The impoundment located near the village of Poša in eastern Slovakia is a significant source of arsenic. Waters penetrating the impoundment become enriched in As and other potentially toxic elements. As a consequence, the Kyjov brook and the Ondava River have been extensively polluted by arsenic. Although, zinc is of minor environmental significance regarding pollution in the area, it was also monitored to compare its behaviour with that of arsenic. The mobility and solid-state distribution of As and Zn in the impoundment materials and stream sediments have been investigated using a five-step sequential extraction procedure. Moreover, to investigate the bioavailability of As and Zn, two native plant species (Typha latifolia and Phragmites australis) growing at the site were collected and analyzed. The As concentrations in representative sediment and water samples ranged from 36.28 to 3208.35 mg kg-1 and from 4.05 to 612.8 μg l-1, respectively, both being many times above the background levels. The Zn concentrations of environmental importance were found to be high only in the impoundment materials (up to 3390 mg kg-1). Although a part of As was present in a readily soluble form (6.62%), the majority of As was mainly associated with Fe/Mn oxides (37.30%) and residual phases (51%). Similarly, the most dominant fractions for zinc distribution were Fe/Mn oxides (38.5%), residual (34%) and exchangeable (18%). Combined results of the sequential extraction tests as well as chemical and mineralogical analysis indicated that As mobilisation potential from the sediments is likely controlled by Fe/Mn oxyhydroxide mineral phases. Plants growing in the impoundment had As concentrations 10 to 100 times higher than the same plants growing in a relatively nonpolluted area, indicating an enhanced bioavailability of arsenic in the area with high total As contents in the impoundment materials. and Odkalisko, ktoré sa nachádza pri obci Poša (východné Slovensko), je významným zdrojom arzénu. Vody presakujúce cez materiál uložený v odkalisku sa tak obohacujú o As a ďalšie potenciálne toxické prvky. Dôsledkom je výrazné znečistenie toku Kyjov a rieky Ondava arzénom. Aj keď v tejto oblasti zinok nepredstavuje až tak veľký environmentálny problém, je zahrnutý v tejto štúdii s cieľom porovnať správanie sa týchto dvoch potenciálne toxických prvkov. Na štúdium pohyblivosti a distribúcie As a Zn v materiáloch odkaliska a v riečnych sedimentoch sa použila päťkroková sekvenčná extrakcia. Okrem toho sme skúmali bioprístupnosť As a Zn v dvoch typoch rastlín (Typha latifolia a Phragmites australis), ktoré prednostne rastú na odkalisku. Koncentrácie As v odobratých vzorkách sedimentov boli v intervale od 36,28 do 3208,35 mg kg-1 a v povrchových vodách od 4,05 do 612,8 μg l-1. Tieto koncentrácie sú oveľa vyššie ako pozaďové hodnoty pre danú oblasť. Bolo zistené, že koncentrácie Zn významné z hľadiska znečistenia sú vysoké len v odkaliskových materiáloch (až 3390 mg kg-1). Aj keď určitý podiel As v sedimentoch bol prítomný v ľahko rozpustnej forme (6,62 %), väčšina As bola viazaná na oxidy Fe a Mn (37,30 %) a reziduálne fázy (51 %). Podobné to bolo pri Zn, pričom najdôležitejšie pre jeho distribúciu boli oxidy Fe a Mn (38,5 %), reziduálne fázy (34 %) a vymeniteľné pozície (18 %). Kombinované výsledky sekvenčných extrakcií ako aj chemickej a mineralogickej analýzy ukázali, že mobilizácia As zo sedimentov úzko súvisí s prítomnými minerálmi zo skupiny oxyhydroxidov Fe a Mn. Rastliny vyskytujúce sa na odkalisku obsahovali 10- až 100-násobne vyššie koncentrácie As ako tie isté rastliny odobraté z relatívne neznečistenej oblasti. Táto skutočnosť poukazuje na zvýšenú bioprístupnosť arzénu na skúmanom odkalisku, ktoré je typické vysokými obsahmi celkového As v uložených materiáloch.
