Molekulární metody umožnily studovat šíření rostlin v povodí Labe. Druhy šířené vodou (zevar, stulík) migrují především jednosměrně podél řek. Bylo prokázáno i šíření mezi říčními systémy, častější je u druhů šířících se také větrem (rákos, orobinec). Klonální šíření mezi populacemi není u těchto studovaných druhů rostlin tak běžné, jak se dosud předpokládalo., Molecular approaches have allowed for the study of plant dispersal in the catchment area of the River Elbe. Plants dispersed by water (Bur-reed, Pond Lily) migrated mainly unidirectionally along streams. Dispersal among river systems was also detected and is more common among plants with wind dispersal (Common Reed, Cattail). Clonal dispersal does not seem to be as common in the studied species as previously suggested., and Tomáš Fér.
Statin-associated myopathy (SAM) represents a broad spectrum of disorders from insignificant myalgia to fatal rhabdomyolysis. Its frequency ranges from 1-5 % in clinical trials to 15-20 % in everyday clinical practice. To a large extent, these variations can be explained by the definition used. Thus, we propose a scoring system to classify statin-induced myopathy according to clinical and biochemical criteria as 1) possible, 2) probable or 3) definite. The etiology of this disorder remains poorly understood. Most probably, an underlying genetic cause is necessary for overt SAM to develop. Variants in a few gene groups that encode proteins involved in: i) statin metabolism and distribution (e.g. membrane transporters and enzymes; OATP1B1, ABCA1, MRP, CYP3A4), ii) coenzyme Q10 production (e.g. COQ10A and B), iii) energy metabolism of muscle tissue (e.g. PYGM, GAA, CPT2) and several others have been proposed as candidates which can predispose to SAM. Pharmacological properties of individual statin molecules (e.g. lipophilicity, excretion pathways) and patients´ characteristics influence the likelihood of SAM development. This review summarizes current data as well as our own results., M. Vrablik, L. Zlatohlavek, T. Stulc, V. Adamkova, M. Prusikova, L. Schwarzova, J. A. Hubacek, R. Ceska., and Obsahuje bibliografii
Template - immunological concept in the genetics' services. On immunological theories in the 1st half of the 20th century and their influence on the terminology of molecular genetics.
To understand the pathogenesis of hypercholesterolemia in Prague hereditary hypercholesterolemic (PHHC) rat, we analyzed the response of hepatic transcriptome to dietary cholesterol in PHHC and control Wistar rats. Male PHHC and Wistar rats were fed chow (C), 5 % fat (palm kernel oil) (CF) or 1 % cholesterol + 5 % fat (CHOL) diet for three weeks. Hepatic transcriptome was analyzed using Affymetrix GeneChip arrays. No differences were found in the effect of both control diets (C and CF) on lipid metabolism and gene expression of 6500 genes. Therefore, these data were pooled for further analysis. Dietary cholesterol induced accumulation of cholesterol and triacylglycerols in the liver in both strains and hypercholesterolemia in PHHC rats. However, there were no differences in response of hepatic transcriptome to CHOL diet. On the other hand, several genes were found to be differently expressed between both strains independently of the diet. Two of those genes, Apof and Aldh1a7, were studied in more detail, and their role in pathogenesis of hypercholesterolemia in PHHC rats could not been corroborated. In conclusion, the hypercholesterolemia in PHHC rats is due to physiological response of hepatic transcriptome to dietary cholesterol in different genetic background., M. Vlachová, M. Heczková, M. Jirsa, R. Poledne, J. Kovář., and Obsahuje bibliografii
Přes obrovský pokrok v molekulárních fylogenetických metodách a množství v nedávné době objevených fosilních taxonů nemáme o fylogenezi a evoluci mravenců (čeleď Formicidae) mnoho ověřených faktů. V současnosti se rozlišuje 16 žijících a tři fosilní podčeledi. Podčeleď Leptanillinae je považována za bazální, zatímco skupina Apoidea (včely a kutilky) se ukazuje jako sesterská k čeledi mravenců., In contrast to the huge progress of molecular phylogenetic methods and many recently discovered fossil taxa, the phylogeny and evolution of ants (Formicidae) remains poorly understood. Today 16 extant and three fossil ant subfamilies are known. The subfamily Leptanillinae is considered as basal, while Apoidea seems to be a sister family to the ants., and Pavel Pech.