The objective of this work was to determine the concentrations of four heavy metals (Pb, Zn, Fe and Cu) in muscle and liver from the solitary subterranean rodent Ctenomys talarum from natural dunes, cultivated area and military area with the purpose of assessing their levels and their possible effect on the biology of this small mammal. Only Pb in tissues of C. talarum from the agricultural zone indicates a risk of toxic effects on the organism. Overall concentrations of heavy metals in soils and tissues of C. talarum from all areas are consistent with lightly polluted habitats, although the increments observed in areas under anthropic use suggest for the possibility that carrying out these human activities for long periods of time, or in an intensive way, could exert an evident impact on ecosystems.