Sledování lidských rodů je složitou součástí psychologického zkoumání. Projekt Vyhnání, vykořenění a změna životní linie - mezigenerační studie se zabývá psychologickými dopady aktu zvůle komunistické tajné policie v Československu, tzv. akce Asanace. Projekt navazuje na kumulující se zkušenost s transgeneračním traumatem. Zaměřuje se na generaci přímých účastníků, disidentů, kteří byli nuceni opustit svoji zem v letech 1977-1984, dále na potomky - druhou a třetí generaci, kteří v některých případech žijí v zahraničí. Pomocí čtyř druhů metod (fokusové skupiny, hloubkové rozhovory, dotazníkové metody, modifikovaný Stroopův test) se snaží porozumět transgenerační transmisi traumatu, zkoumá vedle traumatické situace také resilienci a posttraumatický růst. Zajímáme se o to, jak se traumatická situace transformuje v průběhu let, co přináší do pokračující rodové linie. Vedle standardních výzkumných článků plánují autoři také shrnující monografii na toto téma. and Tracking the family lineage is a complicated part of psychological research. The project titled Expulsion, uprooting, and change of lifeline-intergenerational study investigates the psychological impact of the communist secret police terror in Czechoslovakia (so-called Action “Asanace”), following the cumulation of research and therapeutic experience with transgenerational trauma. It focuses on the generation of the primary victims, dissidents forced to leave their homeland between 1977-84, as well as the 2nd and 3rd generation of their offspring, some of whom live abroad. It aims to understand the transition of trauma, resilience, and posttraumatic growth using several methods (focus groups, in-depth interviews, surveys, Modified Stroop Test). We shall examine transformations of the traumatic situation in time and its aftermath in the family lineage. Besides standard research papers, the authors plan a monograph.
The development of gestational diabetes mellitus (GDM) affects lipid metabolism during pregnancy. However, the magnitude of changes in lipid parameters is unclear. In addition, the patterns of these changes may vary based on the criteria selected for making the diagnosis of GDM. Thus, our aim was to compare the anthropometric and laboratory profiles of GDM-associated vs. GDM-free gestation with those of healthy non-pregnant women. We designed a cross-sectional study involving a group of females affected by GDM, a group of healthy pregnant controls and a group of healthy non-pregnant counterparts. GDM patients were divided into 3 subgroups according to the fulfilled diagnostic criteria, that is, those presenting with high fasting plasma glucose in the first trimester (subgroup 1), high fasting plasma glucose in the second trimester (subgroup 2) and high plasma glucose following oral glucose load in the second trimester (subgroup 3). The anthropometric and metabolic profiles of GDM subjects resembled the facets of metabolic syndrome (highest body mass index, waist circumference, C-peptide level, triglycerides) significantly more than the respective profiles of healthy non-pregnant women (p<0.0001). While total cholesterol (TC) (together with LDL-C and non-HDL-C) in pregnant women with GDM and without GDM did not differ, both groups had significantly higher levels of triglycerides (TG) than non-pregnant women (p<0.0001). Subgroup 1 had the highest fasting glucose level in the second trimester whereas subgroup 3 had the lowest fasting glucose level (p=0.019). Concentration of TG increased, being the lowest in subgroup 1 and the highest in subgroup 3 (p=0.006). Women with GDM had more pronounced features of metabolic syndrome than pregnant women without GDM. Both groups reached higher levels of TC (LDL-C, non-HDL-C) than non-pregnant controls and did not differ from each other. We found differences in TG and fasting glucose levels among different types of GDM
Cílem této studie je představit překlad a adaptaci Škály pracovní dedikace (Job Dedication Scale) do češtiny. Výzkumný soubor tvořilo 142 pracovníků v sociálních službách domovů pro seniory napříč Českou republikou, kteří byli hodnoceni svými přímými nadřízenými Škálou pracovní dedikace. Překlad proběhl formou zpětných translací s využitím tří na sobě nezávislých překladatelů. Popisná statistika přinesla zjištění o negativní šikmosti udělených hodnocení a snížené variabilitě odpovědí u některých položek. Konfirmační faktorová analýza plné verze škály nepotvrdila i přes vysokou hodnotu vnitřní konzistence shodu modelu se získanými daty. V návaznosti na výsledky faktorových analýz a