The work deals with design and implementation of an analogue electro-optic transmission system convenient to solve some specialized tasks in the field of diagnostics and measurements. The proposed system is designed to solve several requirements that are beyond the limits of conventional metallic transmission medium. Especially it is the case of broadband transmission, extreme length of the transmission medium and high galvanic barrier between input and output that have to be achieved simultaneously. and Práce popisuje vývoj a realizaci analogového elektro-optického přenosového systému použitelného v některých speciálních případech diagnostiky a měření. Navržený přenosový systém řeší několik vzájemně se limitujících požadavků, které není možné standardním metalickým propojením splnit. Jde zejména o současné dosažení velké šířky frekvenčního pásma, délky přenosového média a dostatečné galvanické bariéry mezi vstupem a výstupem.
Metoda fázově modulační časově rozlišené konfokální mikrospektrofluorimetrie byla využita v kombinaci s fluorescenčním zobrazováním k monitorování transportu fluorescenčně značeného terapeutického oligonukleotidu do buněk za pomoci kationického porfyrinu. Bylo možné sledovat stabilitu vytvořeného transportního komplexu ve vnitrobuněčném prostředí a interakci transportovaného oligonukleotidu s cílovými molekulárními strukturami v buněčném jádře., Time-resolved phase modulation microspectrofluorimetry in combination with fluorescence microscopy imaging have been applied to monitor the cellular uptake fluorescent-labeled therapeutic oligonucleotide delivered by a cationic porphyrin. Transport complex stability inside the cell and the oligonucleotide interaction with the target molecular structures in the cell nucleus has been monitored., Petr Praus, Eva Kočišová, Josef Štěpánek, Peter Mojzeš., and Obsahuje bibliografii
The aim of this study is to explore the sources of attitude constraints regarding the role of government in the economy, and to find out whether the sources of these constraints are the same as in Western democracies. Use is made of Converse’s approach to conceptualize attitude constraint where an individual’s belief system is seen to be a configuration of attitudes and values characterized by a functional interdependence, or constraint. This constraint may be interpreted in terms of the probability of being able to predict one attitude having knowledge of another. In this study, there is a review of the sources of attitude constraint and related measurement issues. Using ISSP 2006 (Role of Government module) an analysis of attitudinal constraints is presented using two attitudinal scales. This research confirms that the sources of attitude constraint in the Czech Republic are similar to those observed in Western Europe and the USA. Specifically, class, education, and other social-demographic variables are shown to have very limited effects. Moreover, Converse’s contention that attitude constraints are strongly determined by political involvement, political knowledge, or party identification is also shown to be valid for Czech society., Lukáš Linek., 1 obrázek, 4 tabulky, Obsahuje bibliografii, and Anglické resumé
This article explores how aggregate level data may be used to make inferences about individual level behaviour. A common strategy in the past was to assume that the relations evident in aggregated data are also present in individual data. Analysis of datasets where there is both individual and aggregated information demonstrates that this assumption is most often incorrect. This means that the relationships observed between variables at an aggregated level are unlikely to be observed in individual level data. This is a problem because quite often social scientists only have aggregated data for exploring individual level behaviour. A key question explored in this article is how is it possible to validly and reliably use aggregated datasets to make inferences about relationships between variables at the individual level. An example analysis is given using electoral data from the Czech Republic., Pat Lyons., 4 obrázky, 4 tabulky, Obsahuje bibliografii, and Anglické resumé