Number of results to display per page
Search Results
1382. Actigenetic of ACE gene polymorphism in Czech obese sedentary females
- Creator:
- Pavel Suchánek, Jaroslav Hubáček, Ivana K. Lesná, Pinekerová, V., and Věra Adámková
- Type:
- article, články, model:article, and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, biochemie, angiotenzin konvertující enzym, biochemistry, angiotensin converting enzyme, fyzická aktivita, index tělesné hmotnosti, inzerce/delece polymorfismu, physical activity, body mass index, insertion/deletion polymorphism, 2, and 577
- Language:
- English
- Description:
- Obesity is a serious health problem worldwide and many genes have been implicated in determination of obesity, but our knowledge of the genes responsible for individual differences in weight loss after physical intervention are poor. One of the candidate genes is a gene for angiotensin-converting enzyme (ACE) ant its insertion/deletion (I/D) polymorphism. We have analyzed the association between the ACE gene variant in intervened obese females. Twenty four unrelated healthy obese (BMI > 29.9 kg/m2, with abdominal type of obesity) premenopausal (age between 25 and 45 years) Czech Caucasian sedentary and non-diabetic females, pre-selected according the ACE I/D polymorphism (twelve II and twelve DD homozygotes) were studied in a medical research centre. They underwent 9 weeks intervention program (combination of the lowering of dietary intake to optimal level for the age and 3 times a week physical activity at fitness centre). The participants were supervised to sustain a heart rate of 65 % of maximum. Anthropometrical, biochemical parameters and body composition (Bodystat 1500) were analyzed before and after the intervention. Our study suggest, that in Czech Caucasian females I/D polymorphism within the ACE gene will have no major effect on weight loss. Interestingly, we have detected, that in obese females II genotype was associated with higher increase in basal metabolic rate (202 kcal per day) then in DD homozygotes (p<0.05), thus at least under some circumstances, this genetic variant may have an slight effect on BMI development., P. Suchánek ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1383. Actin and major sperm protein in spermatozoa of a nematode, Graphidium strigosum (Strongylida: Trichostrongylidae)
- Creator:
- Mansir, Aicha and Justine, Jean-Lou
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- actin, major-sperm-protein, cytoskeleton, spermatozoon, immunocytochemistry, and nematode
- Language:
- English
- Description:
- In most amoeboid cells, the main protein involved in motility is actin. Nematode sperm are an exception, and their amoeboid motility is based on major sperm protein (MSP). We have studied the localization of actin and MSP in spermatids and spermatozoa of Graphidium strigosum (Dujardin, 1845), a species which has elongate male germ cells in which organelles are easily identified. Electrophoreses of G. slrigosum sperm proteins indicate that the main protein band, about 15 kDa in molecular weight, is specifically recognized by an anti-MSP polyclonal antibody developed against MSP of Caenorhabditis elegans (Burke and Ward 1983). Actin is present in small quantities. Immunocytochemical observations reveal that actin and MSP have an identical localization in precise areas of the male germ cells. Spermatids are labelled as dots around a central unlabelled zone, and spermatozoa are labelled only at the level of the anterior cap. Observations in G. strigosum are similar to that previously obtained in Heligmosomoides polygyrus (Mansir and Justine 1996). Co-localization of actin and MSP in the anterior cap of the spermatozoon, the region associated with pseudopod production, does not demonstrate directly that actin is involved in amoeboid movements, but shows that the role of actin in the cytoskeleton of nematode sperm should be re-investigated.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1384. Acting on behalf of the Bohemian King: Ferdinand Ernst von Waldstein's Journey to Westphalia (1645-1647) /
- Creator:
- Hrbek, Jiří,
- Type:
- text and studie
- Subject:
- Mezinárodní vztahy, světová politika, <<z >>Valdštejna, Ferdinand Arnošt,, mír vestfálský (1648), vztahy mezinárodní, politika mezinárodní, diplomacie, jednání mírová, české země 1620-1740, zahraniční politika, mezinárodní vztahy, and světové dějiny 1492-1648
