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Start Over Subject granulopoiesis

1. Combination of drugh elevating extracellular adenosine with granulocyte colony-stimulating factor promotes granulopoietic recovery in the murine bone marrow after 5-fluorouracil treatment

Creator:
Michal Hofer, Pospíšil, M., Lenka Weiterová, Vladimír Znojil, Vácha, J., Jiřina Holá, Antonín Vacek, and Iva Pipalová
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, granulocyty, granulocytes, 5-fluorouracil, granulopoiesis, extracellular adenosine, adenosine monophosphate, dipyridamole, granulocyte colony-stimulating factor, 14, and 612
Language:
English
Description:
Combined administration of drugs elevating extracellular adenosine, namely dipyridamole and adenosine monophosphate, together with granulocyte colony-stimulating factor was shown to enhance granulopoietic recovery in the bone marrow of mice treated with 5-fluorouracil. Enhanced regeneration was found both at the level of hematopoietic progenitor cells for granulocytes and macrophages and in the compartment of morphologically recognizable granulocyte precursors. The results might have positive clinical impact. The adjunct use of drugs elevating extracellular adenosine might reduce the cost expenditure of therapy with granulocyte colony-stimulating factor., M. Hofer, M. Pospíšil, L. Weiterová, V. Znojil, J. Vácha, J. Holá, A. Vacek, I. Pipalová., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice

Creator:
Lenka Weiterová, Michal Hofer, Milan Pospíšil, Vladimír Znojil, and Denisa Štreitová
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Biochemie. Molekulární biologie. Biofyzika, biofyzika, fyziologie živočichů, adenosin, biophysics, animal physiology, adenosine, granulopoeza, faktor stimulující kolonie granulocytů (G-CSF), interleukin-6 (IL-6), granulopoiesis, granulocyte colony-stimulating factor (G-CSF), interleukin 6 (IL-6), GM-CFC, serum colony-stimulating activity, 2, and 577
Language:
English
Description:
Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis., L. Weiterová, M. Hofer, M. Pospíšil, V. Znojil, D. Štreitová., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public