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1842. The draft genome sequence of the Japanese honey bee, Apis cerana japonica (Hymenoptera: Apidae)
- Creator:
- Yokoi, Kakeru, Uchiyama, Hironobu, Wakamiya, Takeshi, Yoshiyama, Mikio, Takahashi, Jun-Ichi, Nomura, Tetsuro, Furukawa, Tsutomu, Yajima, Shunsuke, and Kimura, Kiyoshi
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- blanokřídlí, včelovití, Hymenoptera, Apidae, Apis cerana japonica, genome sequence, transposable elements, innate immune genes, 2, and 59
- Language:
- English
- Description:
- Honey bees are not only important for honey production but also as pollinators of wild and cultivated plants. The Eastern honeybee (Apis cerana) is more resistant to several pathogens than the Western honeybee (Apis mellifera), and the genomes of two strains of the nominotypical subspecies, A. cerana cerana, northern (Korea) and southern (China) strains, have been sequenced. Apis cerana japonica, another subspecies of A. cerana, shows many specific features (e.g. mildness, low honey production and frequently absconds) and it is important to study the molecular biological and genetic aspects of these features. To accelerate the genetic research on A. cerana japonica, we sequenced the genome of this subspecies. The draft genome sequence of A. cerana japonica presented here is of high quality in terms of basic genome status (e.g. N50 is 180 kbp, total length is 211 Mbp, and largest contig length is 1.31 Mbp) and BUSCO results. The gene set of A. cerana japonica was predicted using AUGUSTUS software and the set of genes was annotated using Blastp and InterProScan, and GO terms were added to each gene. The number of genes is higher than in A. mellifera and in the two strains of A. cerana cerana sequenced previously. A small number of transposable elements and repetitive regions were found in A. cerana japonica, which are also in the genomes of A. mellifera and the northern and southern strains of A. cerana cerana. Apis cerana is resistant to several pathogens that seriously damage A. mellifera. We searched for 41 orthologs related to the IMD and Toll pathways, which have key roles in the immune reaction to invading pathogens. Some orthologs were not identified in the genome of the northern strain of A. cerana cerana. This indicates that the Toll and IMD pathways function in the same way as in A. mellifera and Drosophila melanogaster., Kakeru Yokoi, Hironobu Uchiyama, Takeshi Wakamiya, Mikio Yoshiyama, Jun-Ichi Takahashi, Tetsuro Nomura, Tsutomu Furukawa, Shunsuke Yajima, Kiyoshi Kimura., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
1843. The earliest fossil flower bugs (Heteroptera: Cimicomorpha: Cimicoidea: Vetanthocoridae) from the Middle Jurassic of Inner Mongolia, China
- Creator:
- Hou, Wenjing, Yao, Yunzhi, Zhang, Weiting, and Ren, Dong
- Type:
- article, články, model:article, and TEXT
- Subject:
- Zoologie, zoologie, entomologie, Heteroptera, Vetanthocoridae, fossil, Middle Jurassic, Inner Mongolia, 2, and 59
- Language:
- English
- Description:
- One new genus with two new fossil species, Pumilanthocoris gracilis gen. n. sp. n. and P. obesus gen. n. sp. n., which were found in the Middle Jurassic Jiulongshan Formation of Inner Mongolia, China, are described and illustrated. These are the earliest fossil records of Vetanthocoridae., Wejing Hou ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
1844. The effect of ambient temperature on larvae of Scatopsciara cunicularius (Diptera: Sciaridae) feeding on the thallose liverwort Marchantia polymorpha
- Creator:
- Sawangproh, Weerachon, Ekroos, Johan, and Cronberg, Nils
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, dvoukřídlí, smutnicovití, Diptera, Sciaridae, Scatopsciara cunicularius, gnat larva, sciarid fly, biological control, Marchantia polymorpha, 2, and 59
- Language:
- English
- Description:
- Herbivory on liverworts is rarely reported. We studied the effects of feeding by larvae of the sciarid fly Scatopsciara cunicularius on the growth of the thalloid liverwort Marchantia polymorpha at two different constant temperatures, 12°C and 22°C. Larvae reared at the lower temperature fed slower and over a longer period of time, which resulted in more damage and a greater reduction in the growth of the liverwort than that caused by those reared at the higher temperature. The reduction in growth of the liverwort was positively density-dependent in terms of number of larvae at both temperatures. These results indicate that the larvae of S. cunicularius are likely to be an effective means of controlling M. polymorpha, which is a common weed in plant nurseries and greenhouse cultures., Weerachon Sawangproh, Johan Ekroos, Nils Cronberg., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1845. The effect of C-type nautriuretic peptide on delayed rectifier potassium currents in gastric antral circular myocytes of the guinea-pig
- Creator:
- Xu, H. Y., Huang, X., Yang, M., Sun, J.-B., Piao, L.-H., Zhang, Y., Gao, L., and Xu, Wen Xie
