Buffaloes represent an important economic resource for several regions of the world including Romania. In the present study, we examined 104 faecal samples collected from 38 buffalo calves (2-11 weeks old) from household rearing systems in Romania for gastrointestinal parasites. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with sequencing isolates were used to identify assemblages of Giardia lamblia (Kunstler, 1882) and species of Cryptosporidium Tyzzer, 1907. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites: 16 had single infections and 17 had mixed infections with two or three parasites. Species of Eimeria Schneider, 1875 (32/38; 84%) were the most prevalent parasites; eight species were identified according to the oocyst morphology, including the pathogenic E. bareillyi (Gill, Chhabra et Lall, 1963) which was detected for the first time in buffaloes from Romania. The nematodes Toxocara vitulorum (Goeze, 1782) (11/38; 37%) and Strongyloides papillosus (Wedl, 1856) (6/38; 16%) were also detected. Cryptosporidium spp. were found in four (11%) buffalo calves; two of them were molecularly identified as C. ryanae Fayer, Santin et Trout, 2008, and another one clustered in the same clade with C. ryanae, C. bovis Fayer, Santin et Xiao, 2005, and C. xiaoi Fayer et Santin, 2009. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.
This study tested whether cell cycle inhibitor mitomycin C (MMC) prevents arthrogenic contracture progression during remobilization by inhibiting fibroblast proliferation and fibrosis in the joint capsule. Rat knees were immobilized in a flexed position to generate flexion contracture. After three weeks, the fixation device was removed and rat knees were allowed to freely move for one week. Immediately after and three days after fixator removal, rats received intra-articular injections of MMC or saline. The passive extension range of motion (ROM) was measured before and after myotomy of the knee flexors to distinguish myogenic and arthrogenic contractures. In addition, both cellularity and fibrosis in the posterior joint capsule were assessed histologically. Joint immobilization significantly decreased ROMs both before and after myotomy compared with untreated controls. In saline-injected knees, remobilization increased ROM before myotomy, but further decreased that after myotomy compared with that of knees immediately after three weeks of immobilization. Histological analysis revealed that hypercellularity, mainly due to fibroblast proliferation, and fibrosis characterized by increases in collagen density and joint capsule thickness occurred after remobilization in saline-injected knees. Conversely, MMC injections were able to prevent the remobilization-enhanced reduction of ROM after myotomy by inhibiting both hypercellularity and joint capsule fibrosis. Our results suggest that joint capsule fibrosis accompanied by fibroblast proliferation is a potential cause of arthrogenic contracture progression during remobilization, and that inhibiting fibroblast proliferation may constitute an effective remedy.
The taxonomy of myxosporeans was traditionally dependent solely upon the spore morphological and morphometric data. Intensive reports of intraspecific morphological variation, however, are increasingly challenging the taxonomic approaches for myxosporeans. In the present work, the morphological pleomorphism of myxospores of Myxobolus drjagini (Akhmerov, 1954) was observed. More interestingly, all of these pleomorphic myxospores occurred in the same plasmodium of M. drjagini, which refutes the previous hypothesis that morphological variation of M. drjagini was derived from its responses to differences in nutrition and immunological responses associated with different host tissues. Bearing the intraspecific morphometric and morphotype variation in mind, the combination of morphological, ecological and molecular data should be applied to the species identification and delimitation for myxosporeans. This is the first reported myxobolid species with high pleomorphic myxospores which are present in the same plasmodium.
