This paper presents some structural properties of a generalized Petri net (PN) with an algorithm to determine the (partial) conservativeness and (partial) consistency of the net. A product incidence matrix A=CCT or A~=CTC is defined and used to further improve the relations among PNs, linear inequalities and matrix analysis. Thus, based on Cramer's Rule, a new approach for the study of the solution of a linear system is given in terms of certain sub-determinants of the coefficient matrix and an efficient algorithm is proposed to compute these sub-determinants. The paper extends the common necessary and/or sufficient conditions for conservativeness and consistency in previous papers and some examples are designed to explain the conclusions finally.
Toxoplasma gondii (Nicolle et Manceaux, 1908) is an obligate intracellular apicomplexan parasite and can infect warmblooded animals and humans all over the world. Development of effective vaccines is considered the only ideal way to control infection with T. gondii. However, only one live vaccine is commercially available for use in sheep and goats. Thus more effective antigenic proteins are searched for. In the present study we report a novel protein by secreted T. gondii termed Myc regulation 1 (MYR1). The physical and chemical characteristics, epitopes, hydrophilicity and functional sites of MYR1 were analysed by multiple bioinformatic approaches. The 3D models of MYR1 proteins were constructed and analysed. Furthermore, liner B-cell epitopes and T-cell epitopes of MYR1 protein and SAG1 were predicted. Compared to SAG1, MYR1 with good B-cell epitopes and T-cell epitopes had a potentiality to become a more successful vaccine against T. gondii. The bioinformatics analysis of MYR1 proteins could laid the foundation for further studies of its biological function experimentally and provide valuable information necessary for a better prevention and treatment of toxoplasmosis., Jian Zhou, Gang Lu, Shenyi He., and Obsahuje bibliografii
Repeated geodetic observations were applied in mining location Louky near Karviná to detect surface changes from undermining in complex geo-mechanical conditions. Analyses of the subsidence magnitude and the length and direction of horizontal displacements showed that the subsidence trough was formed unevenly not only due to the position of the exploited local longwall panels and their different size but also showed a notable effect of the dominant tectonic fault. The significantly uneven development of the subsidence trough negatively affects line constructions. Terrain deformations of the road and stream pipeline were computed and classified into the building site categories according to the Czech standard ČSN 73 0039. The course of individual deformations in the monitored locality is influenced by a complex geo-mechanical situation. While the course of the subsidence curves is continuous at the observed pipeline and the classification of individual sections into building site categories corresponds with that, the road profile points out a more complex development of surface deformations. At the end of the observed period, 5 % of the profiles’ sections fell into category III (medium intensity), 49 % into category IV (moderate intensity) and 32 % stayed in category V (very moderate intensity of mining effects).
Using GNSS method, fixed points of an observation network were repeatedly surveyed on the surface of the undermined area. Below the surface, at the depth of c. 1 km, there were four mining panels exploited subsequently. The main reaction of the surface points to the changes in the rock massif and the movement of the points were different, according to their surface position, local geo-mechanical conditions etc. This paper analyses the time-dependence of the surface points mining subsidence and horizontal movements on the progress of the exploitation., Hana Doležalová, Vlastimil Kajzar, Kamil Souček and Lubomír Staš., and Obsahuje bibliografické odkazy
The aim of this study was to determine the accuracy and precision of McMaster method with Raynaud's modification in the detection of the eggs of the nematodes Toxocara canis (Werner, 1782) and Trichuris ovis (Abildgaard, 1795) in faeces of dogs. Four variants of McMaster method were used for counting: in one grid, two grids, the whole McMaster chamber and flotation in the tube. One hundred sixty samples were prepared from dog faeces (20 repetitions for each egg quantity) containing 15, 25, 50, 100, 150, 200, 250 and 300 eggs of T. canis and T. ovis in 1 g of faeces. To compare the influence of kind of faeces on the results, samples of dog faeces were enriched at the same levels with the eggs of another nematode, Ascaris suum Goeze, 1782. In addition, 160 samples of pig faeces were prepared and enriched only with A. suum eggs in the same way. The highest limit of detection (the lowest level of eggs that were detected in at least 50% of repetitions) in all McMaster chamber variants were obtained for T. canis eggs (25-250 eggs/g faeces). In the variant with flotation in the tube, the highest limit of detection was obtained for T. ovis eggs (100 eggs/g). The best results of the limit of detection, sensitivity and the lowest coefficients of variation were obtained with the use of the whole McMaster chamber variant. There was no significant impact of properties of faeces on the obtained results. Multiplication factors for the whole chamber were calculated on the basis of the transformed equation of the regression line, illustrating the relationship between the number of detected eggs and that of the eggs added to the sample. Multiplication factors calculated for T. canis and T. ovis eggs were higher than those expected using McMaster method with Raynaud modification.
