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Start Over Creator Jana Kolářová

1. Application of the optical method in experimental cardiology: action potential and intracellular calcium concentration measurement

Creator:
Ronzhina, M., Čmiel, V., Janoušek, O., Jana Kolářová, Marcela Nováková, Babula, P., and Ivo Provazník
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, optical method, fluorescent dyes, action potential, calcium concentration, 14, and 612
Language:
English
Description:
It has been shown that, in addition to conventional contact electrode techniques, optical methods using fluorescent dyes can be successfully used for cardiac signal measurement. In this review, the physical and technical fundamentals of the method are described, as well as the properties of the most common systems for measuring action potentials and intracellular calcium concentration. Special attention is paid to summarizing limitations and trends in developing this method., M. Ronzhina, ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Creator:
Jana Kolářová, Kateřina Fialová, Oto Janoušek, Marie Nováková, and Ivo Provazník
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, isolated heart, action potential, electrogram, voltage sensitive dye, ratiometry, 14, and 612
Language:
English
Description:
Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dualwavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented., J. Kolářová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Motion artefact in voltage-sensitive fluorescent dye emission during repeated ischemia of isolated heart

Creator:
Janoušek, O., Jana Kolářová, Ronzhina, M., Marie Nováková, and Ivo Provazník
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, motion artefact, ischemia, isolated rabbit heart, voltage-sensitive dye, 14, and 612
Language:
English
Description:
Motion artefact (MA) in voltage-sensitive fluorescent signals causes significant debasement of action potential. During ischemia and reperfusion in isolated rabbit heart, this artefact develops in a manner which may be described by the time of its onset, level, and shape. The MA during ischemia: (a) may become substantial with approximately two minutes delay after establishing global ischemia; (b) may be almost twice as high as the physiological action potential and decreases both with time and repetition of ischemia; (c) the MA shape is unpredictable and depends on individual rabbit., O. Janoušek, ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public