The history of Olomouc book printing remains mostly unexplored. Literature often brings distorted information, which must first be verified by studying sources. An example may be the Olomouc printer Vít Jindřich Ettel (+ 1669), about whom some researchers have accumulated a large amount of unverified or misleading information. Nevertheless, numerous archival sources from which it is possible to reconstruct the life and activities of this book printer are available as well. Attention should, however, also be paid to his wife, Anna Alžběta Ettelová, who, after her husband’s death, administered the local printing house alone for three years (until January 1673). Multiple archival sources make it possible to study her activities in further detail and to learn more not only abo, Miroslav Myšák., Obsahuje anglický abstrakt a shrnutí., and Obsahuje bibliografii
Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNFα), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFα 5-fold, p<0.05; MCP-1 3.6-fold, p<0.05; IL-6 18.8-fold, p<0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFα concentration in patients (4.86± 1.4 pg/ml) compared to LKDs (2.14±0.9 pg/ml; p<0.001), as was hsCRP (11.8±7.0 in PAD; 1.5±0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFα and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis., S. Čejková, I. Králová Lesná, J. Froněk, L. Janoušek, A. Králová, J. Ždychová, R. Poledne., and Obsahuje bibliografii
Cosmic reionization, which took place in the first billion years after the Big Bang, was a major event in the cosmic history. This transformation of all neutral hydrogen to ionize was probably caused by the first stars, galaxies and quasars. However, a direct proof of which sources were dominant is still missing. Dr. Ivana Orlitová from the Institute of Astronomy of the CAS reviews results of the serach for ionizing sources in both distant and nearby Universe, and presents a recent discovery of starforming galaxies that show signs of strong ionizing activity. and Ivana Orlitová.
Nebezpečným, ale mezi studenty oblíbeným pokusem demonstrujícím explozivní chemickou reakci je vhodit kousek kovového sodíku do vody. Každý učitel chemie ví, že výbuch nastává kvůli uvolňování tepla při přechodu elektronů z kovu do vody, přičemž vzniká pára, hydroxid a vodík, který se může vznítit. Plyny vznikající na rozhraní mezi kovem a vodou by ale měly od sebe tyto reaktanty oddělit a tím reakci potlačit. Jak to, že k výbuchu přesto dochází? Pomocí záběrů ultrarychlé kamery a molekulových simulací se podařilo odhalit dosud neznámý primární mechanismus explozivní reakce alkalických kovů ve vodě. Poté, co elektrony přejdou z alkalického kovu do vody, vytvoří se na jeho povrchu značně veliký kladný náboj. Kvůli vzájemnému odpuzování těchto nábojů dojde k takzvané coulombické explozi, kdy s povrchu ohromnou rychlostí vylétají kousky kovu do vody. Tím se reaktanty efektivně promíchají, což je podmínkou výbuchu., Throwing a piece of sodium into water is a dangerous, but popular experiment demonstrating an explosive chemical reaction. Every chemistry teacher knows that during the explosion heat is released when electrons move from the metal to water generating water vapor, hydroxide, and hydrogen which can ignite. Gases generated at the interface between the metal and water should, however, separate the reactants and thus quench the explosion. How is it possible that the explosion can nevertheless take place? Thanks to pictures from an ultrafast camera and molecular simulations researchers from the Institute of Organic Chemistry and Biochemistry found a hitherto unknown primary mechanism of the explosive reaction of alkali metals in water. When the electrons move from the metal to water, the former acquires a large positive charge. Repulsion between these charges leads to a Coulomb explosion with metal spikes shooting into water. This leads to effective mixing of reactants thus enabling the explosion., and Pavel Jungwirth.
This new project of the Institute of Physics of the CAS is a Widening Excellence, Twinning project aimed at boosting the scientific excellence and technology-transfer capacity in advanced scintillating materials of the Institute of Physics from the Czech Academy of Sciences by creating a network with four other partners: CERN, Institute Lumière Matière - Université Claude Bernard Lyon 1, Università degli Studi di Milano - Biocca and Intelligentsia Consultants. and Jana Olivová.
Recently, we have established a model of severe stepwise normovolemic hemodilution to a hematocrit of 10 % in rats employing three different colloidal volume replacement solutions (Voluven, Volulyte and Gelafundin) that are routinely used in clinical practice at present. We did not see severe dilutional acidosis as to be expected, but a decline in urinary pH. We here looked on further mechanisms of renal acid excretion during normovolemic hemodilution. Bicarbonate, which had been removed during normovolemic hemodilution, was calculated with the help of the Henderson-Hasselbalch equation. The urinary amount of ammonium as well as phosphate was determined in residual probes. The absolute amount of free protons in urine was obtained from the pH of the respective samples. The amount of protons generated during normovolemic hemodilution was approximately 0.6 mmol. During experimental time (5.5 h), distinct urinary ammonium excretion occurred (Voluven 0.52 mmol, Volulyte 0.39 mmol and Gelafundin 0.77 mmol). Proton excretion via the phosphate buffer constituted 0.04 mmol in every experimental group. Excretion of free protons was in the range of 10-6 mmol. The present data prove that the prompt rise in urinary ammonium excretion is also valid for acute metabolic acidosis originating from severe normovolemic hemodilution., J. K. Teloh, I. N. Waack, H. de Groot., and Obsahuje bibliografii
Proteinuria is often used as a surrogate marker in monitoring and predicting outcome in patients with chronic kidney diseases, but it is non-specific. IgAN belongs to the most common primary glomerulonephritis worldwide with serious prognosis. The main aim of this work was to assess differences in urine proteins in patients with IgA nephropathy and to identify abnormal proteins as potential biomarkers of IgA nephropathy or the renal disease. In our pilot project, we selected 20 patients and compared them with 20 healthy volunteers. Protein quantification was performed using iTRAQ (isobaric tag for relative and absolute quantitation) labeling method. The peptides were separated by the isoelectric focusing method (IEF) and nano-LC with C18 column and identified by mass spectrometry using MALDI-TOF/TOF MS. Proteins´ lists obtained from IEF-LC-MS-MS/MS analysis were combined and contained 201 proteins. It was found out that 113 proteins were common in both experiments. 30 urinary proteins were significantly up- or down-regulated in patients with IgA nephropathy. We characterized potential biomarkers such as alpha-1-antitrypsin, apolipoprotein A-I, CD44 antigen or kininogen. Potential biomarkers of IgAN should be validated in further studies., P. Prikryl, L. Vojtova, D. Maixnerova, M. Vokurka, M. Neprasova, T. Zima, V. Tesar., and Obsahuje bibliografii