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Obecná zoologie, fyziologie živočichů, natriuretické peptidy, peptid, animal physiology, natriuretic peptides, stomach, delayed rectifier potassium currents, C-type natriuretic peptide, gastric myocytes, cyclic GMP, 2, and 591
- Language:
- English
- Description:
- C-type natriuretic peptides (CNP) play an inhibitory role in smooth muscle motility of the gastrointestinal tract, but the effect of CNP on delayed rectifier potassium currents is still unclear. This study was designed to investigate the effect of CNP on delayed rectifier potassium currents and its mechanism by using conventional whole-cell patch- clamp technique in guinea-pig gastric myocytes isolated by collagenase. CNP significantly inhibited delayed rectifier potassium currents [IK (V)] in dose-dependent manner, and CNP inhibited the peak current elicited by depolarized step pulse to 86.1±1.6 % (n=7, P<0.05), 78.4±2.6 % (n=10, P< 0.01) and 67.7±2.3 % (n=14, P<0.01), at concentrations of 0.01 μmol/l, 0.1 μmol/l and 1 μmol/l, respectively, at +60 mV. When the cells were preincubated with 0.1 μmol/l LY83583, a guanylate cyclase inhibitor, the 1 μmol/l CNP-induced inhibition of IK (V) was significantly impaired but when the cells were preincubated with 0.1 μmol/l zaprinast, a cGMP-sensitive phosphodiesterase inhibitor, the 0.01 μmol/l CNP-induced inhibition of IK (V) was significantly potentiated. 8-Br-cGMP, a membrane permeable cGMP analogue mimicked inhibitory effect of CNP on IK (V). CNP-induced inhibition of IK (V) was completely blocked by KT5823, an inhibitor of cGMP-dependent protein kinase (PKG). The results suggest that CNP inhibites the delayed rectifier potassium currents via cGMP-PKG signal pathway in the gastric antral circular myocytes of the guinea-pig., H. Y. Xu, X. Huang, M. Yang, J.-B. Sun, L.-H. Piao, Y. Zhang, L. Gao, W.-X. Xu., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1846. The effect of CaCl2 on calcium content, photosynthesis, and chlorophyll fluorescence of tung tree seedlings under drought conditions
- Creator:
- Li, Z., Tan, X. F., Lu, K., Liu, Z. M., and Wu, L. L.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorid vápenatý, výměna plynu, calcium chloride, gas exchange, Vernicia fordii, water stress, 2, and 581
- Language:
- Multiple languages
- Description:
- The study investigated the effects of different CaCl2 concentrations (2, 5, and 10 mM) on photosynthetic enzymatic activities, photosynthesis, and chlorophyll fluorescence of tung tree seedlings under drought conditions. Plants were sprayed with either CaCl2 or distilled water until run-off. Irrigation was then withheld to induce drought stress. The strength of drought stress was evaluated by relative leaf water content and soil water content, which was 27.3 and 9.5% on day 0 and day 12, respectively. Drought stress decreased activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase, chlorophyll (a+b) content, net photosynthetic rate, stomatal conductance, transpiration rate, electron transport rate, the maximal quantum yield of PSII photochemistry, and effective quantum yield of PSII in tung tree seedlings. The CaCl2 pretreatments alleviated the negative effect of drought stress to some degree on all the parameters mentioned above., Z. Li, X. F. Tan, K. Lu, Z. M. Liu, L. L. Wu., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1847. The effect of disturbance caused by rivers flooding on ground beetles (Coleoptera: Carabidae)
- Creator:
- Sienkiewicz, Pawel and Zmihorski, Michal
- Type:
- article, články, model:article, and TEXT
- Subject:
- Zoologie, zoologie, entomologie, Carabidae, floodplain, flooding, disturbance, ground beetles, river ecology, biodiversity, 2, and 59
- Language:
- English
- Description:
- We studied the effect of flooding on a carabid community inhabiting grassland in a large river valley (W Poland). We used pitfall-traps to catch beetles from April to November 1999–2001. Some of the samples collected were preceded by floods during the collecting period, which enabled us to evaluate the effects of flooding on species composition and abundance. We collected 17,722 individuals belonging to 108 species. The number of species and individuals per sample differed between plots and showed a nonlinear decrease over time, from spring to autumn. Carabids were more abundant in samples collected after floods than in the control samples. In contrast, the expected cumulative number of species as a function of the number of individuals collected was lower in samples collected after floods than in the control samples. In the case of the most abundant species the species-specific responses in terms of the numbers caught after flooding differed, with those of (e.g. Agonum micans) increasing and those of (Amara plebeja, Epaphius secalis) decreasing. This study shows that floods filter the community with the result that some species increase in abundance but the overall species richness decreases. Therefore natural floods are important in shaping the structure of communities of epigeic carabids on floodplains., Pawel Sienkiewicz, Michal Zmihorski., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
