The karyotypes of one mud loach and three spined loach species occurring in the Far East region of Russia are presented. Misgurnus nikolskyi has 2n=50 with NF=64, Cobitis lutheri has 2n=50 and NF=70, C. choii has 2n=50 and NF=68, and C. melanoleuca has 2n=50 and NF=72. The karyotype of M. mohoity is proved to consist of 50 chromosomes. These results are discussed in relation with some taxonomic and evolution problems in loaches