The term cellular immune response refers to haemocyte-mediated responses, including phagocytosis, nodulation, and encapsulation. In the present study, we identified five types of circulating haemocytes in larvae of the haemolymph of the Asian corn borer, Ostrinia furnacalis (Guenée), including granulocytes, oenocytoids, plasmatocytes, prohaemocytes, and spherulocytes. The relative number of total free haemocytes per larva decreased significantly 0.5, 24, and 36 h after the injection of Beauveria bassiana conidia. Upon conidia challenge, both phagocytosis and nodulation were observed in the collected haemolymph from O. furnacalis larvae. In addition, plasma was found to be necessary for both phagocytosis and nodulation. Therefore, we here confirm that phagocytosis and nodulation are involved in O. funacalis larvae during their fight against infection by B. bassiana, and further, that the cellular immune response of O. furnacalis helps eliminate the invading organisms despite the fact that not all the fungal conidia are killed., Dongxu Shen, Miao Li, Yuan Chu, Minglin Lang, Chunju An., and Obsahuje bibliografii
Facultative diapause in the wax moth, Galleria mellonella, occurs in the final larval instar. Application of juvenile hormone analogs (JHAs) to the larvae of this species has similar effects to diapause, in terms of prolonged development of the larval stages and the arrest in the metamorphosis of internal organs. Here, we focus on testes development and spermatogenesis at the end of larval development in G. mellonella, how they are affected by diapause induced by an environmental decrease in temperature to 18°C and the application of a JHA (fenoxycarb) to larvae. Because neither testis development nor spermatogenesis are described in detail for this species, we examined them in individuals not in diapause during the period from the last larval instar to the newly emerged adult and present a timetable of changes that occur in the development of testes in this species. These observations have increased the very limited data on the course of spermatogenesis in pyralid insects. We then used these data for comparative analysis of testes in larvae from two experimental groups: individuals in diapause and those treated with fenoxycarb. The results on the general morphology testes revealed obvious degenerative changes caused by fenoxycarb (but not by diapause), including testicular wall hypertrophy and disarrangement of testicular follicles. Moreover, treatment with fenoxycarb finally resulted in the disintegration of nearly all testicular cyst-containing germ cells at different stages of spermatogenesis, a situation never previously described in the literature. In contrast, the main effect of diapause on testes was merely the degeneration of spermatocytes in the proximal regions of the testicular follicles. Finally, the TUNEL analyses, revealed that the degenerative changes in germ cells were apoptotic in character in the testes of both individuals in diapause and fenoxycarb-treated males., Piotr Bebas, Bronislaw Cymborowski, Michalina Kazek, Marta Anna Polanska., and Obsahuje bibliografii
Galleria mellonella larvae spin protective tubes, which they use until they finish feeding, when they spin cocoons. A feeding choice experiment showed that some of the silk produced by feeding larvae was consumed in addition to the standard diet (STD). To determine the effect of feeding on silk, last instar larvae were fed for 24 h on foods based on STD but modified by replacing the dry milk component (10% of the diet) with equal amounts of different kinds of silk. While each control larva consumed 21 ± 0.5 mg of the STD and produced 4.5 ± 0.1 mg of silk, larvae that ate the food that contained larval silk consumed 10 ± 0.4 mg of food and produced 6.1 ± 0.1 mg silk; the percentage ratio of silk produced to diet consumed was 21% and 61%, respectively. A more pronounced reduction in food consumption occurred when larvae were supplied with Galleria "cocoon" silk or the sericin fraction of such silk, and only 3.8 mg/larva was ingested of the diet containing Bombyx mori cocoon silk or its sericin fraction. Silk production expressed in terms of percentage of diet consumed was always higher than that recorded for larvae fed STD. We conclude that G. mellonella larvae recycle part of the silk that they produce during feeding. Presence of silk in the diet reduces food intake but increases the ratio of silk production to diet consumption. Sericin fraction of the cocoon silk seems to deter feeding., Haq Abdul Shaik, Archana Mishra, František Sehnal., and Obsahuje bibliografii
The Mediterranean flour moth, Ephestia kuehniella is a widespread pest of stored products and a classical object in experimental biology. In the present study, we determined its complete mitochondrial genome sequence. The genome is circular, consists of 15,327 bp and comprises 13 protein-coding, 2 rRNA- and 22 tRNA-coding genes in an order typical for the Ditrysia clade of the order Lepidoptera. A phylogenetic study of the Lepidoptera based on complete mitochondrial genomes places E. kuehniella correctly in the family Pyralidae and supports major lepidopteran taxa as phylogenetic clades. The W chromosome of E. kuehniella is an exceptionally rich reservoir of originally mitochondrial sequences (numts). Around 0.7% of the W DNA was found to be of mitochondrial origin, 83% of the mitogenome sequence was represented between 1-11 × in the W chromosome. Phylogenetic analysis further revealed that these numts are an evolutionary recent acquisition of the W chromosome., Katrin Lämmermann, Heiko Vogel, Walther Traut., and Obsahuje bibliografii