In this article, the effects of increased light intensities on antioxidant metabolism during ex vitro establishment of Ulmus minor micropropagated plants are investigated. Three month old in vitro plants were acclimatized to ex vitro conditions in a climate chamber with two different light intensities, 200 μmol m-2 s-1 (high light, HL) and 100 μmol m-2 s-1 (low light, LL) during 40 days. Immediately after ex vitro transfer, the increase of both malondialdehyde (MDA) and electrolyte leakage in persistent leaves is indicative of oxidative stress. As the acclimatization continues, an upregulation of the superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) enzyme activities were also observed. Simultaneously, MDA content and membrane permeability stabilized, suggesting that the antioxidant enzymes decrease the deleterious effects of reactive oxygen species (ROS) generation. Unexpectedly, newly formed leaves presented a different pattern of antioxidative profile, with high levels of MDA and membrane leakage and low antioxidant enzyme activity. Despite these differences, both leaf types looked healthy (e.g. greenish, with no necrotic spots) during the whole acclimatization period. The results indicate that micropropagated U. minor plantlets develop an antioxidant enzyme system after ex vitro transfer and that, in general, LL treatment leads to lower oxidative stress. Moreover, new leaves tolerate higher levels of ROS without the need to activate the antioxidative pathway, which suggests that the environment at which leaves are exposed during its formation determinate their ability to tolerate ROS. and M. C. Dias, G. Pinto, C. Santos.
The article approaches the transformation of mobile elite’s
political imagination, linking the emergence of a federative ideology to the impossibility of accommodating minorities. While referring to the case of the Hungarian revolutionary emigration in the middle of the 19th century as an example, the paper examines the categories of “inclusion in” or “exclusion from” a “core-group” as elements determined by the shared imperial legacies and a “minority” status of the public actors. Addressing Harris Mylonas’s scheme of accommodation of minorities within nation-states and combining
it with the concept of “Imperial biographies”, the paper claims that the projects for a Danubian confederation were the results of an inability to address non-homogeneity by a none-core group on a quest for building a nation-state. Driven into exile, Hungarian intellectuals preferred to opt for the incorporation of the other
none-core groups of the Habsburg Empire and their neighbors into a possible confederation that could allow not only to satisfy their aspirations for a national emancipation, but to turn “minorities”
into “majorities”. and Článek zahrnuje poznámkový aparát pod čarou
We describe multicolor photometric solutions of the long-period Algol RX Geminorum, accretion disk kinematics deduced from
phase-dependent radial velocity observations of disk Hα emission and fits to Hα profiles using a non-LTE disk model.
The softer a potential is, the closer the corresponding Keplerian rotation is to a rigid rotation law. Because of the small radial coupling in such a situation the radial mass flow due to viscosity in an α-accretion disk is very inefficient. Here a model is presented in which the mass of an accretion disk in a soft potential well changes this potential in a sense that an efficient mass flow in radial direction becomes possible, thus making the formation of a centra! mass condensation possib!e. This mechanism is of interest in the context of galactic central regions since it is capable of explaining the existence of centra! masses such as observed in the cores of norma! galaxies; it might aiso be of relevance for the explanation of the activity in AGNs.