Although the beneficial role of Fe, Zn, and Mn on many physiological and biochemical processes is well established, effects of each of these elements on chlorophyll (Chl) a fluorescence and photosynthetic pigment contents is not well studied. The objective of this study was to evaluate effects of Fe, Zn, and Mn deficiency in two lettuce cultivars. The parameters investigated could serve also as physiological and biochemical markers in order to identify stress-tolerant cultivars. Our results indicated that microelement shortage significantly decreased contents of photosynthetic pigments in both lettuce cultivars. Chl a fluorescence parameters including maximal quantum yield of PSII photochemistry and performance index decreased under micronutrient deficiency, while relative variable fluorescence at J-step and minimal fluorescence yield of the dark-adapted state increased under such conditions in both cultivars. Micronutrient deficiency also reduced all parameters of quantum yield and specific energy fluxes excluding quantum yield of energy dissipation, quantum yield of reduction of end electron acceptors at the PSI, and total performance index for the photochemical activity. Osmoregulators, such as proline, soluble sugar, and total phenols were enhanced in plants grown under micronutrient deficiency. Fe, Zn, and Mn deficiency led to a lesser production of dry mass. The Fe deficiency was more destructive than that of Zn and Mn on the efficiency of PSII in both lettuce cultivars. Our results suggest that the leaf lettuce, which showed a higher efficiency of PSII, electron transport, quantum yield, specific energy fluxes, and osmoregulators under micronutrient deficiency, was more tolerant to stress conditions than crisphead lettuce., H. R. Roosta, A. Estaji, F. Niknam., and Obsahuje bibliografii
Natural and commercial Salix clones differ in their ecophysiological response to Zn stress This study was carried out to determine the effect of different zinc concentrations on the ecophysiological response of Salix clones: four commercial clones (“1962”, “1968”, “Drago”, and “Levante”) selected for short rotation coppice, and one natural clone, “Sacco”, obtained from a contaminated area. Gas exchanges, chlorophyll a fluorescence (JIP-test), relative chlorophyll content, and biometric parameters were measured in plants grown for fifteen days in soil containing Zn concentrations of 0, 300, 750, and 1,500 mg(ZnCl2) kg-1. Ecophysiological response to metal stress differed in dependence on the Zn concentration and clone. At the low Zn concentration (300 mg kg-1), the absence of any significant reductions in parameters investigated indicated an efficient plant homeostasis to maintain the metal content within phytotoxic limits. Stomatal limitation, observed at 750 and 1,500 mg kg-1, which was found in all clones after three days of the treatment, might be caused by indirect effects of metal on guard cells. Among commercial clones, “Drago” was more sensitive to Zn stress, showing inhibition of growth, while “1962” clone showed a downregulation of PSII photochemistry following the slowdown in the Calvin-Benson cycle. On the contrary, the natural Salix clone (“Sacco”) performed better compared to the other clones due to activation of a photosynthetic compensatory mechanism., A. Bernardini, E. Salvatori, S. Di Re, L. Fusaro, G. Nervo, F. Manes., and Obsahuje seznam literatury
The toxicity of cadmium and zinc at concentrations ranging from 0.1 to 100 µg/l was investigated against the activity of Diplostomum spathaceum (Rudolphi, 1819) cercariae. Over a 24 h exposure period a significant reduction in cercarial activity occurred in solutions of cadmium, zinc, and a mixture of cadmium and zinc at all concentrations. Reduced cercarial activity also occurred in all toxicant solutions compared with controls after only 6 h exposure indicating that cercariae were vulnerable during the period of maximum cercarial infectivity (0-5 h). The mechanisms of metal toxicity and their importance to parasite transmission are discussed.
The protective role of nutrition factors such as calcium, vitamin D and vitamin K for the integrity of the skeleton is well understood. In addition, integrity of the skeleton is positively influenced by certain trace elements (e.g. zinc, copper, manganese, magnesium, iron, selenium, boron and fluoride) and negatively by others (lead, cadmium, cobalt). Deficiency or excess of these elements influence bone mass and bone quality in adulthood as well as in childhood and adolescence. However, some protective elements may become toxic under certain condition s, depending on dosage (serum concentration), duration of treatment and interactions among individual elements. We review the beneficial and toxic effects of key elements on bone homeostasis., I. Zofkova, M. Davis, J. Blahos., and Obsahuje bibliografii
Cadmium in the dose of 1 mg/animal/day was administered to guinea-pigs in the form of CdCl2 dissolved in drinking water during short-term (5 weeks) and subchronic (12 weeks) experiments. Both the control and cadmium-treated groups were divided into two subgroups, according to low (2 mg/animal/day) and high (100 mg/animal/day) vitamin C intake. Subchronic cadmium treatment caused copper deficiency indicated by a dramatic decrease of copper concentration in the liver and serum and by its moderate decrease in the testes and brain. Cadmium significantly increased zinc concentrations in the kidneys during the whole experiment and decreased the level of zinc in the serum after subchronic cadmium treatment. In the control groups, the levels of zinc and copper in the serum were lowered after 5 weeks of high vitamin C doses. High doses of vitamin C in cadmium-treated guinea-pigs decreased the levels of copper in the testes, brain and serum. These observations suggest that cadmium intake in relatively high doses might potentiate the development of copper deficiency, and high doses of vitamin C aggravate, to a certain extent, copper depletion in some important organs and serum of guinea-pigs.