Species of Blastocystis Alexieff, 1911 are anaerobic intestinal protists found in humans and many kinds of animals that mainly cause diarrhea, abdominal pain and other clinical symptoms. At present, data on the prevalence and subtype diversity of species of Blastocystis in domestic rabbits are very limited. The purpose of this study was to characterise the infection rate and gene subtype distribution of Blastocystis sp. in domestic rabbits in Henan Province, Central China, and provide foundation for prevention and control of the disease caused by Blastocystis sp. in domestic rabbits. DNA was extracted from 286 fresh rabbit faecal samples collected from four areas of Henan Province, Central China. All DNA samples were screened using PCR and positive samples were sequenced to identify individual subtypes based on the small ribosomal subunit (SSU rRNA) gene. The overall infection rate of Blastocystis sp. in domestic rabbits in Henan Province was 15% (43/286). Three subtypes were identified, including ST1 (26/43, 60%), ST3 (5/43, 12%) and ST7 (12/43, 28%), all of which belonged to potentially zoonotic subtypes, ST1 was the dominant gene subtype. These results showed that infection with Blastocystis sp. was common in domestic rabbits in Henan Province, Central China, and was represented by zoonotic subtypes. Therefore, special attention should be paid to reduce the risk of transmission of Blastocystis sp. from domestic rabbits to humans.
The care of patients with severe mental illness (SMI) is often narrowed down to the treatment of biological symptoms of the disease, whilst other psychosocial factors supporting mental health are often neglected. One of these factors influencing disease progression, compliance, self-concept, and motivation for treatment is parenthood. The first step to turning attention to this issue is to be aware of the prevalence of children living with a parent with SMI and the prevalence of parenting among SMI patients. This review aims to assess the current state of knowledge regarding methodological approaches, the prevalence rates of parenthood, the number of children living with a parent with SMI, and the sociodemographic characteristics of these families. A total of 1,156 records were retrieved from EBSCO Discovery in December 2021, and fifteen published studies addressed the review questions. The studies took various approaches to measure prevalence - an epidemiological approach, a census approach, a design in which data from national registries were analysed, or cross-sectional studies in a clinical population. Nine studies investigated the prevalence of parenthood among patients with SMI, and six studies quantified the number of children living with a parent with mental illness. 1/3 of mental health service clients were parents, and 1 to 2 children out of 10 have a parent with mental illness.Parents with SMI face lower education, lower employment, lower income, higher rates of comorbid substance abuse, and higher rates of single parenthood. The aim of the review is not to give a definitive answer to the question of prevalence as much as to point out that there is not a marginal number of children living with a parent with a psychiatric disorder. and Péče o pacienty se závažným duševním onemocněním (SMI) se často zužuje na léčbu biologických příznaků nemoci, zatímco ostatní psychosociální faktory podporující duševní zdraví jsou často opomíjeny. Jedním z těchto faktorů ovlivňujících průběh léčby, komplianci, sebepojetí pacienta a motivaci k léčbě, je rodičovství. Prvním krokem k obrácení pozornosti k této problematice je uvědomění si míry prevalence dětí žijících s rodičem se SMI a míry prevalence rodičovství mezi pacienty se SMI. Cílem tohoto přehledu je zhodnotit metodologické přístupy využívané k měření prevalence, míru prevalence rodičovství, počet dětí žijících s rodičem se SMI a sociodemografické charakteristiky těchto rodin. V databázi EBSCO Discovery bylo v prosinci 2021 vyhledáno celkem 1 156 záznamů, výběrová kritéria splnilo patnáct publikovaných studií, které byly zařazeny do přehledu. Studie využívaly různé přístupy k měření prevalence - epidemiologický přístup, přístup založený na cenzu, design, v němž byla analyzována data z národních registrů, nebo průřezové studie v klinické populaci. Devět studií zkoumalo prevalenci rodičovství u pacientů s duševním onemocněním a šest studií kvantifikovalo počet dětí žijících s rodičem s duševním onemocněním. Rodiče tvořili 1/3 klientů psychiatrických zařízení a 1 až 2 děti z 10 mají rodiče s duševním onemocněním. Rodiče se SMI se potýkají s nižším vzděláním, nižší zaměstnaností, nižšími příjmy, vyšší mírou komorbidního užívání návykových látek a vyšší mírou samoživitelství. Cílem přehledu není dát definitivní odpověď na otázku prevalence, ale spíše poukázat na to, že dětí žijících s rodičem s psychiatrickou poruchou není zanedbatelné množství.
