a1_The wide chemical diversity of estrogenic compounds precludes an accurate prediction of estrogenic activity on the basis of chemical structure or radioimmunological assay and thus requires that the potency of these compounds is defined by bioassay. The mammary duct growth response in intact prepubertal and adult gonadectomized female and male mice of the C3H/Di strain was used to assess the estrogenicity of synthetic compounds or their derivatives. The vehicle for tested compounds should be free of estrogenic and other hormonal effects. Olive oil or sunflower oil exerted estrogenic activities and were thus unsuitable as vehicles for the tested compounds. The absence of estrogenic activity, high solubility of different steroid hormones, and the low incidence of the inflammatory reactions at the injection site were achieved by using a vehicle containing benzyl alcohol, benzyl benzoate, butylhydroxyanisole, butylhydroxytoluene, ethyl oleate and ethanol. The bioassay was primarily designed to examine the effect of tested compounds on mammogenesis. The duration of hormone treatment was chosen to be long enough for induction of duct growth but too short to induce lobuloalveolar differentiation. Females were treated for 10 days, males for 15 days. The proportional volume occupied by mammary epithelial structures was estimated by the modified Chalkley's technique. The mean coefficient of variation of quantitative evaluation of 10 different mammary glands obtained by two operators varied between 3.2 and 17.4 %. The mean coefficient of variation of quintuplicate determinations of each mammary gland by one operator was 10.1 %, and 11.1 % by the other. The correlation coefficient between results of two operators was 0.994. Estrogens are primarily defined by their ability to increase the mitotic activity of female secondary sex organs., a2_However, our results have shown that progesterone alone, if administered in a high dose, stimulates mammary growth in both intact prepubertal and OV-X female mice similarly as the synthetic progestatial steroid norethindrone with inherent estrogenic properties. In contrast, progesterone alone had no effect, in young intact or adult castrated males, but norethindrone did stimulate mammary growth. These results demonstrated that the mammary gland of males is a suitable model for estrogen screening., J. Škarda., and Obsahuje bibliografii
This paper presents results from the exam ination of flood embankments by means of three geophysical methods: GPR, mutual impedance of loop antennas measurements and D.C. resistivity method. In order to increase measuring accuracy, the mutual impedance measuring system works at a high frequency. Parameters of mutual impedance measuring system were presented. A method of mutual im pedance measurement results was described. Flood embankments examination results showed that the simultaneous use of few geophysical methods increases accuracy of inhomogeneities detection in near-surface structure of the ground., Remigiusz Mydlikowski, Grzegorz Beziuk and Adam Szynkiewicz., and Obsahuje bibliografické odkazy
Práce informuje o literárních údajích z neuropsychologické literatury a popisuje situaci hlavně v USA, Austrálii, Anglii, Německu a Španělsku. Výzkum a praxe především v posledních dvaceti letech přinesly podstatný pokrok a došlo k vypracování řady kvalitních metod. Jde o problém významný nejen pro forenzní neuropsychologii, ale také pro běžnou klinickou psychologickou praxi. Zkoumání validity odpovědí by se mělo stát i v ČR podstatnou součástí neuropsychologického a psychologického posuzování, umožňuje zpřesňovat nálezy, a tím zlepšovat diagnostiku a další práci s pacienty nebo klienty. Jsou dále podány informace o autorem vyzkoušených metodách., The paper brings information concerning the literary figures from neuropsychological bibli-ography and describes namely the situation in the U.S.A.. Australia, England. Germany, and Spain. The research and practice especially in last twenty years brought essential progress and many high quality methods were elaborated. The problem is significant not only for the fo-rensic neuropsychology but also for the ordinary clinical practice. The examination of validity of responses should become the substantial part of neuropsychological and psychological assess-ment in the Czech Republic, too. It enables to make the findings more precise and thereby to Improve the diagnostics and further work with patients or clients. The information about meth-ods examined by the author is also presented., Jan Preiss., and Obsahuje seznam literatury
The aim of our study was to investigate adrenocortical function in the context of disease activity and inflammatory status in premenopausal RA females. Adrenal glucocorticoid and androgen responses to the 1 μg ACTH 1-24 test were investigated in 23 premenopausal RA and in 15 age- and BMI-matched healthy females. Twelve RA patients were on low-dose prednisone (<8.5 mg/day). Patients with DAS28>3.2 had lower (p<0.05) total plasma cortisol, 17-hydroxyprogesterone, dehydroepiandrosterone and androstenedione responses in the ACTH test compared to healthy controls. Patients with DAS28>3.2 had lower (p<0.05) dehydroepiandrosterone response in the ACTH test compared to patients with DAS28≤3.2. C-reactive protein (CRP), DAS28, and interleukin (IL)-6 negatively correlated with androstenedione response to ACTH 1-24. Responses of all measured adrenal steroids were lower (p<0.05) in patients on low-dose glucocorticoids compared to healthy controls. RA patients not treated with glucocorticoids had lower total cortisol response (p=0.038) but did not differ in free plasma cortisol in the ACTH test. The results indicate an association of increased disease activity with a decrease in adrenal androgen production in RA and normal cortisol bioavailability in patients not treated with glucocorticoids., R. Imrich, M. Vlcek, J. Kerlik, M. Vogeser, F. Kirchhoff, A. Penesova, Z. Radikova, J. Lukac, J. Rovensky., and Obsahuje bibliografii
