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1. Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Creator:
Dyková, Iva, Pindová, Zuzana, Fiala, Ivan, Dvořáková, Helena, and Macháčková, Blanka
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
amphizoic amoebae, Hartmannella vermiformis, morphology, phylogeny, and in situ hybridisation
Language:
English
Description:
Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Ultrastructure and localisation of late-sporogonic developmental stages of Sphaerospora ranae (Myxosporea: Sphaerosporidae)

Creator:
Jirků, Miloslav and Bartošová-Sojková, Pavla
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Amphibia, Anura, Europe, kidneys, myxospore, Myxozoa, Rana, sporogony, synchronisation, and in situ hybridisation
Language:
English
Description:
Data on external ultrastructure of myxospores and internal ultrastructure of advanced pseudoplasmodia and myxospores of topotypic samples of Sphaerospora ranae (Morelle, 1929) from Rana dalmatina Bonaparte are provided, together with in situ hybridisation results. In both frogs examined, the infection was restricted to renal tubules and corpuscles. The infection site restriction was confirmed by light and transmission electron microscopy, as well as by in situ hybridisation. In addition, large myxospore masses measuring up to 500 μm were detected in seminal vesicles. Only late-sporogonic stages, i.e. pseudoplasmodia harbouring immature and/or mature myxospores, were observed and analysed. Scanning electron microscopy revealed that spores have smooth surface with exception of posterior valvular bulges, which possess numerous outwards opening internal canals. As revealed by both scanning and transmission electron microscopy, the canals are continuous invaginations of the outer spore surface. Myxospores of S. ranae are characterised by the presence of two uninucleate sporoplasms, bilayered polar capsules, S/H-shaped polar filaments in transversal section and multilayered polar filament eversion pole plugging complex. Ultrastructural observations are discussed in the context of available data for other species of Sphaerospora sensu stricto and apparent synchronisation of myxospore shedding with a brief aquatic breeding phase of vertebrate intermediate host is highlighted.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public