The growth and reproductive biology of the invasive goldfi sh Carassius auratus auratus were studied in Lake Trasimeno, central Italy. The results of the research revealed that the population is made up of eight age-classes. The sex ratio proved to be extremely unbalanced (1 : 19 in favour of females). Growth can be deemed rapid, the von Bertalanffy growth in length function being TL = 43.019{1- e(-0.272(t+0.162) )} for the total sample and Ф’ = 2.702. No sexual dimorphism in growth was observed. Back-calculation analysis suggested the existence of an inverse Lee phenomenon among 1 year-old specimens. The reproductive period covers a broad time-span, from March to June. In females, sexual maturity is reached after the second winter of life (2+ age-class); however, a small percentage (7.55%) of females is able to reproduce at the age of 1 year. Most of the males attained sexual maturity in the fi rst year (60.61%). The reproductive investment of the females is high; the relationship between SL and the number of eggs was Ne = 0.0041 SL4.368. Fecundity varied from 286 to 219104 eggs, with an average relative fecundity of 103 ± 5 eggs g-1; the mean diameter of the eggs was 1.27 ± 0.01 mm. The reproductive investment of the females was not homogeneous across the ageclasses; in addition to absolute fecundity, relative fecundity and egg diameter were seen to increase with the size of the specimens.
Judging the influence of private law enforcement on the success of the leniency programs is a veryinteresting and current topic. When dealing with it, it is necessary to find an answer to following question:How should be handled the initiative of the European Commission to strengthen the private law pillar ofthe cartel law enforcement not to weaken the public law pillar at the same time? It is possible to assume thatthe support of private enforcement of damages discourages cartel participants to report the existence of acartel and avoid the fine imposed by the state, because by admitting a cartel they potentially face an evenlarger financial burden than in the case of a fine. However, the other point of view is, taking into account thevery low effort of the impaired parties (mainly consumers) to enforce their claims through private means,that the support of private enforcement should not be perceived as a danger to the functionality of leniencyprograms, but only as their suitable complement, which can exert sufficient pressure on the cartel participantsto perform their activities in accordance with law. The article strives to show that at the moment the supportof private enforcement of cartel law does not pose any danger to leniency programs and that the implementationof legal institutions proposed by the European Commission, which would emphasize the role of privateenforcement, is desirable.
There are three basic procedures used for an assessment of the electrical heart field from the body surface: standard electrocardiography, vectorcardiography, and body surface potential mapping (BSPM). BSPM has two major advantages over both other methods: 1) it allows exploring the entire chest surface, thus providing all the information on the cardiac electric field available at the body surface; 2) it is more sensitive in detecting local electrical events, such as local conduction disturbances or regional heterogeneities of ventricular recovery. Nevertheless the results obtained using BSPM procedure cannot answer all questions about real causality of detected changes of the electrical heart field. We tried therefore to use mathematical model of the electrical field in order to answer these questions. A simple and anatomical forward calculation model was used to test the hypothesis whether the altered position of the heart could explain heterogeneity of repolarization at late stages of pregnancy in humans. The hypothesis was declined. Further findings included: A. Repolarization duration (represented by QT interval) in healthy subjects are distributed regularly and predictably on the body surface carrying no information about local pathology. B. At any systemic analysis of ventricular repolarization, it is vital to consider the regions where any electrode systems record low amplitudes due to methodological, not pathological reasons. C. Anatomical (heterogeneous) model did not yield superior results over simple (homogenous) ones possibly since none reflected the specific torso geometry of individual patients., O. Kittnar, M. Mlček., and Obsahuje bibliografii a bibliografické odkazy
Over a large range of the pressure, one cannot ignore the fact that the viscosity grows significantly (even exponentially) with increasing pressure. This paper concerns long-time and large-data existence results for a generalization of the Navier-Stokes fluid whose viscosity depends on the shear rate and the pressure. The novelty of this result stems from the fact that we allow the viscosity to be an unbounded function of pressure as it becomes infinite. In order to include a large class of viscosities and in order to explain the main idea in as simple a manner as possible, we restrict ourselves to a discussion of the spatially periodic problem.