European larch (Larix decidua Mill.) and Norway spruce [Picea abies (L.) Karst.] synthesize chlorophyll (Chl) in darkness. This paper compares Chl accumulation in 14-d-old dark-grown seedlings of L. decidua and P. abies after shortterm (24 h) feeding with 5-aminolevulinic acid (ALA). We used two ALA concentrations (1 and 10 mM) fed to cotyledons of both species in darkness and in continuous light. The dark-grown seedlings of L. decidua accumulated Chl only in trace amounts and the seedlings remained etiolated. In contrast, P. abies seedlings grown in darkness were green and had significantly higher Chl content. After ALA feeding, higher protochlorophyllide (Pchlide) content was observed in L. decidua than in P. abies cotyledons incubated in darkness. Although short-term ALA feeding stimulated the synthesis of Pchlide, Chl content did not change significantly in cotyledons incubated in darkness. The Chl accumulation in cotyledons fed with ALA was similar to the rate of Chl accumulation in the controls. Higher Chl accumulation was reported in control samples after illumination: 86.9% in L. decidua cotyledons and 46.4% in P. abies cotyledons. The Chl content decreased and bleaching occurred in cotyledons incubated with ALA in light due to photooxidation. Analyses of Chlbinding proteins (D1 and LHCIIb) by Western blotting proved differences between Chl biosynthesis in L. decidua and P. abies seedlings in the dark and in the light. No remarkable increase was found in protein accumulation (D1 and LHCIIb) after ALA application. Our results showed interspecific difference in Chl synthesis between two gymnosperms. Shortterm ALA feeding did not stimulate Chl synthesis, thus ALA synthesis was not the rate-limiting step in Chl synthesis in the dark., N. Maximová, Ľ. Slováková., and Obsahuje bibliografii
Problems related to accuracy of tilt measurements realized during experimental studies of miniature tilt sensors (including MEMS devices) are minutely discussed. The measurements are performed by means of a custom computer controlled test rig, over the full range of pitch and roll. Accuracy of the test rig is determined in terms of uncertainty of the measurements involved. Ways of improving performance of the test rig are briefly introduced. Exemplary results of experimental works are presented. and Obsahuje seznam literatury
This paper presents development of a day ahead load forecasting (DALF) model for Turkish power system with an artificial neural network (ANN). Effects of special holidays including national and religious days, and hourly random load deviations observed in Turkish power system due to significant arc furnace loads are discussed. Performance of the ANN is investigated in the sense of both DALF performance - in terms of both daily mean absolute percentage error (MAPE) and hourly absolute percentage error (APE) - and hourly secondary reserves required to ensure supply/demand adequacy of the system. The most sensitive cities to DALF in terms of daily city temperature forecasts are ranked in order to reduce the input of the developed ANN and thereby to improve execution of the model. Candidate cities are determined based on both their placement with respect to climatic zones of the country and their contribution to the system load during peak hours. The results show that, although a well-trained ANN could provide very satisfactory daily MAPEs at non-special days, such as ~1%, the hourly absolute percentage errors (APE) could be significant due to large random load disturbances, which necessitate special attention during the day ahead allocation of hourly secondary reserves. By limiting the temperature data set with major cities, the input of ANN reduces significantly while not disturbing the MAPEs. Main contributions of the study are; addressing both benefits of the prioritizing the cities in a power system in the sense of their temperature forecast effects on the DALF performance and assessing the performance of DALF in the sense of necessary amount of secondary reserves in power systems which include significant random load deviations (e.g., large arc furnace loads).
The information on the accuracy of harmonic coefficients C1m, S1m as about a non-monotonic function of order m has been obtained from analysis of standard deviations of athe lumped coefficients compited from standart deviations of harmonic coefficients of various recent Earth gravity models as well as from resonant solutions for the individual orders. It is shown that the acuracy is lower for 8< m < 11 than for 12 < m < 15.
A draft of new laser geodynamic twin-satellite is presented. The aim of the experiment, theoretical basis and suggestions to orbit selection - with ~8 to 11 revs. „day^-1 - are described; some comments to a possible design of the satellites and to their separation are also given.
Precise relative gravimeters achieve the internal precision about a few μGal 1, even in field conditions. Nevertheless this precision is in fact concerned with the instant of measurement and can not be confused with the accuracy of the gravity at the gravity station, which is influenced by other effects. The best approach of these two values is question of high-quality elimination of instrumental errors and time-variable disturbing effects affecting the relative gravity measurements., Martin Lederer., and Obsahuje bibliografii
Wire myograph is a device for the in vitro investigation of both, active and passive properties of arteries. Arteries from a variety of animal species, pathological states, and vascular beds were investigated using this method. We focus on the normalization procedure which is aimed to standardize experimental settings and, in part, to simulate physiological conditions. During normalization, it is determined the internal circumference of a vessel stretched to a tension that corresponds to the transmural pressure of 100 mm Hg (IC100). Once it is determined, the internal circumference is traditionally set to (0.9 ⋅ IC100). However, this constant 0.9, called also the normalization factor (NF), was experimentally determined for rat small mesenteric arteries only. Therefore, the aim of our work was to show the influence of different NFs on the passive tension and reactivity of both, rat femoral arteries (FA) an d the first branches of superior mesenteric arteries (MA). We found out that the maximal active wall tension of the FA was achieved at the NF value of 1.1, and that of the MA at 0.9. Considering the values of the active wall tension we suggest that higher reactivity and better signal-to- noise ratio in FA can be achieved when the NF is set at least to 1.0., P. Slezák, I. Waczulíková, P. Bališ, A. Púzserová., and Obsahuje bibliografii