The genus Trichoderma acts as an important antagonist against phytopathogenic fungi. This paper proposes a software-based identification tool for recognition of different species of Trichoderma. The method uses the morphological features for identification. Morphological-based species recognition is common method for identifying fungi, but regarding the similarity of morphological features among different species, their manual identification is difficult, time-consuming and may bring about faulty results. In this paper it is intended to identify different species of Trichoderma by means of neural network. For this purpose, 14 characteristics are used including 5 macroscopic and 9 microscopic characteristics. After quantifying qualitative features and training a multilayer perceptron neural network with quantified data, 25 species of Trichoderma are recognized by using the network. Totally, identification of Trichoderma species as one useful fungus is achieved by using the trained network.