The general author‘s mesomechanical model with tensorial internal variables is applied to creep of steel 0,5Cr0,5Mo0,3V under different loading levels. It is shown that the creep process can be described by the mesomechanical model in its whole course up to rupture. However, the model parameters can be influenced at the very beginning by the application of the applied load if it is high enough to cause changes in the internal structure. This problem is specific for creep at high temperatures and significant differences in the loading levels (contrary to the small-strain plasticity studied usually at low temperatures, where the model parameters can be considered the same for different loadings). and Obsahuje seznam literatury
Steroid profiling helps various pathologies to be rapidly
diagnosed. Results from analyses investigating steroidogenic
pathways may be used as a tool for uncovering pathology
causations and proposals of new therapeutic approaches. The
purpose of this study was to address still underutilized application
of the advanced GC-MS/MS platform for the multicomponent
quantification of endogenous steroids. We developed and
validated a GC-MS/MS method for the quantification of
58 unconjugated steroids and 42 polar conjugates of steroids
(after hydrolysis) in human blood. The present method was
validated not only for blood of men and non-pregnant women
but also for blood of pregnant women and for mixed umbilical
cord blood. The spectrum of analytes includes common
hormones operating via nuclear receptors as well as other
bioactive substances like immunomodulatory and neuroactive
steroids. Our present results are comparable with those from our
previously published GC-MS method as well as the results of
others. The present method was extended for corticoids and
17α-hydroxylated 5α/β-reduced pregnanes, which are useful for
the investigation of alternative “backdoor” pathway. When
comparing the analytical characteristics of the present and
previous method, the first exhibit by far higher selectivity, and
generally higher sensitivity and better precision particularly for
17α-hydroxysteroids.
The paper introduces the test of aggregation as a simple, inexpensive method of evaluating suspension quality during drinking water treatment, suitable for use in both laboratory and operation conditions. The procedure and derivation of the aggregation test is described. The method is used for a demonstration of the influence of mean velocity gradient and mixing time on floc properties formed during the aggregation in a Couette reactor. It was proved that with increasing velocity gradient, the size of the aggregates present in the suspension decreases, and the suspension is substantially more homogeneous than with use of lower gradients. Further, it was confirmed that the size of aggregates reaches the steady state after a specific mixing time, which becomes shorter with increasing value of velocity gradient. and Příspěvek představuje test agregace jako jednoduchou a levnou metodu stanovení kvality suspenze při úpravě vody vhodnou pro použití v laboratorních i provozních podmínkách. Je popsán metodický postup i odvození testu agregace. Metoda je použita pro hodnocení vlivu středního gradientu rychlosti a doby míchání na vlastnosti vytvářených agregátů (vloček) při agregaci v Couettově reaktoru. Bylo prokázáno, že se vzrůstajícím gradientem se snižuje velikost agregátů přítomných v suspenzi a suspenze je značně více homogenní než při použití nízkých gradientů rychlosti. Dále bylo potvrzeno, že velikost agregátů dosahuje po určité době míchání stabilní hodnoty. Čas pro dosažení tohoto ustáleného stavu se zkracuje s rostoucí hodnotou použitého gradientu.
This paper describes an invented method for direct measurement of coalbed methane content in situ. In contrast to known procedures, this method does not need to place a rock or drilling cuts into an airtight canister, and does not involve sealing of the hole. Moreover, the new method is monitoring methane content in situ continuously and synchronously during drilling the hole not losing any portion of the gas. These positive features are a sequel of new approach based on injection of known portion of neutral gas into the hole. Methane content was determined from concentration of the mixture ‘methane-neutral gas´ at the hole´s mouth. New method is applicable for commercial recovery application of coalbed methane and forecast of dangerous gas and coal bursts. FLAC3D computer simulation helped to investigate dynamics of methane outflow from the hole to account for the effect of drilling speed on the rate of gas emanation.
