Chronic allograft nephropathy (CAN) represents a frequent and irreversible cause of long-term renal graft loss. TGF- β 1 is a key profibrogenic cytokine associated with CAN pathogenesis. Because of clinical diagnostic inaccuracy, protocol biopsy has been suggested to be a beneficial method for early CAN detection. Protocol core biopsy was carried out in 67 consecutive cyclosporine-based immunosuppression-treated kidney transplant recipients with stable renal function 12 months after renal transplantation. Biopsy specimens were analyzed morphologically according to Banff-97' criteria and immunohistologically for TGF- β 1 staining. The data obtained were correlated with plasma TGF- β 1 levels and clinical data. CAN (grade I-III) was found in 51 patients (76 %). CAN grade I was found to be the most frequent one (44 %). A normal finding within the graft was made in only 12 patients (18 %). Clinically silent acute rejection Banff IA was present in 4 patients (6 %). In 8 patients (12 %) with CAN, borderline changes were present. We found a significant correlation between CAN grade and creatinine clearance, as measured by the Cockroft-Gault formula (p<0.01) as well as body mass index (p<0.01). There was a significant correlation between chronic vasculopathy (Banff cv) and creatinine clearance, and between the degree of TGF- β 1 staining and chronic vasculopathy (p<0.01). There were no relations between morphological findings and TGF- β 1 plasma levels, cyclosporine levels, plasma lipids, HLA- mismatches, panel reactive antibodies (PRA), proteinuria, and the donor's age. In conclusion, CAN is a frequent finding in protocol kidney graft biopsies 12 months after transplantation. TGF- β 1 tissue expression is linked with chronic vasculopathy., O. Viklický, I. Matl, L. Voska, R. Böhmová, M. Jarešová, J. Lácha, A. Lodererová, I. Stříž, V. Teplan, Š. Vítko., and Obsahuje bibliografii
The aim of this article is to sketch a certain method of indirect reconstruction of the process by which mathematics as a deductive discipline emerged in ancient Greece. We try out this method in a reconstruction of Thales' mathematics, but the main aim for which this method has been developed is the work of Pythagoras. We consider the process of the emergence of mathematics as a process of the constitution of a new language in the framework of which one can carry out deductive proofs. Therefore we base the method of indirect reconstruction of the emergence of mathematics on the theoretical findings in the book L. Kvasz: Vedecká revolúcia ako lingvistická událosť (The Scientific Revolution as a linguistic event, Prague, Filosofia 2013)., Ladislav Kvasz., and Obsahuje poznámky a bibliografii
Studie se zabývá částečným objevem hudebního rukopisu Thannenwald z polského přístavního města Elblag nedaleko Gdaňsku, který vznikl kolem roku 1600., Agnieszka Leszczyńska., Rubrika: Studie, and České resumé na s. 114, anglický abstrakt na s. 105
The aim of this study was to evaluate the influence of exercise with the intensity progressively increasing from rest until maximal oxygen uptake (VCbmax) on 2,3-DPG levels in red blood cells (RBC) in relation to the changes in the acid-base balance and plasma lactate concentration. Six healthy young men (age 22.5 ±1.5 years, V02max 3.48 ±0.20 1/min) participated in this study. The subjects performed an incremental exercise test on a cycloergometer until exhaustion. Blood samples were tested for acid-base balance indices (pH, HCO3-, BE), plasma lactate and RBC 2,3-DPG concentration. Gas exchange variables were measured continuously breath-bybreath. In this paper we present data concerning 2,3-DPG, plasma lactate, pH, HCO3" and BE measured at rest, at the power output corresponding to the lactate threshold (PO LT), at the power output at maximal oxygen uptake (PO VCbmax), as well as 5, 15 and 30 min after finishing the incremental test. Increase of power output above the lactate threshold to the PO V02max was accompanied by a significant (p<0.01) increase of plasma lactate from 2.58±0.78 mmol/1 to 10.22±3.04 mmol/1. This was also accompanied by a significant drop (p<0.01) in blood pH value from 7.352 ± 0.025 at the PO LT to 7.294 ±0.041 at the PO V02max- No significant changes of the RBC
2,3-DPG level were observed at any of the analysed stages of the exercise. The RBC 2,3-DPG level expressed in relation to the changes of haematocrit showed only minor changes during the exercise period and after 15 min of recovery vs. resting value (3.21 ±1.19). However, after 30 min of recovery, RBC 2,3-DPG decreased to the value of 2.32±1.19 /rmol/ml. We conclude that, during an incremental test, no increase in RBC 2,3-DPG concentration is required to reach the maximal oxygen uptake level. Moreover, a rapid decrease in blood pH, developing during a single bout of exercise, is not a stimulus powerful enough to cause significant changes in the RBC 2,3-DPG level during short-term exercise.