Diabetes mellitus type 2 ranks among the strongest predictors of cardiovascular diseases (CVD) while the association of type 1 diabetes with CVD is more complex. We studied differences between type 1 and 2 diabetic women regarding association of cardiovascular risk factors with preclinical atherosclerosis expressed as intima-media thickness of common carotid (IMT CCA) and femoral arteries (IMT CFA) measured by high resolution ultrasound. Women with type 1 (n=203) and type 2 diabetes (n=123) were examined with regard to the presence of cardiovascular risk factors. In type 1 diabetic women strong association between IMT CCA and body mass index, waist circumference, and total body fat was found in contrast to type 2 diabetic women. In type 2 diabetic women strong association between IMT CCA and fasting glucose, glycated hemoglobin, and atherogenic index of plasma (log TG/HDL cholesterol) was observed in contrast to type 1 diabetic women. In type 1 diabetic women, IMT CFA was associated with body fat in contrast to type 2 diabetic women. Preclinical atherosclerosis in type 1 diabetic women was strongly associated with factors reflecting body fat and its distribution, while in type 2 diabetic women preclinical atherosclerosis was associated with markers reflecting glucose and lipid metabolic disorders., P. Piťhová, K. Štechová, J. Piťha, V. Lánská, M. Kvapil., and Obsahuje bibliografii
The objective of this work is the chemometric quantification of minerals in rocks. A chemometric method was developed for the determination of chlorite, muscovite, albite and quartz in claystones and clay shales using infrared spectroscopy. Bromide pellets and diffuse reflectance were used to measure the infrared spectra; principa l component analysis and partial least-squares regression were used as chemometric methods. Spectral regions (4000-3000 cm-1 and 1300-400 cm-1) containing important spectral information were chosen by principal compone nt analysis. The calibration models were created by a partial least-squares regression. The mean relative error and relative standard deviation were calculated for the assessment of accuracy and reproducibility. The value of the mean relative error was about 10 % for most of the calibration models. The value of the relative standard deviation ranged from 1.1 to 3.0 % for most calibration models based on diffuse reflectance spectra and from 4.0 to 9.2 % for most calibration models based on spectra obtained with bromide pellets., Michal Ritz, Lenka Vaculíková, Eva Plevová, Dalibor Matýsek and Jiří Mališ., and Obsahuje bibliografické odkazy
It is the objective of this thesis to take into account thermal balance of circulating fluidised bed combustors (CFBC), aiming at efficiency assessment, as regards standards, CSN 07 0302, and DIN 1942. It points to differences between calculations along these two different standards, concerning the solution of problems like different comparative temperatures, to which the calculation of physical losses is related to, varying calculation of mean thermal capacity, or problem of electrical motors input. This article is based on the project GA 617 50 11 solving - "Combine combustion of coal and biomass in fluid boilers"., Zdeněk Kadlec, Bohumir Čech and Jan Matoušek., and Obsahuje bibliografii
EU Emission Trading Scheme, which is applied in EU Member States to decrease CO 2 emissions, requires accurate, reliable, comparable and transparent methodology for monitoring, reporting and verification of emissions. Existing Monitoring and Reporting Guidelines provides operators only with general and undetailed instructions. This paper should provide the plant operators with guidance for a better understanding, interpretation and mastering of the methods of monitoring and reporting CO2 emissions for the purposes of emission trading. This paper is focused on experimental determination of emission and oxidation factors of solid fuels and on calculation of emission factors of gaseous fuels from their composition., Pavel Fott, František Kolář and Dušan Vácha., and Obsahuje bibliografické odkazy
Increased homocysteine levels in serum are typical features of neurodegenerative brain diseases including hydrocephalus. The most frequent therapeutic approach consists of the insertion of a shunt, connecting the brain ventricles to an alternative drainage site. To decide whether the patient should undergo this, the lumbar drainage test is usually carried out to distinguish patients who can benefit from the shunt insertion. In searching for other potential biochemical markers for shunt indication we determined homocysteine levels in CSF during the lumbar drainage test. Homocysteine in CSF was measured during the 5-day lumbar drainage test in 27 patients with normal-pressure hydrocephalus (NPH) and in 25 patients with excluded hydrocephalus. A novelized gas chromatography method with flame ionization detection (GC-FID) was developed and evaluated. During the first two days of lumbar drainage, the levels of CSF homocysteine in NPH patients were significantly higher compared to the controls, while on the fifth day, the homocysteine levels in patients with hydrocephalus reached the level of controls. Determination of CSF homocysteine in patients with confirmed or suspected hydrocephalus may serve as an independent marker for deciding on their further treatment strategy., L. Sosvorová, J. Bešťák, M. Bičíková, M. Mohapl, M. Hill, J. Kubárová, R. Hampl., and Obsahuje bibliografii
The mathematical model and algorithms for calculating the position of GLONASS satellites by means of their broadcast ephemeris is presented in the paper. The algorithms are based on the generalized problem of two fixed centers. One of the advantages of the analytical solution obtained from the generalized problem of two fixed centers is the fact that it embraces perturbations of all orders, from the second and also part ly from the third zonal harmonics (Aksenov, 1969). GLONASS broadcast ephemeris - provided every 30 minutes - contain satellite position and velocities in the Earth fixed coordinate system PZ-90.02 (ICD, 2008), and acceleration due to luni-solar attraction. The GLONASS Interface Control Document recommends that a fourth order Runge-Kutta integration algorithm shall be applied. In the Department of Geomatics (AGH UST) a computer program has been established for fitting position and velocity of GLONASS satellites using their broadcast ephemeris. Intermediate GLONASS satellite orbits are calculated consider ing also the second and third zonal harmonics in the gravitational potential of the Earth. In this paper results of the analytical integration of the equation of the motion of the GLONASS satellites compared to the numerical solution are provided., Władysław Góral and Bogdan Skorupa., and Obsahuje bibliografické odkazy