The principle of double effect plays an important role in deontological ethics which give an emphasis to intentions in the process of moral evaluation of human acts. According to this principle, it is morally permissible to actualize an action with two consequences, one of which is good and the second bad, if and only if some conditions are met: for example, the bad consequence is not a means for attaining the good consequence and the bad consequence is not brought about intentionally, but only foreseen as necessarily and inevitably connected with the actualization of the action itself and its good consequence. The aim of this article is to lay necessary theoretical foundations for a better understanding and employment of the principle of double effect by discussing Hart’s critique of it and Philippa Foot’s different approach to the moral dilemmas to the solution of which the principle of double effects seems to be well applicable. In the course of my argumentation I distinguish between two types of intentions – characterizing and final intentions – and show how this distinction solve some of the difficulties connected with the understanding and application of the principle of double effect. and Princip dvojího účinku hraje důležitou roli v deontologických etikách, které přikládají důležitost intencím v procesu morálního hodnocení lidského jednání. Podle tohoto principu je morálně přijatelné aktualizovat jednání se dvěma důsledky, z nichž první je dobrý a druhý špatný, pouze za předpokladu, že jsou splněny určité podmínky: špatný důsledek například nesmí být prostředkem k dosažení dobrého důsledku a špatný důsledek nesmí být způsobený úmyslně, je pouze předvídaný jako nutně a nevyhnutelně spojený s provedením jednání samotného a s jeho dobrým důsledkem. Cílem tohoto článku je vystavět nezbytné teoretické předpoklady potřebné k lepšímu pochopení a aplikaci principu dvojího účinku. Diskutuji kritiku tohoto principu britským teoretikem práva Hartem a odlišný přístup k řešení morálních dilemat, k jejichž řešení se princip dvojího účinku zdá vhodným, v díle Philippy Footové. V průběhu své argumentace rozlišuji mezi dvěma typy intencí – charakterizující intence a finální intence – a ukazuji, jak tento rozdíl pomáhá řešit některé obtíže spojení s chápáním a aplikací principu dvojího účinku.
Eva Fialová, Ján Matejka, Vojen Güttler., Právní stav publikace je k 31. 12. 2019., Seznam zkratek, Obsahuje bibliografii a bibliografické odkazy, and born digital
Progesterone is a steroidal hormone that is produced from the corpus luteum of the ovaries and from the placenta. The main function of progesterone is to promote the secretory differentiation in the endometrium of the uterus and to maintain pregnancy by inhibiting uterine contractions throughout pregnancy. Progesterone performs its actions by activating the classical progesterone nuclear receptors that affect gene transcription and by the non-classical activation of cell surface membrane receptors that accounts for the rapid actions of progesterone. Besides the reproductive roles of progesterone, it exerts functions in many tissues and systems such as the nervous system, the bone, the vascular system, and the gastrointestinal (GI) tract. This review will summarize the recent literature that investigated the role of progesterone in GI tract motility. Most literature indicates that progesterone exerts an inhibitory role on gut smooth muscle cells in part by elevating nitric oxide synthesis, which induces relaxation in smooth muscle. Moreover, progesterone inhibits the signaling pathways that lead to contraction such as Rho kinase inhibition. These data serve as a quick resource for the future directions of progesterone research that could lead to better understanding and more effective treatment of gender-related GI tract motility disorders.
Below-average precipitation and above-average air temperature are important factors in the occurrence and intensity of drought. In the context of global climate change, air temperature increase, as a key climatological parameter, has to be considered when calculating the drought index. We introduce a new method of drought analysis, relying on standardized values of precipitation and mean air temperatures for a certain period. The standardized value is calculated by subtracting the average value for each period from each measured value and dividing the obtained value by the standard deviation of the sample. Next, the New Drought Index (NDI) is calculated by subtracting the standardized temperature value from the standardized precipitation value. NDI values were determined for the monthly and annual precipitation time series and mean monthly and annual air temperatures measured at the stations Split-Marjan and Zagreb-Grič between 1948 and 2020. The NDI indicates that the risk of drought has intensified significantly in recent decades, which may be related to the effect of global warming.
Acute lung injury (ALI) caused by lipopolysaccharide (LPS) is a common, severe clinical syndrome. Injury caused by inflammation and oxidative stress in vascular endothelial and alveolar epithelial cells is a vital process in the pathogenesis of ALI. Toll-like receptor 9 (TLR9) is highly expressed in LPS-induced ALI rats. In this study, Beas-2B human pulmonary epithelial cells and A549 alveolar epithelial cells were stimulated by LPS, resulting in the upregulation of TLR9 in a concentrationdependent manner. Furthermore, TLR9 overexpression and interference vectors were transfected before LPS administration to explore the role of TLR9 in LPS-induced ALI in vitro. The findings revealed that inhibition of TLR9 reduced inflammation and oxidative stress while suppressing apoptosis of LPS-induced Beas-2B and A549 cells, whereas TLR9 overexpression aggravated these conditions. Moreover, TLR9 inhibition resulted in downregulated protein expression of myeloid differentiation protein 88 (MyD88) and activator activator protein 1 (AP-1), as well as phosphorylation of nuclear factor-κB (NF-κB), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK). The phosphorylation of extracellular-regulated protein kinases 1/2 was upregulated compared to that of cells subjected to only LPS administration, and this was reversed by TLR9 overexpression. These results indicate that inhibition of TLR9 plays a protective role against LPS-induced inflammation and oxidative stress in Beas-2B and A549 cells, possibly via the MyD88/NF-κB and MyD88/MAPKs/AP-1 pathways.