The larval development of the nematode Contracaecum rudolphii (Rudolphi, 1819), a common parasite of the proventriculus of cormorants, was experimentally studied. Within the eggs cultivated in freshwater under laboratory temperatures of 20-22 °C, the developing larva undergoes two moults on days 4-5, attaining the third larval stage. Most of the ensheathed third-stage larvae, 291-457 µm long, hatch spontaneously from egg shells on days 5-6. Experiments have indicated that hatched ensheated third-stage larvae and those still inside egg capsules are already infective to copepods and fishes, which both can be considered paratenic (metaparatenic) hosts. Five copepod species, Acanthocyclops vernalis, Cyclops strenuus, Ectocyclops phaleratus, Eucyclops serrulatus and Megacyclops viridis, the isopod Asellus aquaticus and small carps Cyprinus carpio were infected by feeding them these larvae. In addition, 9 fish species, Alburnoides bipunctatus, Anguilla anguilla, Barbatula barbatula, Cyprinus carpio, Gobio gobio, Perca fluviatilis, Phoxinus phoxinus, Poecilia reticulata and Tinca tinca, were successfully infected by feeding them copepods previously infected with C. rudolphii third-stage larvae. In fishes, larvae from copepods penetrate through the intestinal wall to the body cavity, where, in a few weeks, they become encapsulated; the larvae substantially grow in fish, attaining the body length up to 4.87 mm. In carp fry, the nematode third-stage larvae survived for about 15 months (up to 18 months in fish infected directly, i.e., without copepods). One small cormorant (Phalacrocorax carbo sinensis) was successfully infected by feeding it with copepods harbouring C. rudolphii third-stage larvae.
Extensive experimental investigations were carried out to evaluate the rheological behaviour of fly ash (FA) slurry without and with the addition of bottom ash (BA) and BA slurry without and with the addition of FA. The FA slurries exhibited Bingham behaviour at solid mass concentrations ranging from 60-65% and mixing proportions from 10- 40%. A substantial reduction in yield stress was observed except for mixing proportion of 40% on which the yield stress and viscosity were increased drastically for all solid concentrations. Hence, it can be concluded that the yield stress and viscosity of FA slurry were very much influenced by adding BA up to the mixing proportion of 30%. The rheological behaviour of BA slurries with and without the addition of FA in proportions of 10-50% was investigated and exhibited Newtonian behaviours for solid mass concentrations ranging from 30-50% without and with the addition of FA. The viscosity increases with increasing the solid concentrations and proportion of FA. Based on these experimental data, a correlation was developed to predict the relative viscosity of BA slurries as a function of solid volume fraction and FA mass proportion of 0-50% and the RMSE and R2 values showed good agreement between the experimental and the predicted data.
Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kutzeri oocysts 6 days after ingesting murine tissues. To compare direct and indirect transmissions, two of the kestrels were subsequently directly inoculated with 105 sporulated C. kutzeri oocysts and became patent on days 8 and 9 and shed caryosporan oocysts up to day 25 post inoculation. Additionally, four mice were inoculated with 106 oocysts in order to examine mouse tissues for the presence of developmental stages of C. kutzeri. No coccidian stages were found in the tissues of inoculated mice. The experiment showed that developmental stages of C. kutzeri are able to survive in mouse tissues and cause infection of suitable host after their ingestion.
The polyopisthocotylean Sparicotyle chrysophrii (Van Beneden et Hesse, 1863) was experimentally transmitted to gilthead seabream (Sparus aurata L.) by exposure to eggs (EGT) and by cohabitation with naturally parasitized fish (CT). In EGT trials, the infection was successfully transmitted by introducing containers with monogenean eggs in the fish tanks, with the highest infection level (85.7% prevalence, 3.3 mean intensity) achieved at 6 weeks post exposure (p.e.) to the infection dose of 650 eggs per tank. In CT trials, the progression of the infection was faster and reached higher levels than in EGT. When using small fish juveniles (30 g) (CT-2), infection reached 100% prevalence (mean intensity 8 monogeneans/fish) at 5 weeks p.e., but no eggs could be found in the fish even 10 weeks p.e. By contrast, when larger juveniles (150 g) were used (CT-1), infection levels were lower, but mature adults with eggs were detected starting from 8 weeks p.e. The effect of the parasite on the condition factor, haematocrit, haemoglobin concentration (Hb), red blood cell counts, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin content (MCH) and mean cellular volume (MCV) of infected fish was studied in CT trials. The infection produced hypochromic anaemia, since Hb concentration significantly decreased at 5 and 10 weeks p.e. in CT-2 and at 8 weeks p.e. in CT-1. MCHC was significantly lower in parasitized than in control fish at 5 and 8 weeks p.e. in CT-2 and CT-1, respectively. Also in CT-1, MCH was lower and circulating immature erythrocytes, granulocytes and plasma cells were higher in infected fish than in control ones at 8 weeks p.e. The histopathological effects of the monogenean on the gills of naturally infected fish consisted of lamellar shortening, clubbing and synechiae. The proliferation of the epithelial tissue produced fusion of secondary lamellae, and abundant chloride cells were observed.
