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2172. Antioxidants aleviate nicotine-induced platelet aggregation in cerebral arterioles of mice in vivo
- Creator:
- Fahim, M. A., Nemmar, A., Singh, S., and Hassan, M. Y.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, trombóza, thrombosis, nicotine, microcirculation, platelets, 14, and 612
- Language:
- English
- Description:
- Experimental data on the effect of nicotine on cerebral microvessel thrombosis is lacking. Therefore, this study was carried out to elucidate the effects of nicotine on platelet aggregation in cerebral (pial) microcirculation of the mouse, and the possible protective effect of vitamins C and E. Male TO mice were divided into six groups, and injected i.p. with saline as a control, nicotine (1 mg/kg), vi tamin C alone (100 mg/kg), vitamin E alone (100 mg/kg), nicotine plus vitamin C or nicotine plus vitamin E, all for one week before the experiment. After one week, platelet aggregation in ce rebral microvessels of these groups of mice were studied in vivo . The appearance of the first platelet aggregation and total blood flow stop in arterioles and venules were timed in seconds. In the animals treated with nicotine, venules did not show any alteration in the platelet aggregation time in comparison to the control animals. However, in arterioles platelet aggreg ation time was significantly accelerated (p<0.001) in nicotine-treated animals as compared to controls. Both vitamins C and E prevented the shortening of arteriolar platelet aggregation ti me significantly (p<0.001) when applied with nicotine but not alone. It can be concluded that nicotine enhances the susceptibility to thrombosis in the cerebral arterioles in vivo and that vitamins C and E have alleviating effect on nicotine-induced thrombotic events in mice pial microvessels., M. A. Fahim ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
2173. Antioxidants and vitamins in clinical conditions
- Creator:
- Zdeněk Zadák, Radomír Hyšpler, Alena Tichá, Miloslav Hronek, Fikrová, P., Jana Rathouská, Dana Hrnčiariková, and Štětina, R.
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, antioxidanty, vitaminy, physiology, antioxidants, vitamins, 14, and 612
- Language:
- English
- Description:
- Various reactive oxygen species (ROS) may be produced from normal biochemical, essential metabolic processes or from external sources as exposure to a variety of agents presented in the environment. Lipids, proteins, carbohydrates and DNA are all capable of reacting with ROS and can be implicated in etiology of various human disorders (rheumatoid arthritis, reperfusion injury, atherosclerosis, lung diseases etc.). In the organism damage by ROS is counteracted with natural antioxidants (glutathione peroxidases, superoxide dismutases, catalase, glutathione, ubiquinol, uric acid, and essential minerals) and nutritional antioxidants from diet (i.e. vitamins E, C, carotenoids). Possible mechanisms of nutritional depletion and side effects of high intake are in the article described., Z. Zadák ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
2174. Antioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa cells HGL5 in vitro
- Creator:
- Kolesárová, Adriana, Michalcová, Katarína, Roychoudhury, Shubhadeep, Baldovská, Simona, Tvrdá, Eva, Vašíček, Jaromír, Chrenek, Peter, Sanislo, Ľuboslav, and Křen, Vladimír
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- flavonoidy, antioxidanty, steroidní hormony, flavonoids, antioxidants, steroid hormons, granulosa cells, transforming growth factor, 14, and 612
- Language:
- English
- Description:
- This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17β-estradiol and progesterone, and human transforming growth factor-β2 (TGF-β2) and TGF-β2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 μg.ml-1 . It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17β-estradiol, progesterone, TGF-β2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 μg.ml-1 , which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 μg.ml-1 ) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells., Adriana Kolesárová, Katarína Michalcová, Shubhadeep Roychoudhury, Simona Baldovská, Eva Tvrdá, Jaromír Vašíček, Peter Chrenek, Ľuboslav Sanisló, Vladimír Křen., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
2175. Antioxidative effect of epigallocatechin gallate against D-galactosamine-induced injury in primary culture of rat hepatocytes
- Creator:
- Moravcová, Alena, Červinková, Zuzana, Kučera, Otto, Mezera, Vojtěch, and Lotková, Halka
- Format:
- print, text, and regular print
- Type:
- model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT
- Subject:
- zvířata, antioxidancia--farmakologie, katechin--analogy a deriváty--farmakologie, buněčné kultury, galaktosamin--farmakologie, glutathion--farmakologie, hepatocyty--účinky léků--patologie, and krysy
- Language:
- English
- Description:
- Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25-10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect. and Alena Moravcová, Zuzana Červinková, Otto Kučera, Vojtěch Mezera, Halka Lotková
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
2176. Antioxidative state of the myocardium and kidneys in acute diabetic rats
- Creator:
- Volkovová, K., Chorváthová, V., Jurčovičová, M., Koszeghyová, L., and Bobek, P.
