies and a commercial hybrid. and T. Kalyar ... [et al.].
Variations in leaf gas-exchange characteristics, leaf pigment content, and other important leaf traits were investigated in seven wild Oryza species, five hybrids, and five improved varieties. The significant variations were observed in photosynthetic pigment contents amongst different species of Oryza. The mean chlorophyll (Chl) content was higher in O. sativa (varieties and hybrids), while O. eichengeri showed the lowest Chl content. The mean carotenoid (Car) content in O. sativa (varieties and hybrids) was higher than in other wild rice species. O. eichengeri and O. barthii had significantly lower Car contents than other rice species. Significant differences were noticed in the rate of photosynthesis (PN), stomatal conductance (gs), transpiration rate (E), internal CO2 concentration (Ci), specific leaf mass (SLM), and leaf thickness amongst different Oryza species. The mean PN was the highest in O. nivara followed by O. eichengeri. The mean PN was the lowest in O. glumaepatula, which was lower than that of cultivated varieties and hybrids of O. sativa. High rates of photosynthesis were observed in O. nivara (ACC. No. CR 100097), O. rufipogon (ACC.No. CR 100267), and O. nivara (ACC.No. CR 100008). The O. nivara and O. rufipogon genotypes with high PN might be used in rice improvement programmes for an increase of leaf photosynthesis in rice. Multiple correlations performed between different gas-exchange characteristics and other physiological traits revealed that the rate of photosynthesis was not dependent on the leaf pigment content or the leaf thickness. A strong positive correlation between PN and the PN/Ci ratio, which represents the carboxylation efficiency, indicated that the observed variation in PN was not based on pigment content or other leaf traits. and T. V. Kiran ... [et al.].
Variations in leaf gas-exchange characteristics, PSII activity, leaf pigments, and tuber yield were investigated in seven wild and one cultivated species of Dioscorea from Koraput, India, in order to find out their overall adaptability to the environment. The leaf photosynthetic rate, transpiration, stomatal conductance, water-use efficiency, carboxylation efficiency, and photosynthetic pigments were significantly higher in some wild species compared to the cultivated species. In addition, some wild species showed better photochemical efficiency of PSII, photochemical quenching, and electron transport rate in comparison to cultivated one. Furthermore, leaf dry matter accumulation and tuber yield was also higher in some wild species compared to the cultivated species. Taken together, the wild species, such as D. oppositifolia, D. hamiltonii, and D. pubera, showed the superior photosynthetic efficiency compared to the cultivated D. alata and they could be used for future crop improvement programs., B. Padhan, D. Panda., and Obsahuje bibliografii
The present research was conducted to assess physiological responses of ‘Malas-e-Saveh’ (Malas) and ‘Shishe-Kab’ (Shishe) pomegranates to water of different salt content and electrical conductivity (1.05, 4.61, and 7.46 dS m-1). Both cultivars showed a reduced trunk length due to salinity. Relative water content and stomatal conductivity of both cultivars were significantly reduced under salt stress, but ion leakage increased. In both cultivars, total chlorophyll (Chl) and carbohydrates decreased with rise in salinity, while proline accumulation increased. With salinity increment, the Chl fluorescence parameters (maximum photochemical efficiency of PSII and effective quantum yield of PSII) declined significantly in both cultivars, with higher reduction observed in Shishe. Generally, more Na+ accumulated in shoots and more Cl- was observed in leaves. Cl- accumulation increased by salinity in leaves of Malas, but it was reduced in Shishe. The K+/Na+ ratio in leaves decreased in both cultivars by salinity increment. Malas was less affected by osmotic effects of NaCl, but it accumulated more Cl- in its leaves. Thus, Malas might be more affected by negative effects of salinity., M. Khayyat, A. Tehranifar, G. H. Davarynejad, M. H. Sayyari-Zahan., and Obsahuje bibliografii
Among various epiphytic ferns found in the Brazilian Atlantic Forest, we studied Vittaria lineata (L.) Smith (Polypodiopsida, Pteridaceae). Anatomical characterization of the leaf was carried out by light microscopy, fluorescence microscopy, and scanning electron microscopy. V. lineata possesses succulent leaves with two longitudinal furrows on the abaxial surface. We observed abundant stomata inside the furrows, glandular trichomes, paraphises, and sporangia. We examined malate concentrations in leaves, relative water content (RWC), photosynthetic pigments, and chlorophyll (Chl) a fluorescence in control, water-deficient, and abscisic acid (ABA)-treated plants. Plants subjected to drought stress (DS) and treated by exogenous ABA showed significant increase in the malate concentration, demonstrating nocturnal acidification. These findings suggest that V. lineata could change its mode of carbon fixation from C3 to the CAM pathway in response to drought. No significant changes in RWC were observed among treatments. Moreover, although plants subjected to stress treatments showed a significant decline in the contents of Chl a and b, the concentrations of carotenoids were stable. Photosynthetic parameters obtained from rapid light curves showed a significant decrease after DS and ABA treatments., B. D. Minardi, A. P. L. Voytena, M. Santos, Á. M. Randi., and Obsahuje bibliografii
Responses to drought were studied using two maize inbred lines (B76 and B106) and a commercial maize hybrid (Zea mays L. cv. Silver Queen) with differing resistance to abiotic stress. Maize seedlings were grown in pots in controlled environment chambers for 17 days and watering was withheld from one half the plants for an additional 11 days. On the final treatment date, leaf water potentials did not differ among genotypes and were -0.84 and -1.49 MPa in the water sufficient and insufficient treatments, respectively. Greater rates of CO2 assimilation were retained by the stress tolerant maize inbred line, B76, in comparison to the other two genotypes 11 days after watering was withheld. Rates of CO2 assimilation for all three genotypes were unaffected by decreasing the measurement O2 concentration from 21 to 2% (v/v). Activities of phosphoenolpyruvate carboxylase (PEPC), NADP-malic enzyme (NADP-ME), and NADP malate dehydrogenase were inhibited from 25 to 49% by the water deficiency treatment. Genotypic differences also were detected for the activities of NADP-ME and for PEPC. Changes of transcript abundance for the three C4 pathway enzymes also varied among watering treatments and genotypes. However, examples where transcripts decreased due to drought were associated with the two stress susceptible genotypes. The above results showed that enzymes in the C4 photosynthetic pathway were less inhibited by drought in stress tolerant compared to stress susceptible maize genotypes., R. Sicher, J. Bunce, J. Barnaby, B. Bailey., and Obsahuje bibliografii