Search

Start Over Creator Křížová, E.

1. Effect of intermittent feeding with high-fat diet on changes of glycogen, protein and fat content in liver and skeletal muscle in the laboratory mouse

Creator:
Křížová, E. and Šimek, V.
Type:
article, model:article, and TEXT
Subject:
intermittent feeding, high-fat diet, metabolism, liver, and skeletal muscle
Language:
English
Description:
After 8 weeks of intermittent fasting, mice fed both a standard laboratory diet and a high-fat diet became hyperphagic and showed an increased amount of glycogen storage in the liver. An important effect of the adaptation to intermittent feeding with a high-fat diet seems to be an activation of the oxidation of lipids. Lipid oxidation prevails over lipogenesis so that the protein levels in the liver and skeletal muscle are preserved and maintained constant.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Influence of intermittent fasting and high-fat diet on morphological changes of the digestive system and on changes of lipid metabolism in the laboratory mouse

Creator:
Křížová, E. and Šimek, V.
Type:
article, model:article, and TEXT
Subject:
intermittent fasting, high-fat diet, total body fat, lipogenesis, and digestive system
Language:
English
Description:
The simultaneous effect of intermittent starvation and a high-fat diet were investigated in mice after several weeks of experimental feeding. The animals adapted to intermittent fasting fed a high-fat diet showed a lower degree of hyperphagia than animals adapted to intermittent fasting fed a standard laboratory diet. The weight of both individual portions of the stomach was elevated in adapted animals fed both a standard laboratory diet and the high-fat diet. The weight of the small intestine was increased in adapted animals fed a high-fat diet. The length of the small intestine was not changed after 8 weeks of intermittent starvation in both adapted groups (standard laboratory diet, high-fat diet). A higher amount of body fat was found in both groups of animals adapted to intermittent fasting (standard laboratory diet, high-fat diet) but adapted animals fed a high-fat diet showed less body fat than adapted animals fed a standard laboratory diet. Lower levels of serum lipids were found in adapted animals fed a high-fat diet. These results suggest that both lipogenesis and lipid oxidation are accentuated by intermittent starvation and a high-fat diet act concomitantly.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Visfatin is actively secreted in vitro from U-937 macrophages, but only passively released from 3T3-L1 adipocytes and HepG2 hepatocytes

Creator:
Svoboda, P., Křížová, E., Čeňková, K., Vápenková, K., Jarmila Zídková, Václav Zídek, and Vojtěch Škop
Format:
print, bez média, and svazek
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, nampt, visfatin, active secretion, adipocytes, hepatocytes, macrophages, 14, and 612
Language:
English
Description:
Visfatin is a multi-functional molecule that can act intracellularly and extracellularly as an adipokine, cytokine and enzyme. One of the main questions concerning visfatin is the mechanism of its secretion; whether, how and from which cells visfatin is released. The objective of this in vitro study was to observe the active secretion of visfatin from 3T3-L1 preadipocytes and adipocytes, HepG2 hepatocytes, U-937, THP-1 and HL-60 monocytes and macrophages. The amount of visfatin in media and cell lysate was always related to the intracellular enzyme, glyceraldehyde-3- phosphate dehydrogenase (GAPDH), to exclude the passive release of visfatin. Visfatin was not found in media of 3T3-L1 preadipocytes. In media of 3T3-L1 adipocytes and HepG2 hepatocytes, the ratio of visfatin to the amount of GAPDH was identical to cell lysates. Hence, it is likely that these cells do not actively secrete visfatin in a significant manner. However, we found that significant producers of visfatin are differentiated macrophages and that the amount of secreted visfatin depends on used cell line and it is affected by the mode of differentiation. Results show that 3T3-L1 adipocytes and HepG2 hepatocytes released visfatin only passively during the cell death. U-937 macrophages secrete visfatin in the greatest level from all of the tested cell lines., P. Svoboda, E. Křížová, K. Čeňková, K. Vápenková, J. Zídková, V. Zídek, V. Škop., and Obsahuje bibliografii
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public