1848. The effect of galling aphids feeding on photosynthesis photochemistry of elm trees (Ulmus sp.)
- Creator:
- Kmieć, K., Rubinowska, K., Michałek, W., and Sytykiewicz, H.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- fotosyntéza, mšice, jilm, photosynthesis, aphids, Ulmus, biotic stress, Eriosomatinae, fluorescence parameters, host plant response, 2, and 581
- Language:
- Multiple languages
- Description:
- Changes of chlorophyll (Chl) a fluorescence and photosynthetic pigment contents were analysed in galled leaves (visibly damaged and undamaged parts) and intact leaves. The values of minimal fluorescence of the dark-adapted state, maximal quantum yield of PSII photochemistry, effective quantum yield of PSII photochemical conversion, and photochemical quenching coefficient decreased in Ulmus pumila L. leaves galled by Tetraneura ulmi (L.) and in U. glabra Huds. galled by Eriosoma ulmi (L.). Colopha compressa (Koch.) feeding affected these parameters only in damaged parts of U. laevis Pall. galled leaves. The increasing number of T. ulmi galls progressively decreased photosynthetic performance. In gall tissues of all analysed aphid species, the lowest photosynthetic pigment content was found, indicating that the photosynthetic capacity must have been low in galls. Significant reduction of Chl and carotenoid contents were observed in damaged and undamaged portions of galled leaves only in the case of T. ulmi feeding., K. Kmieć, K. Rubinowska, W. Michałek, H. Sytykiewicz., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1849. The effect of hay mulching on soil temperature and the abundance and diversity of soil-dwelling arthropods in potato fields
- Creator:
- Dudás, Péter, Menyhárt, László, Gedeon, Csongor, Aambrus, Gergely, and Tóth, Ferenc
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, brambory, teplota půdy, potatoes, soil temperature, potato field, soil-dwelling arthropods, hay mulch, soil sampler, soil pin trap, 2, and 59
- Language:
- English
- Description:
- The average soil temperature was significantly lower in plots covered with hay. Hay mulching, however, did not increase significantly the total number of soil micro-arthropods collected using a soil pin trap and a soil sampler. Significant increases in the number of individuals was recorded only for certain groups. 64% of all the arthropods collected using soil pin traps were collected in hay-covered plots and 36% in control plots. This increase was statistically significant for the orders Entomobryomorpha and Poduromorpha of the subclass Collembola and surface-dwelling (epigeic) Coleoptera. For the samples collected using the soil sampler, 57% of the specimens were collected from hay-covered plots and 43% from control plots. As for the pin traps this was reflected in differences in the numbers of the orders Entomobrhyomorpha and Poduromorpha of the subclass Collembola and Pauropoda, collected in the treated and control plots. We conclude that mulching affected the different arthropod groups differently., Péter Dudás, László Menyhárt, Csongor Gedeon, Gergely Aambrus, Ferenc Tóth., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1850. The effect of hyperthermia in vitro on vitality of rabbit preimplantation embryos
- Creator:
- Alexander Makarevič, Olexiikova, L., Peter Chrenek, Elena Kubovičová, Fréharová, K., and Juraj Pivko
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Obecná zoologie, fyziologie živočichů, králíci, embryogeneze živočichů, hypertermie, apoptóza, animal physiology, rabbits, animal embryology, hyperthermia, apoptosis, Hsp70, actin, 2, and 591
- Language:
- English
- Description:
- The aim of our study was to test the in fluence of short exposure (6 h) of preimplantation rabbit embryos to elevated temperatures (41.5 ºC or 42.5 ºC) in vitro on their developmental capacity. Fertilized eggs recovered from female oviducts at the pronuclear stage (19 hpc) were cultured at standard temperature (37.5 ºC) until the morula stage (72 hpc). Afterwards, the embryos were divided into two groups, cultured for 6 h either at hyperthermic (41.5 ºC or 42.5 ºC) or standard temperature (control 37.5 ºC), post-incubated overnight (16-20 h) at 37.5 ºC and then evaluated for developmental stages, apoptosis (TUNEL), proliferation (cell number), actin cytoskeleton and presence of heat-shock proteins Hsp70. It was observed that hyperthermia at 41.5 ºC did not alter progression of embryos to higher preimplantation stages (expanded and hatching/hatched blastocysts), rate of apoptosis, total cell number of blastocysts and structure of actin filament compared to 37.5 ºC. We stern-blotting revealed the presence of heat stress-induced 72 kDa fraction of Hsp70 proteins in granulosa cells (exposed to 41 ºC) and embryos (exposed to 41.5 ºC). Following the elevation of temperature to 42.5 ºC embryo development was dramati cally compromised. The embryos were arrested at the morula or early blastocyst stage, showed an increased rate of apoptosis and decreased total cell number compared to control. The structure of actin filaments in most of blastomeres was damaged and such blastomeres often contained apoptotic nuclei. In this group a presence of heat-stress-induced fraction of Hsp70 proteins had not been confirmed. This is the first report demonstrating a threshold of thermotolerance of rabbit preimplantation embryos to hyperthermic exposure in vitro. A detrimental effect of higher temperature on the embryo is probably associated with the loss of their ability to produce Hsp70 de novo, which leads to cytoskeleton alterations and enhanced apoptosis., A. V. Makarevich, L. Olexiková, P. Chrenek, E. Kubovičová, K. Fréharová, J. Pivko., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public