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2. Intestinal parasites of buffalo calves from Romania: molecular characterisation of Cryptosporidium spp. and Giardia duodenalis, and the first report of Eimeria bareillyi

Creator:
Bărburaș, Diana Ancuța, Cozma, Vasile, Ionică, Angela Monica, Abbas, Ibrahim, Bărburaș, Remus, Mircean, Viorica, D'Amico, Gianluca, Dubey, Jitender P., and Györke, Adriana
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
buffaloes, parasites, Toxocara vitulorum, Eimeria bareillyi, Cryptosporidium, and Giardia
Language:
English
Description:
Buffaloes represent an important economic resource for several regions of the world including Romania. In the present study, we examined 104 faecal samples collected from 38 buffalo calves (2-11 weeks old) from household rearing systems in Romania for gastrointestinal parasites. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with sequencing isolates were used to identify assemblages of Giardia lamblia (Kunstler, 1882) and species of Cryptosporidium Tyzzer, 1907. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites: 16 had single infections and 17 had mixed infections with two or three parasites. Species of Eimeria Schneider, 1875 (32/38; 84%) were the most prevalent parasites; eight species were identified according to the oocyst morphology, including the pathogenic E. bareillyi (Gill, Chhabra et Lall, 1963) which was detected for the first time in buffaloes from Romania. The nematodes Toxocara vitulorum (Goeze, 1782) (11/38; 37%) and Strongyloides papillosus (Wedl, 1856) (6/38; 16%) were also detected. Cryptosporidium spp. were found in four (11%) buffalo calves; two of them were molecularly identified as C. ryanae Fayer, Santin et Trout, 2008, and another one clustered in the same clade with C. ryanae, C. bovis Fayer, Santin et Xiao, 2005, and C. xiaoi Fayer et Santin, 2009. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Multilocus genotyping of Giardia duodenalis isolates from red deer (Cervus elaphus) and roe deer (Capreolus capreolus) from Poland

Creator:
Solarczyk, Piotr, Majewska, Anna C., Moskwa, Bożena, Cabaj, Władysław, Dabert, Miroslawa, and Nowosad, Piotr
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Giardia, genotypes, molecular epidemiology, phylogeny, and zoonoses
Language:
English
Description:
A total of 181 faecal samples were collected from wild cervids in two regions of Poland. Giardia cysts were detected in one faecal specimen from red deer and in two samples from roe deer. Fragments of the β-giardin (bg) triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified from the Giardia isolate obtained from red deer, whereas only amplicons of bg and gdh were obtained from Giardia isolates derived from two roe deer. The result of genotyping and phylogenetic analysis showed that the G. duodenalis isolate from red deer belonged to sub-assemblage AIII, which has never been identified in humans, whereas isolates from roe deer clustered within zoonotic sub-assemblage AI. Further studies are necessary to explain which Giardia assemblages and/or sub-assemblages occur in wild cervids in various regions of the world. Moreover, the impact of Giardia infection on the health of wild cervids should also be elucidated.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Stray cats are more frequently infected with zoonotic protists than pet cats

Creator:
Martin Kváč, Hofmannová, Lada, Ynes R Ortega, Holubová, Nikola, Horčičková, Michaela, Kicia, Marta, McEvoy, John, Lenka Hlásková, Dana Květoňová, and Bohumil Sak
Format:
print, počítač, and online zdroj
Type:
model:article and TEXT
Subject:
Giardia, antiparazitika, antiparasitics, Cryptosporidium, Encephalitozoon, Enterocytozoon bieneusi, PCR, 2, and 59
Language:
English
Description:
Faecal samples were collected from cats kept as pets (n = 120) and stray cats (n = 135) in Central Europe (Czech Republic, Poland and Slovakia) and screened for the presence of Cryptosporidium spp., Giardia intestinalis (Kunstler, 1882), Encephalitozoon spp. and Enterocytozoon bieneusi Desportes, Le Charpentier, Galian, Bernard, Cochand-Priollet, Lavergne, Ravisse et Modigliani, 1985 by PCR analysis of the small-subunit of rRNA (Cryptosporidium spp. and G. intestinalis) and ITS (microsporidia) genes. Sequence analysis of targeted genes revealed the presence of C. felis Iseki, 1979, G. intestinalis assemblage F, E. cuniculi Levaditi, Nicolau et Schoen, 1923 genotype II, and E. bieneusi genotype D. There was no correlation between the occurrence of detected parasites and sex, presence of diarrhoea or drug treatment (drug containing pyrantel and praziquantel). Compared to pet cats (7%), stray cats (30%) were statistically more frequently infected with protist parasites and overall may present a greater risk to human health., Martin Kváč, Lada Hofmannová, Ynes Ortega, Nikola Holubová, Michaela Horčičková, Marta Kicia, Lenka Hlásková, Dana Květoňová, Bohumil Sak, John McEvoy., and Obsahuje bibliografii
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public