Cobitis elongata and Sabanejewia balcanica from the family Cobitidae were collected monthly from September 2004 to October 2005 from the Petrinjčica River in the Danube basin of Croatia. On each sampling date, samples of the macrozoobenthos were taken from three different substrates. The density of the macrozoobenthos was calculated and compared with the diet of the loaches. During all months the most dominant group in the macrozoobenthos and in the guts of the analysed loaches was insect larvae of the family Chironomidae. Ivlev’s index was used as a measure of selectivity for various macroinvertebrate taxa in the fish rations and showed changing selectivity that indicate opportunistic feeding strategies and wide diet overlaps between the species.