- Language:
- English
- Rights:
- unknown
1385. Action of fenoxycarb on metamorphosis and cocoon spinning in Chrysoperla carnea (Neuroptera: Chrysopidae): identification of the JHA-sensitive period
- Creator:
- Bortolotti, Laura, Micciarelli Sbrenna, Anna, and Sbrenna, Giovanni
- Type:
- article, model:article, and TEXT
- Subject:
- Chrysoperla carnea, juvenoids, fenoxycarb, development, metamorphosis, and JH sensitive period
- Language:
- English
- Description:
- Fenoxycarb is an insecticide with a juvenile hormone mimicking effect, which disturbs metamorphosis in several insect species, including the neuropteran Chrysoperla carnea. Administration of different doses of fenoxycarb to third instar larvae causes a dose dependent inhibition of metamorphosis and cocoon spinning. When treated within 48 h of the last larval ecdysis, this insect shows a temporary inhibition of metamorphosis and cocoon spinning, which leads to a prolongation of the third larval instar. When treated after 60 h into the last instar, most of larvae were unable to metamorphose and spin a cocoon. Thus, C. carnea is most sensitive to this juvenoid in the period after 60 h into the last larval instar.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
1386. Action of GABA-B antagonist on cortical epileptic afterdischarges in rats is similar to that of GABA-A antagonist
- Creator:
- Živanović, D., Klára Bernášková, Kaminskij, Yu., and Pavel Mareš
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, epileptické záchvaty, mozková kůra, epileptic seizures, cerebral cortex, rat, CGP 35348, bicuculline, 14, and 612
- Language:
- English
- Description:
- Threshold intensities for epileptic phenomena induced by cortical stimulation were used for comparison of the action of GABA-B and GABA-A antagonists in rats with implanted electrodes. Both CGP 35348 (200 mg/kg i.p.) and bicuculline (4 mg/kg i.p.) significantly decreased thresholds for spike-and-wave afterdischarges and their motor counterpart (clonic seizures) whilst transition into the second, limbic type of afterdischarge as well as threshold for movements directly bound to stimulation remained uninfluenced by either drug., D. Živanović, K. Bernášková, Yu. Kaminskij, P. Mareš., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1387. Action of granulopoiesis-stimulating cytokines rhG-CSF, rhGM-CSF, and rmGM-CSF on murine hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC).
- Creator:
- Hofer, M., Vacek, A., and Weiterová, L.
- Type:
- article, model:article, and TEXT
- Subject:
- Murine hematopoiesis, GM-CFC, rhGM-CSF, and rmGM-CSF
- Language:
- English
- Description:
- The aim of this study was to provide new data to the knowledge of mechanisms by which recombinant human granulocyte colony-stimulating factor (rhG-CSF), recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and recombinant murine granulocyte macrophage colony-stimulating factor (rmGM-CSF) enhance the numbers of colonies growing from hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) in the murine bone marrow. The in vitro technique for cultivating GM-CFC from normal bone marrow cells was used. For evaluation of stimulatory actions of the drugs studied, the factors themselves or sera of mice given these factors were added to the cultures. The factors or the sera were present in the cultures either as the only potentially stimulatory agents or acted jointly with a suboptimum concentration of recombinant murine interleukin-3 (rmIL-3). It was found that both rhG-CSF and rmGM-CSF stimulate the proliferatio n of GM-CFC by a combination of direct mechanisms (direct actions on the target cells) and indirect effects (effects mediated through the induction of other cytokines and/or growth factors in the murine organism). The rhGM-CSF exhibited somewhat weaker in vitro effects in comparison with the other two factors and only indirect effects were noted. Additional in vivo experiments documented that, in spite of differences in mechanisms of action of the individual drugs studied on murine bone marrow cells in vitro, equal in vivo doses of the factors induce quantitatively similar effects on the production of GM-CFC in vivo.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1388. Action of Two Neuroactive Steroids Against Motor Seizures Induced by Pentetrazol in Rats During Ontogeny
- Creator:
- Mareš, P., Haugvicová, R., and Kasal, A.