Morphometric data from spores of ten myxosporean species were statistically analysed to explore myxosporean intraspecific variation in measurements when obtained from a sample from: (1) the same plasmodium, (2) different plasmodia from the same host and (3) different host individuals and localities. In some cases, significant differences in spore dimensions were found between samples from the same plasmodium, highlighting the difficulty of obtaining representative measurements of myxosporean spore. In addition, significant differences in spore dimensions were found when plasmodia from the same site of infection were compared, suggesting that measurements of spores should come from several different plasmodia of the sampling to increase the reliability of the morphology data. Moreover, significant differences in spore dimensions were observed for most spore dimensions when data were compared between localities. In all cases, there was clear overlap in ranges of dimensions even when means differed significantly. The present statistical analysis shows that intraspecific morphometric variation of myxosporean species commonly occurs, highlighting the importance of reporting ranges of measurements for a species, not just the mean dimensions, and taking into account all evidence when assigning or describing myxosporean species., Yanhua Zhai, Christopher M. Whipps, Zemao Gu, Qingxiang Guo, Zizhen Wu, Hongmei Wang, Yang Liu., and Obsahuje bibliografii
Cancer is a complex, multifactorial disease that modern medicine ultimately aims to overcome. Downstream of tyrosine kinase 2 (DOK2) is a well-known tumor suppressor gene, and a member of the downstream protein DOK family of tyrosine kinases. Through a search of original literature indexed in PubMed and other databases, the present review aims to extricate the mechanisms by which DOK2 acts on cancer, thereby identifying more reliable and effective therapeutic targets to promote enhanced methods of cancer prevention and treatment. The review focuses on the role of DOK2 in multiple tumor types in the lungs, intestines, liver, and breast. Additionally, we discuss the potential mechanisms of action of DOK2 and the downstream consequences via the Ras/MPAK/ERK or PI3K/AKT/mTOR signaling pathways., Pei Sun, Rumeng Li, Yingying Meng, Shijun Xi, Qinqi Wang, Xiulan Yang, Xiaochun Peng, Jun Cai., and Obsahuje bibliografii
Pulmonary pathology is common in HIV-infected individuals, but the possible role of the parasitic protist Toxoplasma gondii (Nicolle et Manceaux, 1908) is not completely known. The present account reports result of a retrospective cohort study. Medical cards of 907 HIV-positive people, which included 120 deceased patients, were analysed. During a three-year follow-up, the pulmonary pathology was diagnosed in 306 patients (33.7 ± 1.6%): pneumocystis pneumonia in 124 (13.7 ± 1.1%), primary pulmonary tuberculosis in 113 (12.5 ± 1.1%), bacterial pneumonia in 58 (6.4 ± 0.8%) toxoplasmosis pneumonia in two (0.2 ± 0.2%), and others. All patients were divided into two cohorts: 531 individuals seropositive for T. gondii and 376 seronegative ones. It has been found out that general lung pathology is more common in patients with seropositivity to T. gondii than in seronegative ones (43.3 ± 2.2% vs. 20.1 ± 2.0%, p < 0.001). The diagnosis of pneumocystis pneumonia was made ten times more often in the cohort of seropositive patients than in the cohort of seronegative ones (21.9 ± 1.8% vs. 2.1 ± 0.7%, respectively, p < 0.001) and in deceased patients of these cohorts it was 5.5 times more (45.1 ± 5.9% vs. 8.2 ± 3.9, respectively, p < 0.001). In patients with fatal outcome and seropositivity to T. gondii, the incidences of pneumocystis pneumonia increased by 23.2% (p < 0.001) and bacterial pneumonia by 12.4% (p < 0.05), whereas in seronegative individuals only pulmonary tuberculosis increased by 13.1% (p < 0.05) сompared with corresponding whole cohorts. Pearson's contingency coefficient showed the mean strength association between infection with T. gondii and incidence of pneumocystis pneumonia both in whole cohort (C = 0.272) and in patients with fatal outcomes (C = 0.368). In сonclusion, significantly increasing rate of pneumocystis pneumonia in patients with HIV/AIDS and T. gondii infection can be caused by certain synergism between T. gondii and Pneumocystis jirovecii and in some cases overdiagnosis pneumocystis pneumonia due to undiagnosed toxoplasmosis pneumonia.