modifikačních indexů byly povoleny reziduální korelace mezi vybranými položkami, což zvýšilo kvalitu testovaného modelu. Výpočty vnitřní konzistence ukázaly excelentní hodnoty, přičemž vyřazení jakékoliv položky by tuto hodnotu snížilo. Limity studie spatřujeme v malém a specifickém výzkumném souboru, a také v přístupu k posuzování pracovní dedikace zaměstnance s využitím pouze jednoho hodnotitele. and This study aims to present the translation and adaptation of the Job Dedication Scale into Czech. The final sample consisted of 142 workers in the social services of retirement homes across the Czech Republic, evaluated by their direct superiors on the Job Dedication Scale. The translation took the form of back translations using three independent translators. Descriptive statistics showed the negative skewness of the given ratings and the reduced variability of responses for some items. Despite the high value of internal consistency, the confirmatory factor analysis of the full version of the scale did not demonstrate agreement with the obtained data. Following the results of factor analyses and modification indices, residual correlations between selected items were allowed, which increased the quality of the tested model. The modified model showed a good match with the data. Values of the internal consistency of the scale were excellent. Eliminating any item would reduce this value. The small and specific sample is one of the limits of the study. Another limit is an approach to assessing the job dedication of the employee using only one evaluator.
Intracellular bacteria of the genus Wolbachia (α-Proteobacteria) are the most widespread endosymbionts of insects. Host infection is usually associated with alterations in reproduction, such as cytoplasmic incompatibility, the induction of parthenogenesis and offspring sex ratio bias: all phenomena that may influence host speciation. In the present study, by using well-established molecular tools, we investigated the presence of Wolbachia in leaf beetles of the genus Crioceris and their host plants, which are various species of Asparagus. Multilocus sequence typing of bacterial genes showed that despite their occurrence in the same habitat and feeding on the same plant, two species of Crioceris, C. quinquepunctata and C. quatuordecimpunctata, are infected by two different strains of Wolbachia. C. asparagi, C. paracenthesis and C. duodecimpunctata, which are sympatric with the infected species, do not harbour the bacterium. Interestingly, DNA of Wolbachia was detected in host plant tissues that are exploited by the beetles, providing evidence for the horizontal transmission of the bacterium between beetles and their host plants. Moreover, Wolbachia was detected in species of Crioceris that are not closely related., Michał Kolasa, Matteo Montagna, Valeria Mereghetti, Daniel Kubisz, Miłosz A. Mazur, Łukasz Kajtoch., and Obsahuje bibliografii
In the present study, we investigated the effect of acrylamide (ACR) exposure during pregnancy on the ovary of female adult offspring of two subsequent generations. Sixty-day-old Wistar albino female rats were given different doses of ACR (2.5 and 10 mg/kg/day) from day 6 of pregnancy until giving birth. Females from the first generation (AF1) were fed ad libitum, and thereafter, a subgroup was euthanized at 8 weeks of age and ovary samples were obtained. The remaining females were maintained until they reached sexual maturity (50 days old) and then treated in the same way as the previous generation to obtain the second generation of females (AF2). The histopathological examination indicated a high frequency of corpora lutea along with an increased number of antral follicles that reached the selectable stage mainly at a dose of 2.5 mg/kg/day. Interestingly, ACR exposure significantly increased the mRNA levels of CYP19 gene and its corresponding CYP19 protein expression in AF1 females. The TUNEL assay showed a significantly high rate of apoptosis in stromal cells except for dose of 2.5 mg/kg/day. However, in AF2 females, ACR exposure significantly increased the number of degenerating follicles and cysts while the number of growing follicles was reduced. Moreover, in both ACR-treated groups, estradiolproducing enzyme CYP19A gene and its corresponding protein were significantly reduced, and an excessive apoptosis was produced. We concluded that the ovarian condition of AF1 females had considerable similarity to the typical early perimenopausal stage, whereas that of AF2 females was similar to the late perimenopausal stage in women.
