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Start Over Subject acetylcholine release

1. Non-quantal acetylcholine release after cholinesterase inhibition in vivo

Creator:
Nikolsky, E. E., Oranska, T. I., and Vyskočil, F.
Type:
article, model:article, and TEXT
Subject:
acetylcholine release, mouse diaphragm, and anticholinesterases
Language:
English
Description:
After anticholinesterase treatment in vivo, depolarization of the postsynaptic muscle fibre membrane by about 4 mV develops due to non-quantally released acetylcholine from the motor nerve terminal. This conclusion was supported by experiments with the curarization of diaphragm slices from anticholinesterase treated mice during intracellular microelectrode recordings.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Voltage-dependent P/Q-type calcium channels at the frog neuromuscular junction

Creator:
Nurullin, L. F., Mukhitov, A. R., Tsentsevytsky, A. N., Petrova, N. V., Samigullin, D. V., Malomouzh, A. I., Bukharaeva, E. A., František Vyskočil, and Nikolsky, E. E.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, nervosvalový přenos, acetylcholin, neuromuscular transmission, acetylcholine, motor nerve terminal, acetylcholine release, N- and P/Q-type calcium channels, 14, and 612
Language:
English
Description:
It is well known that antagonists of N-type voltage-gated calcium channels inhibit the evoked quan tal release of acetylcholine in amphibian neuromuscular synapses. This, however, does not exclude the functional expression of other types of voltage-gated calcium channels in these nerve terminals. Using immunocytochemistry, we detected the expression of the α1A subunit of P/Q-type calcium channels (that is otherwise typical of mammalian motor nerve endings) in the frog neuromuscular junction. In addition, we demonstrated that the P/Q-type channel blocker ω-agatoxin IVA (20 nM) reduced the action potential- induced calcium transient and significantly decreased both spontaneous and evoked mediator release. Our data indicates the functional expression of P/Q-type calcium channels in the frog motor nerve ending which participate in acetylcholine release., L. F. Nurullin ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public