- Type:
- article, model:article, and TEXT
- Subject:
- rat, streptozotocin, diabetes, antioxidative enzymes, malondialdehyd, and glutathione
- Language:
- English
- Description:
- The influence of acute diabetes (8 days), induced by streptozotocin (45 mg.kg'1 body weight) on myocardial and renal antioxidative conditions was investigated. The animals were given subtherapeutical doses of insulin (Interdep 6 (J. kg'1 body weight, s.c.). Considerably increased levels of malondialdehyde (MDA), as well as of superoxide dismutase (SOD) and catalase (CAT) activity were found in the myocardium of diabetic animals. The oxidized glutathione (GSSG) level and glutathione peroxidase (GSH-PX) activity remained unchanged. The reduced glutathione (GSH) level as well as the activity of glutathione S-transferase (GST) were significantly lower. The activity of GSH-PX in the kidneys of diabetic rats increased by 60 % and that of GST by 105 %, respectively. CAT and SOD activity values were unchanged.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
2177. Antipentylenetetrazol Action of Clobazam in Developing Rats
- Creator:
- Haugvicová, R., Kubová, H., and Mareš, P.
- Type:
- article, model:article, and TEXT
- Subject:
- Clobazam, Pentylenetetrazol, Seizures, Ontogeny, Rat, and Anticonvulsant action
- Language:
- English
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
2178. Antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line A2780
- Creator:
- Caltová, Kateřina and Červinka, Miroslav
- Format:
- braille, text, and regular print
- Type:
- model:article, article, Text, práce podpořená grantem, and TEXT
- Subject:
- nádory vaječníků--farmakoterapie, lidé, ženské pohlaví, buněčné linie nádorové, incidence, cytostatické látky--aplikace a dávkování--terapeutické užití, kombinovaná farmakoterapie, antitumorózní látky--klasifikace, cisplatina, paclitaxel, karboplatina, deoxycytidin, protokoly antitumorózní kombinované chemoterapie, topotekan, etoposid, statistika jako téma, buněčný cyklus, buňky - fyziologické jevy, proliferace buněk--účinky léků, and čas
- Language:
- English
- Description:
- The aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan and etoposide. These cytostatics have a different mechanism of action. To evaluate cytotoxic potential of the tested compounds, the methods measuring various toxicological endpoints were employed including morphological studies, MTT assay, dynamic monitoring of cell proliferation with xCELLigence, cell cycle analysis, caspase 3 activity and expression of proteins involved in cell cycle regulation and cell death. The A270 cell line showed different sensitivity towards the selected cytostatics, the highest cytotoxic effect was associated with paclitaxel and topotecan., Kateřina Caltová, Miroslav Červinka, and Literatura 38
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
2179. Antiradical activity of petals of Philadelphicus coronarius L
- Creator:
- Klečáková, J.
- Type:
- model:article and TEXT
- Language:
- English
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
2180. Antisenescence effect of REAC biomodulation to counteract the evolution of myelodysplastic syndrome
- Creator:
- Maioli, Margherita, Rinaldi, Salvatore, Cruciani, Sara, Necas, Alois, Fontani, Vania, Corda, Giovanna, Santaniello, Sara, Rinaldi, Arianna, Pinheiro Barcessat, Ana Rita, Necasova, Andrea, Castagna, Alessandro, Filipejova, Zita, Ventura, Carlo, and Fozza, Claudio
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- REAC, myelodysplastic syndromes, acute myeloid leukemia, senescence, stem cells, and cellular mechanism
- Language:
- English
- Description:
- About 30 percent of patients diagnosed with myelodysplastic syndromes (MDS) progress to acute myeloid leukemia (AML). The senescence of bone marrow‐derived mesenchymal stem cells (BMSCs) seems to be one of the determining factors in inducing this drift. Research is continuously looking for new methodologies and technologies that can use bioelectric signals to act on senescence and cell differentiation towards the phenotype of interest. The Radio Electric Asymmetric Conveyer (REAC) technology, aimed at reorganizing the endogenous bioelectric activity, has already shown to be able to determine direct cell reprogramming effects and counteract the senescence mechanisms in stem cells. Aim of the present study was to prove if the anti-senescence results previously obtained in different kind of stem cells with the REAC Tissue optimization – regenerative (TO-RGN) treatment, could also be observed in BMSCs, evaluating cell viability, telomerase activity, p19ARF, P21, P53, and hTERT gene expression. The results show that the REAC TORGN treatment may be a useful tool to counteract the BMSCs senescence which can be the basis of AML drift. Nevertheless, further clinical studies on humans are needed to confirm this hypothesis.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public