Search

Start Over Subject reprogramming

1. Jak a proč změnit identitu buňky

Creator:
Jiří Klíma, Lišková, Irena, and Jan Motlík
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Biologické vědy, cytogenetika, identita, buňky, cytogenetics, identity, cells, identita buňky, přeprogramování buněk, identity of the cell, reprogramming, 2, and 57/59
Language:
Czech
Description:
Cílená změna původní identity buňky v identitu jinou neboli reprogramování buněk nachází své uplatnění jak v základním výzkumu, tak i v případné buněčné terapii. Z mnoha postupů reprogramování autoři představují přípravu indukovaných pluripotentních buněk (iPS), transdiferencovaných anebo přímo reprogramovaných buněk. Je popsána cesta přípravy a z ní plynoucí vlastnosti a potenciál indukovaných buněk., Induced change in the identity of the cell known as reprogramming of the somatic cell is applicable both in basic research and cell therapy. Out of many reprogramming approaches the authors introduce the derivation of induced pluripotent stem (iPS), transdifferentiated and direct reprogrammed cells. The workflow for the preparation of induced cells and a comment on their properties and potentials are given., and Jiří Klíma, Irena Lišková, Jan Motlík.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Reprogramming of human pancreatic organoid cells into insulin-producing β-like cells by small molecules and in vitro transcribed modified mRNA encoding neurogenin 3 transcription factor

Creator:
Koblas, T., Leontovyc, I., Loukotova, S., and Saudek, F.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
diabetes, organoid, reprogramming, synthetic mRNA, in vitro transcription, IVT, neurogenin 3, β-cell, insulin, TGF, RepSox, and small molecules
Language:
English
Description:
Reprogramming of non-endocrine pancreatic cells into insulin-producing cells represents a promising therapeutic approach for the restoration of endogenous insulin production in diabetic patients. In this paper, we report that human organoid cells derived from the pancreatic tissue can be reprogrammed into the insulin-producing cells (IPCs) by the combination of in vitro transcribed modified mRNA encoding transcription factor neurogenin 3 and small molecules modulating the epigenetic state and signalling pathways. Upon the reprogramming, IPCs formed 4.6 ± 1.2 % of the total cells and expressed typical markers (insulin, glucokinase, ABCC8, KCNJ11, SLC2A2, SLC30A8) and transcription factors (PDX1, NEUROD1, MAFA, NKX2.2, NKX6.1, PAX4, PAX6) needed for the proper function of pancreatic β-cells. Additionally, we have revealed a positive effect of ALK5 inhibitor RepSox on the overall reprogramming efficiency. However, the reprogrammed IPCs possessed only a partial insulin-secretory capacity, as they were not able to respond to the changes in the extracellular glucose concentration by increasing insulin secretion. Based on the achieved results we conclude that due to the incomplete reprogramming, the IPCs have immature character and only partial properties of native human β-cells. and Corresponding author: Tomas Koblas
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public