Luminiscence (u kovů se častěji hovoří o fotonové emisi) buzená hrotem rastrujícího tunelovacího mikroskopu (STM) kombinuje výhody STM mikroskopu s výhodami optických emisních metod. Hlavním problémem metody je však malý luminiscenční signál. Při tunelujících proudech v řádu jednotek nA je u materiálů s největší kvatovou účinností přeměny elektronů na fotony emitováno do plného prostorového úhlu maximálně ~107 fotonů/s. Velká pozornost proto musí být věnována sběrnému systému fotonů: je zapotřebí maximalizovat prostorový úhel sběru současně s minimalizací ztrát a šumu v detekčním řetězci., Petr Fojtík, Tomáš Mates, Antonín Fejfar, Jan Kočka, Ivan Pelant, Antonín Svoboda., and Obsahuje seznam literatury
The experimental basins in the Jizera Mountains were established at the beginning of the 1980th as a result of the forest devastation due to acid rain and repercussions of following human activities during its disposal with the aim to gain data for the quantification of runoff conditions changes in a changing environment. Seven small catchments with an area from 1.87 km2 to 10.6 km2 are situated in the spring regions in an elevation from 700 m a.s.l. to 1100 m a.s.l. in the catchments of the Černá Nisa, Kamenice, Jizerka and Směda streams. The long-term average annual temperature in the elevation of 780 m a.s.l. is +4.4°C, the long-term annual precipitation sum fluctuates between 1300 mm and 1800 mm. The Jizera Mountains are known for numerous intensive rainfalls in the summer period. The administrator of the experimental basins is the Czech Hydrometeorological Institute (CHMI), Department of Hydrological Research, Experimental Base Jablonec nad Nisou, which performs all operative activities and basic processing of data. In the basins, the monitored elements are water stages and discharges in rivers, surface water quality, rainfall, snow depth and snow water equivalent and climatological parameters. The following contribution gives an overview of information obtained from the hydrological research in the period 1981 - 2004. and Příspěvek se zabývá přehledem poznatků z hydrologického výzkumu v období let 1982-2005 v experimentálních povodích Českého hydrometeorologického ústavu (ČHMÚ) v imisně poškozené oblasti Jizerských hor. Základnu tvoří 7 malých povodí s rozlohou od 1,87 km2 do 10,6 km2 celkové plochy 37 km2 . Všechna povodí leží ve vrcholové části Jizerských hor v Chráněné krajinné oblasti Jizerské hory, na rozvodí řek Labe a Odry. Nadmořská výška povodí se pohybuje mezi 700 až 1100 m. Správcem povodí je Oddělení hydrologického výzkumu, pracoviště Jablonec nad Nisou. Zpočátku byly práce zaměřeny převážně na získání co největšího počtu informací o srážkách, sněhu, množství a jakosti povrchové vody. Po roce 1995 byl monitoring rozšířen na více parametrů hydrologické bilance, sledování klimatických prvků a složek hydrologického procesu. Následně byl upřesňován jejich režim i jejich prostorové rozložení. Byla studována problematika rozdílnosti akumulace a odtávání sněhu na mýtině a v lese a odtoková a kvalitativní odezva na srážkovou činnost a tání sněhu. Za pomoci hydrologických a chemických modelů byly hledány odhady změn jejich závislosti na změně vegetačního pokryvu.
The paper deals with a determination of fundamental parameters of spherical lenses. Novel, experimentally simple and undemanding method describes how to determine the inner parameters of a lens confined between two spherical surfaces. Using the derived formulae one can figure out the lens radii of curvature and its refractive index. Also an uncertainty analysis of calculated parameters is presented. and V článku je řešena problematika určení základních parametrů sférické čočky. Je uvedena nová, experimentálně jednoduchá a nenáročná metoda určení vnitřních parametrů čočky tvořené dvěma sférickými plochami. Pomocí odvozených vztahů je možno vypočítat poloměry křivosti a index lomu materiálu měřené čočky. Také je uveden rozbor nejistot vypočtených hodnot parametrů.
Cylindrical vector beams with radial and azimuthal polarization and optical vortices have increased number of applications in a modern optics. Effective experimental generation of such kind of optical field plays important role in the research process. In this paper, the experimental realization of the basic cylidrical vector beams by spatially structured waveplate is presented. and Cylindrické vektorové svazky s radiální a azimutální polarizací, stejně jako optické víry, představují speciální světelná pole, která nacházejí v moderní optice řadu praktických aplikací. Zvýšený zájem o tyto netradiční struktury je výsledkem jejich stále snadnější a kvalitnější experimentální realizace. V tomto článku je prezentována přímočará generace základních typů cylindrických vektorových svazků pomocí půlvlnné prostorově strukturované fázové destičky.