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2. Effect of cisplatin, carboplatin and stobadine on lipid peroxidation of kidney homogenate and phosphatidylcholine liposomes
- Creator:
- Gergeľ, O., Mišík, V., and Ondriaš, K.
- Type:
- article, model:article, and TEXT
- Subject:
- cisplatin, carboplatin, stobadine, kidney Homogenate, liposomes, and lipid peroxidation
- Language:
- English
- Description:
- The effects of the nephrotoxic, anticancer agents cisplatin (CDI)P) and carboplatin (CBDCA), and the free radical scavenger, stobadine, were investigated on lipid peroxidation (EI’O) of rat kidney homogenates and phosphatidylcholine (PC) liposomes. Kidney homogenates were incubated in air at 37 °C for 6-48 h and lipid peroxidation was detected spectroscopically as absorbance (533 nm) of the thiobarbituric acid- malondialdchyde (TBA-MDA) complex. CDDP (0.3-10 mmol.I'1) increased LPO of the homogenate. CBDCA decreased the TBA-MDA absorbance, yet was found to interfere with MDA, TBA and/or with the TBA-MDA complex. Thus when CBDCA is involved, the TBA- MDA method for detection of LPO is not suitable. Stobadine (0.1 mmol.I'1 ar>d 1 mmol.I1) inhibited LPO either in the control homogenate and in the homogenate where peroxidation was increased by CDDP. The effect of CDDP and CBDCA on peroxidation of PC liposomes was monitored as oxygen consumption using a Clark-type oxygen electrode. CDDP increased but CBDCA decreaed the rate of oxygen consumption during the peroxidation of liposomes induced by FcSO,». The results suggest that the effects of CDDP and CBDCA on LPO may be linked with their nephrotoxicity.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Štúdium interakcie cytochrómu c s kalixarénmi zabudovanými do lipidových membrán
- Creator:
- Hianik, Tibor, Garaiová, Zuzana, Vargová, Veronika, and Vitovič, Pavol
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- cytochromy, lipozomy, biofyzika, cytochromes, liposomes, biophysics, 6, and 53
- Language:
- Czech and English
- Description:
- We studied the interaction of cytochrome c (cyt c) with unilamellar liposomes (ULL) composed of dimyristoylphosphatidylcholine (DMPC) modified by calix[6]arene (CX). We also analyzed the influence of cyt c on the properties of monomolecular films on an air-water interface composed of CX. The changes of the liposome size have been studied by dynamic light scattering and the zeta potential was determined by Doppler velocimetry. By ultrasound attenuation coefficient we also studied the effect of CX on the phase transition of the lipid bilayer of liposomes. The liposomes containing 10mol% CX have been on average 1.5 times larger in comparison with unmodified ULL. The increase of ULL diameter has been accompanied by a decrease of zeta potential toward negative values. This has been due to negatively charged carboxyl groups of CX polar part. The interaction of cyt c with CX resulted in an increase of zeta potential. CX did not affect the phase transition temperature of liposomes (T = 24.4 °C). However, the half width of the transition peak increased which is evidence of a decrease of the cooperativity of phase transition. CX forms stable monomolecular films on the air-water interphase. The presence of cyt c in a water subphase caused an increase of mean molecular area of CX. The dependence of relative changes of the area per molecule as a function of cyt c concentration had the shape of Langmuir isotherm. The dissociation constant, KD, determined in a solid state of the monolayer was 26.0 ± 5.4 nM, which is evidence of good affinity of cyt c to CX., Tibor Hianik, Zuzana Garaiová, Veronika Vargová, Pavol Vitovič., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public