- Type:
- article, model:article, and TEXT
- Subject:
- Convulsion, Rat, Development, Pregnanolone, New neuroactive steroid, Anticonvulsant action, and Time course of action
- Language:
- English
- Description:
- The anticonvulsant action of two neuroactive steroids, 3α–hydroxy-5β–pregnan-20-one (pregnanolone) and triethylammonium 3α–hydroxy-20-oxo-5α–pregnan-21-yl hydrogensuccinate (THDOC-conjugate), was tested against motor seizures induced by pentetrazol in immature rats. Five age groups (7, 12, 18 and 25 days old and adult rats) were pretreated with the steroids in doses from 2.5 to 40 mg/kg i.p. Twenty minutes later pentetrazol (100 mg/kg s.c.) was administered. Minimal seizures (clonic seizures of head and forelimb muscles with preserved righting ability) could be induced in the three older age groups. They were suppressed by pregnanolone in all these tested groups (this effect was best expressed in 18-day-old rats and decreased with age), whereas significant changes in THDOC-conjugate-pretreated animals appeared only in 18-day-old rats. Generalized tonic-clonic seizures were suppressed by both neuroactive steroids in all age groups, this effect being more marked with pregnanolone and again decreased with age. The 7- and 12-day-old rats exhibited higher sensitivity of the tonic phase so that generalized clonic seizures were observed. Duration of the effect was studied in 12- and 25-day-old animals; it was substantially shorter in the older rats than in 12-day-old animals. Both drugs exhibited an anticonvulsant action in developing rats but, unfortunately, their effect was only shortlasting.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1389. Actions of angiotensin II and dopamine in the medial preoptic area on prolactin secretion
- Creator:
- Leite, C. M., Machado, G. J. R., Dornelles, R. C. M., and Franci, Celso Rodrigues
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, dopamin, estrogeny, physiology, dopamine, estrogens, prolactin, medial preoptic area, angiotensin II, dopaminergic antagonists, 14, and 612
- Language:
- English
- Description:
- Dopamine (DA) is known as a primary regulator of prolactin secretion (PRL) and angiotensin II (Ang II) has been recognized as one brain inhibitory factor of this secretion. In this work, estrogen-primed or unprimed ovariectomized rats were submitted to the microinjection of saline or Ang II after previous microinjection of saline or of DA antagonist (haloperidol, sulpiride or SCH) both in the medial preoptic area (MPOA). Our study of these interactions has shown that 1) estrogen-induced PRL secretion is mediated by Ang II and DA actions in the MPOA, i.e. very high plasma PRL would be prevented by inhibitory action of Ang II, while very low levels would be prevented in part by stimulatory action of DA through D2 receptors, 2) the inhibitory action of Ang II depends on estrogen and is mediated in part by inhibitory action of DA through D1 receptors and in other part by inhibition of stimulatory action of DA through D2 receptors., C. M. Leite, G. J. R. Machado, R. C. M. Dornelles, C. R. Franci., and Obsahuje bibliografii a bibliografické poznámky
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1390. Actions of lyotropic anions on the mechanical properties of fast and slow twitch rat muscles at different temperatures
- Creator:
- Wondmikun, Y., Tomáš Soukup, and Gerhard Asmussen
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, svaly, teplota, muscles, temperature, slow and fast muscles, contractile properties, twitch potentiation, lyotropic anions, rat, 14, and 612
- Language:
- English
- Description:
- The effects of lyotropic (swelling) anions (Cl-, Br-, NO3- and I-) on contractile properties of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscles were investigated in vitro at 20 °C and 35 °C. Isolated muscles bathed in anionic Tyrode solution were stimulated directly and isometric single twitches and fused tetanic contractions were recorded. In a Cl- Tyrode solution a decrease of the bathing temperature led to a cold potentiation of the twitch tension (Pt) in EDL muscles, however, to a cold depression in SOL muscles, in both muscles combined with a prolongation of contraction (CT) and half relaxation (HRT) times. The extent and order of the potentiating effect of lyotropic anions on the Pt, CT and HRT in EDL and SOL were quite similar and increased in the order: Cl-< Br- < NO3- < I-. Since the lyotropic anions did not influence tetanic tensions, the twitch-tetanus ratio (TTR) was increased in NO3- and I- solutions. All effects of the anions were rapidly and completely reversed in both muscles when the test solution was replaced by the normal one. The temperature decrease caused no significant alteration in the potentiation capacity of the anions or in the kinetics of their action and reversibility., Y. Wondmikun, T. Soukup, G. Asmussen., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public