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2. Genetic diversity and taxonomy of Sabanejewia balcanica (Osteichthyes: Cobitidae) in the waters of the Czech Republic and Slovakia
- Creator:
- Bartoňová, Eva, Papoušek, Ivo, Lusková, Věra, Koščo, Ján, Lusk, Stanislav, Halačka, Karel, Švátora, Miroslav, and Vetešník, Lukáš
- Type:
- article and TEXT
- Subject:
- Balcan spined loach, conservation, cytochrome b, and mitochondrial marker
- Language:
- English
- Description:
- Seven populations (Oľšava/Hornád R., Oľšava/Topľa R., Ublianka R., Ondava R., Ipeľ R., Malý Dunaj R. and Vlára R.) of Balcan spined loach were subjected to phylogenetic analysis based on variability of the cytochrome b gene and mitochondrial specific marker. They were separated in to five groups but these groups were represented by specimens from different populations. Genetic distances among populations and among groups were quite low. Subsequently, analysis of relations among our groups and sublineages of Danubian-Balkanian complex of Sabanejewia was made. the first four groups were genetically close to sublineage III, while the fifth one to the sublineage IV. These findings agree with the conclusion about conspecificity of analysed Danubian-Dniester populations belonged to monotypic S. balcanica. Phylogenetic analysis showed that the most suitable populations for the restoration of this species in the Bečva River are populations dwelling the Ipeľ and Vlára rivers.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
3. Genetic diversity of Cobitis spp (Cypriniformes: Cobitidae) from different drainage areas
- Creator:
- Papoušek, Ivo, Lusková, Věra, Koščo, Ján, Lusk, Stanislav, Halačka, Karel, Povž, Meta, and Šumer, Suzana
- Type:
- article and TEXT
- Subject:
- Danubian spined loach and population diversity
- Language:
- English
- Description:
- Genetic diversity of three pure populations of Cobitis elongatoides from hydrologically and geographically different drainage areas, the Lužnice River (48.49.60 N, 14.55.60 E, Labe River drainage area, North Sea basin), the Nová Říše reservoir (49.09.11 N, 15.32.464 E, Morava River drainage area, Black Sea basin), and the Ida River (48.32.284 N, 21.03.421 E, Tisza River drainage area, Black Sea basin) was evaluated by sequencing of mitochondrial cytochrome b gene. Species identification of unknown Cobitis samples from Vipava River drainage area in Slovenia was also performed based on cytochrome b sequence. All forms of the genus Cobitis are protected by national legislatives and, in accordance with the Council Directive No. 925/43/EEC, they enjoy protection in selected localities of European importance. In total, 11 haplotypes of Cobitis elongatoides were found in Czech and Slovak waters, manifesting low overall genetic diversity. Samples from Vipava River drainage area were identified as Cobitis bilineata.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
4. Genetic diversity of Misgurnus fossilis populations from the Czech Republic and Slovakia
- Creator:
- Mendel, Jan, Lusk, Stanislav, Koščo, Ján, Vetešník, Lukáš, Halačka, Karel, and Papoušek, Ivo
- Type:
- article and TEXT
- Subject:
- weather loach, endangered species, control region, cross-species amplification, and microsatellites
- Language:
- English
- Description:
- Monitoring of the analysed populations of the endangered fish species Misgurnus fossilis was conducted using microsatellite analysis and sequencing of a part of the control region. Absolutely first microsatellite markers for weather loach were found. Six polymorphic microsatellite loci were prepared, five of which were tested. Number of alleles per a locus ranged from 3 to 5. All studied populations are differentiated one from another (FST= 0.205 - 0.367). All sampled populations contained unique alleles. Sequential analysis of the mitochondrial control region showed great haplotype similarity of the studied populations which come from one widely spread haplotype H_1, and thus suggested possible hypothesis of recent spreading from one source.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/