Babesia caballi (Nuttal, 1910) is one of the causative agents of equine piroplasmosis which causes economic losses to horse industry in China. There is an urgent need for rapid detection method for B. caballi infection in Xinjiang Province, China. To prepare monoclonal antibodies (mAbs) against Bc48 gene of B. caballi (Xinjiang local strains) and establish colloidal gold-immunochromatographic (ICT) assay for diagnosis of the disease, recombinant Bc48 was expressed and purified from Escherichia coli. With purified Bc48 as immunogen in mice, three hybridoma cells named 11F4, 1H2 and 7F4 secreting mAbs against Bc48 of B. caballi were obtained, which showed strong reaction with recombinant Bc48 and Bc48 gene transfected cells. Furthermore, colloidal gold labelled ICT assay based on purified Bc48 recombinant antigen and its mAb was developed. The ICT assay showed high sensitivity and specificity and no cross-reaction with Theileria equi (Laveran, 1901). Total of 56 horse serum samples collected from Xinjiang were tested by ICT and compared with the detection by commercial ELISA kit. The results showed that 32 out of 56 serum samples were positive by ICT and 33 were positive by ELISA. ICT assay had high coincidence (98%) to the reference ELISA kit. mAbs and ICT developed in this study could be provided as an efficient diagnosis tool for infection with B. caballi in horse in Xinjiang area., Panju Wang, Jingjing Song, Ruiqi Song, Mengyuan Zhang, Lijiang Wu, Fangxin Li, Yan Yan, Jiyong Zhou, Bayin Chahan, Min Liao., and Obsahuje bibliografii
Some free-living amoebae are a potential threat to human health. The best known species are those of the genus Acanthamoeba Volkonsky, 1931, which cause Acanthamoeba keratitis, granulomatous amoebic encephalitis and other forms of tissue inflammation. The aim of the present study was to search for potential pathogenic genotypes of free-living amoeba in the sand in children's playgrounds. Our results confirmed that free-living amoebae were present in all examined playgrounds. Sequences of the 18S rDNA have shown that all isolated potentially pathogenic strains of amoebae belong to genotype T4 of Acanthamoeba. The potential pathogenicity of isolates was confirmed on mice. The presence of pathogenic amoebae in the examined sand may be a potential source of human infection., Marcin Cholewiński, Piotr Solarczyk, Monika Derda, Agnieszka Wojtkowiak-Giera, Edward Hadaś., and Obsahuje bibliografii
The gut microbiota provides a wide range of beneficial functions for the host, and has an immense effect on the host’s health status. The presence of microbiome in the gut may often influence the effect of an orally administered drug. Molecular mechanisms of this process are however mostly unclear. We investigated how the effect of a nonsteroidal drug nabumetone on expression of drug metabolizing enzymes (DMEs) in mice intestine and liver is changed by the presence of microbiota, here, using the germ free (GF) and specific pathogen free (SPF) BALB/c mice. First, we have found in a preliminary experiment that in the GF mice there is a tendency to increase bioavailability of the active form of nabumetone, which we have found now to be possibly influenced by differences in expression of DMEs in the GF and SPF mice. Indeed, we have observed that the expression of the most of selected cytochromes P450 (CYPs) was significantly changed in the small intestine of GF mice compared to the SPF ones. Moreover, orally administered nabumetone itself altered the expression of some CYPs and above all, in different ways in the GF and SPF mice. In the GF mice, the expression of the DMEs (CYP1A) responsible for the formation of active form of the drug are significantly increased in the small intestine and liver after nabumetone application. These results highlight the importance of gut microbiome in processes involved in drug metabolism in the both gastrointestinal tract and in the liver with possible clinical relevance.