A simple method of a bicolor (multicolor), fast-Fourier, PAM chlorophyll fluorometry has been developed to obtain fluorescence induction curves. Quantum yields of PSII photochemistry were determined with blue and red simultaneously applied pulsed measuring lights for three subsequent 20-min periods of dark-, light-adaptation under actinic light and dark recovery. Measuring lights were cross-combined with blue and red actinic lights and saturation pulses. Coefficients of chromatic divergence were calculated as a ratio of the quantum yields obtained by red measuring light to that obtained by blue measuring light. Adaptation of Ficus benjamina and Hordeum vulgare leaves under blue (but not red) actinic light resulted in the sufficient increase of chromatic divergence. In addition, fraction of active, non(photo)inhibited, PSII centers was shown to be dependent on the color of measuring light. Thus, color of the light sources should be considered when reporting results of parameters evaluated from fluorescence induction curves., V. Lysenko, D. Lazár, T. Varduny., and Obsahuje bibliografii
Text sentiment analysis plays an important role in social network information mining. It is also the theoretical foundation and basis of personalized recommendation, circle of interest classification and public opinion analysis. In view of the existing algorithms for feature extraction and weight calculation, we find that they fail to fully take into account the in fluence of sentiment words. Therefore, this paper proposed a fine-grained short text sentiment analysis method based on machine learning. To improve the calculation method of feature selection and weighting and proposed a more suitable sentiment analysis algorithm for features extraction named N-CHI and weight calculation named W-TF-IDF, increasing the proportion and weight of sentiment words in the feature words Through experimental analysis and comparison, the classification accuracy of this method is obviously improved compared with other methods.
This article reports a method for forecasting an earthquake by synchronous anomalies of optical astronomic time-latitude residuals. The so-called optical astronomic time-latitude residuals for a certain astrometric instrument are the rest after deducting the effects of Earth whole motion from the astronomical time and latitude observations determined by the instrument. Forecasting practice for four earthquakes around the Yunnan Observatory occurring after 2010 shows that it does not generate false forecasts, and also does not miss forecasts of major earthquakes. This forecasting practice proves that the synchronous anomalies of astronomical time-latitude residuals can provide effective warning sign for earthquake occurrence around observatory station, thus deserves attention and further study. and Su Youjin, Gao Yuping, Hu Hui.
When measuring cell membrane electrical capacitance in whole cell configuration using alternating currents, the resolution decreases with increasing membrane conductance and pipette resistance. Improved resolution was attained by the dual-frequency method which was modified as to control the voltage amplitude of one of the measuring frequencies. A model circuit was developed for the verification of the method. This circuit allows measurement of calibrated capacitance changes even in the range of 5 to 20 fF. Moreover, the method was applied to capacitance measurements on pancreatic exocrine acinar cells. The results of measurements on the model as well as on pancreatic acinar cells are presented. The principle can also be applied to other hardware and software methods for measuring electrical cell membrane parameters.
A system for the evaluation of temperature changes in living tissue at a dimensional level of a single cell is described. A glass micropipette the tip of which is filled with semiconducting glass (Rech et al. 1992), is used as a microsensor. The changes of conductivity of the sensor due to variations of temperature are evaluated by electronic circuitry based on the measurement of an AC current of sinusoidal waveform flowing through the sensor. Temperature changes in the range of 0.01 K can be detected in this way.
Transients of chlorophyll fluorescence in photosynthetic objects are often measured using short pulses of exciting radiation, which has recently been employed to capture kinetic images of fluorescence at the macroscopic level. Here we describe an instrument introducing this principle to recording of two dimensional fluorescence transients in microscopic objects. A modified fluorescence microscope is equipped with a CCD camera intensified by a micro-channel plate image amplifier. The microscopic field is irradiated simultaneously by three types of radiation: actinic radiation, saturating flashes, and pulsed measuring radiation. The measuring pulses are generated by a light-emitting diode and their duration is between 10 to 250 µs. The detection of fluorescence images (300×400 pixels, 8 bit) has a maximum time resolution of 40 ms and is gated in synchrony with the exciting pulses. This allows measuring on a background of a continuous actinic radiation up to irradiance that can elicit the maximal fluorescence yield (FM). On the other hand, the integral irradiance of the objects by the measuring radiation is very low, e.g., 0.08 µmol m-2 s-1 at 0.05 µm spatial resolution and 0.006 µmol m-2 s-1 at 4 µm spatial resolution. This allows a reliable recording of F0 even in very short time intervals (e.g., 5×80 ms). The software yields fluorescence kinetic curves for objects in user-selected areas as well as complete false-colour maps of the essential fluorescence kinetics parameters (FM, FO, FV, FV/FM, etc.) showing a two-dimensional distribution of their values. Several examples demonstrate that records of fluorescence kinetics can be obtained with a reasonable signal-to-noise ratio with all standard microscope objectives and with object sizes reaching from segments of leaf tissue to individual algal cells or chloroplasts. and H. Küpper ... [et al.].