RAPD-PCR method is now widely employed in genetic research and is a powerful asset when trying to identify species. In the present work, RAPD genetic markers were obtained for distinguishing between three species of the genus Gobio that show substantial morphological resemblance. Different profiles were observed: six species-specific markers were detected for G. gobio, four for G. kesslerii and nine for G. uranoscopus. These markers were reproducible, dependable and very helpful in differentiating between these species.