Tato studie je pilotáží nové metody pro měření náchylnosti k prožívání viny a hanby, Inventáře tendence k vině a hanbě (ITVAH). ITVAH je metoda založená na použití scénářů, která rozlišuje afektivní a behaviorální složky těchto dispozic a zároveň zohledňuje soukromé, resp. veřejné prožívání viny a hanby. Pro každý z 16 scénářů byly vytvořeny dvě položky – afektivní a behaviorální reakce, čímž vzniklo celkem 32 položek. Byl předpokládán čtyřfaktorový model měření – Negativní hodnocení chování (NHCh), Náprava, Negativní hodnocení self (NHS) a Únik. Dále byla předpokládána negativní souvislost NHS a Úniku se sebehodnocením a tendencí k sebeodpuštění a pozitivní souvislost NHCh a Nápravy s tendencí k sebeodpuštění. Pilotní studie proběhla na vzorku 249 česky mluvících dospělých (67 % žen, věk 18–75, Md = 22). Na základě ordinální konfirmační faktorové analýzy se zohledněním reziduálních kovariancí mezi položkami vztahujících se ke stejnému scénáři jsme zjistili akceptovatelnou shodu předpokládaného modelu s daty. Pro předpokládané souvislosti se sebehodnocením a tendencí k sebeodpuštění jsme však získali pouze částečnou oporu, v rozporu s teoretickým očekáváním jsme zjistili středně silný negativní vztah mezi tendencí k sebeodpuštění a afektivní složkou viny. Pro širší používání této metody v československém prostředí je důležité dále ověřit konstruktovou validitu metody společně s ověřením souběžné validity s The Test Of Self-Conscious Affect-3 (TOSCA-3). and This paper constitutes a pilot study of a novel measure for assessing proneness to guilt and proneness to shame, Inventář tendence k vině a hanbě (ITVAH, Inventory of Proneness to Guilt and Shame). ITVAH is a scenario-based method distinguishing affective and behavioural components of guilt and shame and also considering the private or public experience of guilt and shame, respectively. Two items (affective and behavioural reaction) were created for each of the 16 scenarios, resulting in total of 32 items. A four-factor measurement model was assumed – Negative behaviour evaluation (NBE), Reparation, Negative self-evaluation (NSE), Withdrawal. Next, we assumed negative correlations of NSE and Withdrawal with self-esteem and self-forgiveness and positive correlations of NBE and Reparation with self-forgiveness. The pilot study was carried out on 249 Czech-speaking adults (67 % women, aged 18–75, Md = 22). An ordinal confirmatory factor analysis with residual covariances between items connected to the same scenarios was conducted. The hypothesized four-factor model was found acceptable. However, we found limited evidence for the hypothesized relationships with self-esteem and self-forgiveness – in contrast to theoretical expectations we found a medium-strong negative relationship between self-forgiveness and affective component of guilt. For further use of the measure in the Czech and Slovak environment it is necessary to assess additional evidence of construct validity and concurrent validity with The Test of Self-Conscious Affect-3 (TOSCA-3).
The use of livestock guarding dogs (LGDs) has been widely advocated as a responsible tool for reducing livestock predation and conserving wildlife. However, their hidden ecological costs have rarely been investigated. We analysed scats (n = 183) from six LGDs and visited Global Positioning System (GPS) location clusters (n = 352) from nine GPS-collared LGDs to reconstruct their diet and assess impacts on wildlife and livestock in Namaqualand, South Africa. Wild mammals, including 10 native species, and small-livestock were the main secondary foods (i.e. besides dog food pellets). A total of 90% of scats and one third of GPS clusters investigated had associated animal remains. When accompanied by a human attendant, fewer LGD scats contained animal matter (39.9%; of which 32.3% wild mammals and 4.6% livestock), in contrast to scats of LGDs on their own (93.2%; 14.4% wild mammals, 75.4% livestock). Similarly, few clusters of accompanied LGDs included animal remains (5.7%; of which 43.8% wild mammals and 31.3% livestock), whereas unaccompanied dogs clustered frequently at carcasses (92.4%; 16% wild mammals, 74% livestock). While sample sizes were relatively small and some dogs might have scavenged, we emphasize the importance of rigorous training and intensive monitoring of LGDs to correct unwanted predation behaviour and to maximize their ecological and protective benefits.