The aim of the study was to examine the potential impacts of bisphenol A (BPA) and its analogues BPB, BPF, and BPS on mice TM3 Leydig cells, with respect to basal cell viability parameters such as metabolic activity, cell membrane integrity, and lysosomal activity after 48-h exposure. In addition, monitoring of potential bisphenol´s actions included evaluation of ROS production and gap junctional intercellular communication (GJIC) complemented by determination of testosterone secretion. Obtained results revealed significant inhibition in mitochondrial activity started at 10 µg/ml of bisphenols after 48-h exposure. Cell membrane integrity was significantly decreased at 5 µg/ml of BPA and BPF and 10, 25, and 50 µg/ml of BPA and BPS. The lysosomal activity was significantly affected at 10, 25, and 50 µg/ml of applied bisphenols. A significant overproduction of ROS was recorded mainly at 5 and 10 µg/ml of tested compounds. In addition, significant inhibition of GJIC was observed at 5 µg/ml of BPB followed by a progressive decline at higher applied doses. In the case of testosterone production, a significant decline was confirmed at 10, 25 and 50 µg/ml.